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摘要： INTRODUCTION & OBJECTIVES: Renal cell carcinoma (RCC) represents the majority of kidney malignancies and is characterized by variable outcomes, even with current systemic therapies. Interleukin-6 (IL-6), a proinflammatory cytokine implicated in tumor progression and immune suppression, has been proposed as a prognostic biomarker in RCC. However, the evidence remains inconsistent due to methodological heterogeneity across studies. Therefore, our study aims to evaluate the prognostic significance of IL-6 in RCC by synthesizing data from published studies, specifically overall survival (OS) and progression-free survival (PFS). METHODS: A systematic meta-analysis was conducted to evaluate the prognostic significance of IL-6 in RCC. Eligible studies were identified through PubMed, ScienceDirect, and ProQuest up to March 2025. Inclusion criteria encompassed original articles measuring pre-treatment serum IL-6 levels in RCC patients and reporting associations with overall survival (OS) or progression-free survival (PFS). Random-effects models were used to compute pooled hazard ratios (HRs) and survival differences. RESULTS: Nine studies comprising 702 RCC patients were included. Patients with low IL-6 levels had significantly longer OS (difference: 5.36 months;95% CI: 2.2-8.53;p<0.001;I² = 0%) and PFS (difference: 6.41 months;95% CI: 1.3-11.53;p=0.01;I² = 48.5%) compared to those with high IL-6. The pooled HR for survival associated with elevated IL-6 was 2.06 (95% CI: -0.23-4.36), with considerable heterogeneity (I² = 89.19%) and borderline statistical significance (p=0.08). Despite variations in study design, sample size, and IL-6 detection methods, elevated IL-6 consistently predicted worse clinical outcomes. CONCLUSIONS: IL-6 is a promising prognostic biomarker in RCC, with elevated levels associated with significantly poorer OS and PFS.

关键词： Molluscs  

摘要： Marine food is well known to be rich in bioactive molecules that can aid in the prevention and treatment of chronic inflammatory diseases. Among them, bivalve mollusks such as the Pacific oyster (Crassostrea gigas) have been receiving growing interest due to their high nutritional and medicinal value. This study evaluated the chemical composition of Pacific oyster soft tissue extract (OE) and investigated its potential anti-inflammatory effects on human intestinal epithelial cells. The nutritional value including moisture, total protein, ash, total lipids, fatty acids (FAs), amino acids and minerals was analyzed, as well as polyphenol and carotenoid contents. The potential anti-inflammatory activity was tested at 5, 50, and 500 mu g mL-1 concentrations of OE against TNF-alpha induced inflammation in the intestinal human epithelial cell line Caco-2. OE was shown to reduce both the TNF-alpha induced activation of the NF-kappa B pathway and the alteration of the epithelial barrier integrity. The present findings might provide evidence for further understanding how whole dried oyster meat can be developed as a low-cost nutraceutical dietary supplement and thus can offer a natural alternative to alleviate intestinal inflammation associated with different chronic diseases.

关键词： Plant extracts   Oxidative stress   IL-17 pathway   TGF-beta/Smad pathway   Photoaging  

摘要： BackgroundEucommia ulmoides Oliv. (PE), Styphnolobium japonicum (L.) Schott (PS) and Portulaca oleracea L. (PP) are well-characterized medicinal botanicals with established therapeutic profiles. PE exhibits anti-psoriatic and anti-aging properties through bioactive constituents, as demonstrated in vitro and in vivo research. PS flowers contain sophoricoside, a validated modulator of atopic dermatitis pathways, while PP displays broad-spectrum anti-inflammatory and antioxidant activities. Given their complementary mechanisms and the limited efficacy of single-agent approaches against UVB-driven oxidative stress/inflammation, we hypothesized that a tripartite extract (ESP: PE/PS/PP) would demonstrate synergistic photoprotection. This study evaluated the efficacy of ESP through integrated experimental cell models, a murine model of acute UVB-induced photoaging, and transcriptome *** extracts demonstrated synergistic antioxidant and photoprotective activities through multi-target mechanisms, effectively attenuating UVB-induced photoaging in vitro and in vivo. LC-QTOF-MS analysis identified major bioactive components comprising organic acids, polysaccharides, iridoids, and flavonoids. In a paracrine signaling model using conditioned medium from UVB-exposed HaCaT cells to stimulate HFF cells, ESP significantly inhibited UVB-induced secretion of inflammatory mediators (PGE2, TNF-alpha, IL-1 alpha, IL-6). Transcriptome sequencing coupled with RT-qPCR validation revealed that ESP attenuated photoaging via the IL-17 signaling pathway, downregulating key mediators including matrix metalloproteinases (MMP1/3/13), chemokines (CXCL3/8, CCL7), and colony-stimulating factors (CSF2/3). Furthermore, ESP reduced mitochondrial ROS generation, stabilized membrane potential, activated the TGF-beta/Smad pathway, and upregulated COL1A1 and COL3A1 expression. Significantly, in a murine model of acute UVB-induced photoaging, ESP treatment produced dose-dependent restoration o

关键词： Chlamydia pneumoniae   ER stress   IL-10   IRE1α   neutrophil  

摘要： Neutrophils are among the first cells to be recruited to the lungs during infection in mouse models; however, their regulatory functions are not yet fully understood. This study examined the mechanisms and significance of IL-10-producing neutrophils throughout pulmonary infection in C57BL/6 mice. Our findings revealed that infection with induces IL-10 secretion in bone marrow-derived neutrophils, depending on Toll-like receptor 2 (TLR2) activation. This process involves TLR2-dependent mitochondrial reactive oxygen species (ROS) production, which triggers the endoplasmic reticulum (ER) stress pathway, including IRE1α and subsequent Xbp1 splicing. Inhibition of this pathway or depletion of neutrophils (using the 1A8 monoclonal antibody) significantly reduces IL-10 levels in bronchoalveolar lavage fluid (BALF) in vivo. Conversely, the absence of IL-10-producing neutrophils, whether through depletion or TLR2 deficiency, leads to increased IL-12p70 and IFN-γ-positive NK cells, along with decreased regulatory T cells and M2-like macrophages. This results in a lower bacterial burden in the lungs but causes more severe pulmonary damage and decreased survival rates. These findings highlight that IL-10 produced by neutrophils via the TLR2-mitochondrial ROS-ER stress pathway is essential for modulating pulmonary immune responses and maintaining immune homeostasis during infection, thereby preventing excessive inflammation and tissue damage.

关键词： HLA-A*11:494   Indian   next-generation sequencing   novel allele  

摘要： The novel allele HLA-A*11:494 differs from HLA-A*11:01:01:01 by one non-synonymous substitution, c.G589A in Exon 3.

关键词： 胃癌   中医健脾养胃法   癌胚抗原   中医症候积分   临床疗效  

摘要： 研究中医健脾养胃法对胃癌患者癌胚抗原水平及中医症候积分等方面的调控效应。方法 本研究于2023年10月至2025年3月开展,将60例胃癌患者随机分组:参照组(30例)采用常规化疗,调研组(30例)在化疗基础上联合中医健脾养胃疗法,以比较两种方案的疗效差异。结果 疗效对比结果显示,调研组的综合临床疗效显著高于对照组(P<0.05)。在中医症候改善方面,两组治疗前评分相当(P>0.05),但治疗后调研组症候积分明显降低(P<0.05)。肿瘤标志物检测显示,两组在治疗前、后均未呈现统计学差异(P>0.05)。胃肠激素水平分析表明,虽然两组基线数据相近(P>0.05),但治疗后调研组胃肠激素水平提升更为显著(P<0.05)。结论 中医健脾养胃法联合化疗治疗胃癌效果显著,能更快改善临床症状,调节肿瘤标志物和胃肠激素水平,有效延缓疾病进展。

关键词： Azeri population   IL-10   IRF5   Palindromic rheumatism   PTPN22   Rheumatoid arthritis   Single-nucleotide polymorphisms   TNFAIP3  

摘要： ObjectivePalindromic rheumatism (PR) is characterized by frequent and irregular attacks of pain and swelling in the joints and peri-articular structures. In this study, we investigated TNFAIP3, PTPN22, IRF5, and IL-10 genes' single-nucleotide polymorphisms (SNPs) in patients with *** a cross-sectional study, patients with a diagnosis of PR and two control groups, including rheumatoid arthritis (RA) patients and healthy controls (HCs), were recruited. All participants were Azeri. The TNFAIP3 (rs2230926, rs5029937), PTPN22 (rs1217407, rs1217413), IRF5 (rs10954213, rs2004640), and IL-10 (rs1800872, rs1800896) gene SNPs were *** SNPs were genotyped in 25 PR patients, 41 RA patients, and 40 HCs. The TT genotype of TNFAIP3 rs2230926 and GG genotype of IL10 rs1800872 polymorphisms were less common in the PR group compared to the RA and HC groups. The C allele of TNFAIP3 rs2230926 polymorphism was more common in the PR group compared to the RA and HC groups. The TC genotype and C allele of IL10 rs1800896 and AG genotype of IRF5 rs10954213 polymorphisms were more common in the PR group compared to the HCs;however, there were no significant differences between the PR and RA groups. No significant difference was observed in the distribution of TNFAIP3 rs5025937, IRF5 rs2004640, PTPN22 rs1217413, and PTPN22 rs1217407 genotypes and alleles between the studied *** C allele of TNFAIP3 rs2230926, CC and TC genotypes and C allele of IL10 rs1800896, and AG genotype of IRF5 rs10954213 polymorphisms are associated with PR in the Azeri population. Key Points center dot The TT genotype of the TNFAIP3 rs2230926 polymorphism is less common in patients with palindromic rheumatism than in patients with rheumatoid arthritis and healthy *** dot The GG genotype of the IL10 rs1800872 polymorphism is less common in patients with palindromic rheumatism than in patients with rheumatoid arthritis and healthy *** dot The

关键词： 异型淋巴细胞   形态学特征   病毒感染   外周血涂片   诊断  

摘要： 探讨外周血涂片中异型淋巴细胞形态学特征与不同病毒感染类型的关系,为临床诊断提供参考依据。方法 选取2024年1月至2025年6月收治的94例病毒感染患者,根据病毒感染类型分为EB病毒感染组(47例)和流感病毒感染组(47例),对两组患者外周血涂片中异型淋巴细胞的形态学特征进行观察分析,包括细胞大小、核质比、胞质特征、核形态等指标。结果 EB病毒感染组异型淋巴细胞以大型细胞为主(78.72%),核质比高,胞质呈深蓝色,核形态不规则;流感病毒感染组异型淋巴细胞以中等大小为主(68.09%),核质比相对较低,胞质呈浅蓝色,核形态相对规则,两组比较差异具有统计学意义(P<0.05)。结论 不同病毒感染类型引起的异型淋巴细胞形态学特征存在明显差异,通过外周血涂片形态学分析有助于辅助临床诊断。

摘要： Celiac disease (CD) is a chronic condition triggered by gluten consumption in genetically predisposed individuals, with approximately 95% of CD patients carrying the HLA-DQ2 or DQ8 risk haplotypes. In addition to genetic susceptibility, recent evidence suggests that these haplotypes may influence the clinical presentation and severity of CD. However, evidence on this topic remains scarce, and the impact of HLA-DQ2 and DQ8 haplotypes on CD is not yet fully established. The present study investigated the relationship between HLA-DQ2 and DQ8 haplotypes and gastrointestinal symptoms in CD patients from Southern Brazil, treated in a private clinical setting. All patients were diagnosed with CD based on positive serological tests (anti-endomysial IgA) and confirmed by histological findings in duodenal biopsies (Marsh classification). The presence of haplotypes was determined by DNA typing using polymerase chain reaction (PCR), and clinical data were collected at the initial consultation, prior to the initiation of a gluten-free diet.

关键词： α-亚麻酸   Th17细胞   作用机制   免疫调节   营养代谢   细胞互作  

摘要： 作为具有免疫调节功能的多不饱和脂肪酸，α-亚麻酸（ALA）在慢性炎症性疾病中显示出多重调控效应。Th17细胞作为CD4+T淋巴细胞的重要亚群，其异常活化与多种自身免疫性疾病的病理进程密切相关。本文系统阐述Th17细胞的生物学特性及其在免疫稳态失衡中的作用机制，重点总结ALA通过多靶点途径调节Th17细胞功能的分子基础，如：ALA可通过调控核受体RORγt的转录活性，抑制Th17细胞的分化进程并减少其细胞数量，从而降低IL-17、IL-21等促炎介质的生成。ALA可以通过干扰STAT3信号通路的磷酸化激活，阻断炎症信号传导。同时，ALA还可通过调节Th17细胞的脂质代谢通路和能量代谢途径，改变细胞的代谢特征，进而影响其免疫活性。本文通过对ALA在Th17细胞功能的多方面调控机制进行综述，指导Th17细胞介导的ALA精准营养干预策略，并为畜牧动物的健康养殖提供新思路。