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关键词： HLA-C*17:69   novel allele   sequencing-based typing  

摘要： HLA-C*17:69 differs from HLA-C*17:01:01:02 by one nucleotide in exon 4. © 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

关键词： Autism spectrum disorder   IL-17A   MMP9   Quercetin   TRAF6   Yigansan  

摘要： Background: Maternal immune activation (MIA) is a significant factor inducing to autism spectrum disorder (ASD) in offspring. The fundamental principle underlying MIA is that inflammation during pregnancy impedes fetal brain development and triggers behavioural alterations in offspring. The intricate pathogenesis of ASD renders drug treatment effects unsatisfactory. Traditional Chinese medicine has strong potential due to its multiple therapeutic targets. Yigansan, composed of seven herbs, is one of the few that has been proven to be effective in treating neuro-psychiatric disorders among numerous traditional Chinese medicine compounds, but its therapeutic effect on ASD remains unknown. Hypothesis: Yigansan improves MIA-induced ASD-like behaviours in offspring by regulating the IL-17 signalling pathway. Methods: Pregnant C57BL/6J mice were intraperitoneally injected with poly(I:C) to construct MIA models and offspring ASD models. Network analysis identified that the IL-17A/TRAF6/MMP9 pathway is a crucial pathway, and molecular docking confirmed the binding affinity between the monomer of Yigansan and target proteins. qRT-PCR and Western blot were used to detect the expression levels of inflammatory factors and pathway proteins, immunofluorescence was used to detect the distribution of IL-17A, and behavioural tests were used to evaluate the ASD-like behaviours of offspring. Results: We demonstrated that Yigansan can effectively alleviate MIA-induced neuroinflammation of adult offspring by regulating the IL-17A/TRAF6/MMP9 pathway, and the expression of IL-17A was reduced in the prefrontal cortex. Importantly, ASD-like behaviours have been significantly improved. Moreover, we identified that quercetin is the effective monomer for Yigansan to exert therapeutic effects. Conclusion: Overall, this study was firstly to corroborate the positive therapeutic effect of Yigansan in the treatment of ASD. We elucidated the relevant molecular mechanism and regulatory pathway involved, determined the optimal therapeutic dose and

关键词： Antimicrobial   Autoimmunity   IL-26   Skin inflammation   Th17  

摘要： T helper 17 (Th17) cells produce a set of cytokines that include IL-17 family members, IL-21, IL-22, and IL-26. These cytokines all contribute to the classic function of Th17 cells in combatting extracellular infection and promoting inflammation in autoimmune diseases. However, of the Th17 cytokines, only IL-26 has direct antimicrobial activity against microbes and can activate a broad range of immune cells through its ability to bind DNA and trigger pattern recognition receptors. It is noteworthy that IL-26 is produced by mammalian cells, including human Th17 cells, but is absent in rodents. As such, IL-26 is a potential therapeutic target to augment host immune responses against microbial pathogens but also to prevent inflammation and tissue damage in a variety of autoimmune diseases. © 2023 The Authors

关键词： biomarker   immuno-profile   post-acute sequela of COVID-19   SARS-CoV-2  

摘要： Background: Post-acute sequelae of COVID-19 (PASC) comprise a broad spectrum of symptoms such as fatigue, general weakness, compromised attention and sleep or anxiety disorders. PASC represents a medical and socio-economic challenge.Objectives: Our study evaluated cytokines (IL-1 beta, IL-6 and TNF alpha) and cortisol levels in a cohort of typical patients with PASC, suffering concentration problems, fatigue and difficulties finding words.Design: This was a prospective cohort study. Four groups were analysed and compared: those who had never contracted SARS-CoV-2 (n = 13), infected but had no PASC (n = 34), infected with former PASC that resolved (n = 40) and patients with ongoing PASC after infection (n = 91).Methods: Cytokine and cortisol serum levels were determined in patients' blood samples.Results: Cytokine levels of IL-1 beta, IL-6, TNF alpha and cortisol levels did not differ between groups analysed.Conclusion: This may indicate a non-organic/psychosomatic genesis of PASC; further studies are needed to elucidate the underlying causes of PACS, and non-organic causes should not be overlooked. Without clear biological markers for people who will continue to present with post-acute sequelae of COVID-19 (PASC) should we now focus on psychological factors?Many people across the globe are still suffering from post-acute sequelae of COVID-19 (PASC), commonly called post-COVID. Typical symptoms of PASC include severe tiredness (fatigue), concentration deficits (brain fog) or difficulty finding words. We need a better understanding of how these symptoms arise to find ways to help patients. Our team of researchers set out to explore this. We posed the question: could measurements of immune system activity provide an identifier for people who are susceptible to post-COVID?The participants in our study were divided into four groups: 1. A group of 13 people who had never contracted SARS-CoV-2. 2. A group of 34 people who had been infected with SARS-CoV-2 but had no PASC. 3. A group of 40 people who had been infected wi

关键词： Biomarker   Clinical parameters   Cytokines   Peri-implant disease  

摘要： Peri-implant disease (PID) is a general term for inflammatory diseases of soft and hard tissues that occur around implants, including peri-implant mucositis and peri-implantitis. Cytokines are a class of small molecule proteins, which have various functions such as regulating innate immunity, adaptive immunity, and repairing damaged tissues. In order to explore the characteristics and clinical significance of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and tumor growth factor (TGF)-β1 expression levels in serum of patients with peri-implant disease, 31 patients with PID and 31 patients without PID were enrolled. The modified plaque index (mPLI), modified sulcus bleeding index (mSBI), and peri-implant probing depth (PD) were recorded. The levels of serum TNF-α, IL-6, IL-10, and TGF-β1 were detected by ELISA. TNF-α, mPLI, mSBI, and PD levels were significantly higher in the PID group. TGF-β1 levels were significantly higher in the control group. There was a significant positive correlation between TNF-α and mPLI, mSBI, and PD. TGF-β1 was negatively associated with TNF-α, mPLI, mSBI, and PD. Multiple logistic regression analysis showed that TNF-α and PD were risk factors for the severity of PID. The receiver operating curve analysis showed that high TNF-α levels (cut-off value of 140 pg/mL) and greater PD values (cut-off value of 4 mm) were good predictors of PID severity with an area under the curve of 0.922. These results indicated that TNF-α and PD can be used as a biological indicator for diagnosing the occurrence and progression of PID. © 2024, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.

关键词： Antiviral response   Interferon   Virus   Zebrafish  

摘要： Interferons (IFNs) are a group of secreted cytokines that play a crucial role in antiviral immunity. Type I IFNs display functional disparities. In teleosts, type I IFNs are categorized into two subgroups containing one or two pairs of disulfide bonds. However, their functional differences have not been fully elucidated. In this study, we comparatively characterized the antiviral activities of zebrafish IFNφ1 and IFNφ4 belonging to the group I type I IFNs. It was found that ifnφ1 and ifnφ4 were differentially modulated during viral infection. Although both IFNφ1 and IFNφ4 activated JAK-STAT signaling pathway via CRFB1/CRFB5 receptor complex, IFNφ4 was less potent in inducing phosphorylation of STAT1a, STAT1b and STAT2 and the expression of antiviral genes than IFNφ1, thereby conferring weaker antiviral resistance of target cells. Taken together, our results provide insights into the functional divergence of type I IFNs in lower vertebrates. © 2024 Elsevier Ltd

关键词： NLRP3-inflammasome   Western blotting   antibody   microvesicles (MVs)   tissue factor (TF)  

关键词： Abdominal scan   Bowel neoplasm   Intussusception   Mechanical obstruction  

摘要： Intussusception is rare in adults, accounting for 1 to 5 % of mechanical bowel obstructions. It is due to pathologic lead point within the bowel which is malignant in up to 77 % of cases. Benign lesions may also be responsible for intussusception (polyp, Meckel diverticulum). The lead point is pulled forward by normal peristaltism, prolapsing the affected segment of bowel into another segment. The most common presentation in adults is intermittent abdominal pain and bowel obstruction (nausea, vomiting, inability to pass gas or stools). Abdominal scanner is the key exam for the diagnosis and the treatment is always surgical resection.; Les invaginations grêles et coliques sont rares chez l’adulte, représentant 1 à 5 % des causes d’obstruction digestive mécanique. Elles sont le plus souvent causées par une masse intra-digestive, le péristaltisme entraînant un prolapsus du segment malade vers un segment intestinal adjacent. Elles sont généralement le reflet d’une pathologie maligne du tube digestif (tumeur maligne découverte dans plus de 77 % des cas), mais il peut également exister des causes béniqnes (polype, diverticule de Meckel, adénopathie) . Elles se manifestent sous forme de douleurs abdominales et d’occlusion (nausées, vomissements, arrêt des selles et des gaz). Le scanner abdominal est l’examen de choix pour poser le diagnostic. Le traitement est toujours une résection chirurgicale.

摘要： 贝林妥欧单抗是一种同时靶向CD3和CD19的双特异性抗体, 能激活内源性T细胞杀伤B系肿瘤细胞。自2014年美国食品药品监督管理局批准用于治疗复发难治性急性淋巴细胞白血病(ALL)以来, 临床应用广泛, 应用的指征不断前移, 疗效和安全性均较好。在儿童白血病领域, 除复发难治性ALL外, 目前已经用于微小残留病阳性ALL的治疗以及高危ALL的一线治疗, 同时也用于化疗不耐受患儿的治疗, 并可桥接造血干细胞移植。本文就贝林妥欧单抗在儿童ALL治疗方面的临床研究进展进行综述。

关键词： T细胞   免疫相关性不良反应   非小细胞肺癌   免疫检查点抑制剂  

摘要： 目的 分析使用免疫检查点抑制剂(ICI)治疗后出现免疫相关不良反应(irAE)的非小细胞肺癌(NSCLC)患者的外周血T淋巴细胞亚群水平及其与血清细胞因子的关系。方法 前瞻性纳入2022年4～10月于某院就诊的非小细胞肺癌患者47例，其中根据有无发生irAE分为irAE组(n=12)和对照组(n=35)，采用流式细胞术检测患者外周血淋巴细胞亚群的比例，比较两组间淋巴细胞亚群比例的差异性，Pearson相关性分析细胞因子与淋巴细胞亚群水平的相关性。结果 与对照组相比，irAE组患者外周血TCR-αβ+PD1+CD4-CD8-T细胞水平升高，差异具有统计学意义(P=0.045)，其他T淋巴细胞亚群无明显差异。Pearson相关性分析结果显示外周血TCR-αβ+PD1+CD4-CD8-T细胞与血清干扰素-γ(IFN-γ)呈正相关关系(r=0.448;P=0.019)。结论irAE患者外周血TCR-αβ+PD1+CD4-CD8-T细胞水平升高，并且与血清IFN-γ水平正相关，有可能参与irAE的发生。