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关键词： 组织液   内环境   生成机制   组成功能   淋巴细胞  

摘要： 本文围绕生物体内环境中的组织液展开,详细阐述其来源与生成机制,包括组织液源自血浆、通过毛细血管壁滤过形成,以及在动态平衡中受多种压力调节的过程;分析组织液的组成成分(如水、电解质、营养物质等)及其在维持细胞代谢、信息传递等方面的功能;探讨淋巴细胞在组织液中的活动,包括其进入组织液的方式及在免疫监视、应答等方面的作用.同时结合典型例题加深对相关知识的理解,旨在明确组织液对维持内环境稳态及机体健康的重要意义.

关键词： complex medical dermatology   cryoglobulinemia   monoclonal gammopathy   necrobiotic xanthogranuloma   scleromyxedema   CTD   connective tissue disease   EED   erythema elevatum diutinum   IBD   inflammatory bowel disease   Ig   immunoglobulin   IL   interleukin   IVIG   intravenous immunoglobulin   LCV   leukocytoclastic vasculitis   MG   monoclonal gammopathy   MGCS   monoclonal gammopathy of cutaneous significance   MGUS   monoclonal gammopathy of undetermined significance   NXG   necrobiotic xanthogranuloma   PG   pyoderma gangrenosum   SPD   subcorneal pustular dermatosis   SS   Sweet syndrome   UPEP   urine protein electrophoresis  

摘要：

关键词： Inflammatory bowel disease   3D bioprinting   TNF-alpha siRNA   Silk fibroin   Hydrogels  

摘要： Inflammatory bowel disease (IBD) is a chronic, relapsing disease that poses significant challenges in treatment. This study aimed to develop silk fibroin-based mesalazine and chitosan:TNF-alpha siRNA polyplex-loaded, 3D bioprinted hydrogels for the oral treatment of IBD. For this purpose, bioink formulations composed of silk fibroin, hyaluronic acid, and sodium alginate were optimized. A chitosan:TNF-alpha siRNA polyplex was also formulated at a 40:1 chitosan-to-siRNA ratio. Hydrogel formulations were fabricated using 3D bioprinting and characterized in terms of compatibility, thermal stability, swelling behavior, degradation, mechanical properties, and mucoadhesion to both healthy and IBD-induced colon tissues. The optimized oral hydrogel (H-M-P12) demonstrated a swelling index of 366 +/- 67 % and underwent 31.2 % degradation after 24 h in vitro. Mesalazine and TNF-alpha siRNA exhibited a sustained release profile from the hydrogels. Cytotoxicity studies confirmed the biocompatibility of the hydrogels and a TNF-alpha gene silencing efficiency of 46.53 % was obtained. In vivo studies in a Balb/c mouse model of IBD revealed significant improvements in physiological parameters, macroscopic and microscopic morphology, and biochemical markers following treatment with the developed hydrogels. These findings suggest that silk fibroin-based hydrogels incorporating mesalazine and chitosan/TNF-alpha siRNA polyplex, produced via 3D bioprinting, hold promise as an effective therapeutic approach for IBD.

关键词： Dupilumab   IgG4-related disease   IgG4-related orbital disease   IgG4-RD   IgG4-related disease   IL   interleukin  

摘要： An immunohistochemical evaluation of the patient’s left submandibular gland was infiltrated dominantly with IgG + cells ( A) and IgG4 + cells ( B). IgG4 +/IgG + ratio was greater than 50%, and IgG4 + cells greater than 100/ high-power field. Origi...

关键词： 早期糖尿病肾脏病   25   羟维生素D_(3)   胱抑素C   IL-6  

摘要： 目的:探究25-羟维生素D_(3)[25-(OH)D_(3)]、胱抑素C(Cys C)、IL-6在早期糖尿病肾脏病中的临床诊断价值。方法:纳入上饶市立医院2021年8月—2023年9月就诊的104例糖尿病患者入糖尿病组,另纳入50例同期于本院行体检的志愿者入对照组,采集所有参检者静脉血后测定血清25-(OH)D_(3)、Cys C、IL-6水平。对比两组IL-6、25-(OH)D_(3)、Cys C水平;对比糖尿病肾脏病不同发展阶段患者IL-6、25-(OH)D_(3)、Cys C水平;分析IL-6、25-(OH)D_(3)、Cys C及联合检测在早期糖尿病肾脏病中的诊断价值。结果:相比于对照组,糖尿病组IL-6、Cys C水平较高,25-(OH)D_(3)水平较低,差异有统计学意义(P<0.05)。早期糖尿病肾脏病患者IL-6及Cys C水平高于正常蛋白尿患者,且低于临床糖尿病肾脏病患者,25-(OH)D_(3)水平低于正常蛋白尿患者,且高于临床糖尿病肾脏病患者,差异有统计学意义(P<0.05)。ROC曲线显示,三者联合检测在早期糖尿病肾脏病中的诊断价值较高,AUC为0.943,敏感度为0.875,特异度为0.820,之后依次为IL-6、Cys C、25-(OH)D_(3)。结论:早期糖尿病肾脏病诊断中应用血清25-(OH)D_(3)、Cys C、IL-6联合检测可获得较高的临床应用价值,依据各指标水平的变化有助于更好地判断糖尿病发展阶段,为临床制定针对性治疗方案等提供有效的参考。

关键词： 持续正压通气   阻塞性睡眠呼吸暂停低通气综合征   白细胞介素-23  

摘要： 目的 探讨持续气道正压通气治疗阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者的效果及对机体血清白细胞介素-23(IL-23)的影响。方法 回顾性分析60例OSAHS患者的资料,将常规内科治疗的30例患者设为对照组,常规治疗基础上持续正压通气治疗的30例患者设为观察组。组间对比效果。结果 观察组的治疗有效率高于对照组(P<0.05);治疗后,两组患者睡眠质量各指标均有改善,且观察组更明显(P<0.05);治疗后,两组患者血清水平均有改善,且观察组更明显(P<0.05)。结论 持续正压通气治疗在OSAHS患者治疗中具有显著优势,可提高治疗有效率,能有效改善睡眠质量,对调节机体血清白细胞介素-23(IL-23)有积极作用。

关键词： 骨关节炎,膝   T2 mapping   白细胞介素-1   磁共振成像  

摘要： 目的:探讨早期膝骨关节炎软骨MRI T2-mapping成像测量的T2值与滑液中白细胞介素1(IL-1)含量的相关性。方法:选取经临床诊断的早期膝骨关节炎患者46例,行膝关节常规序列及T2-mapping序列扫描,经图像后处理软件测量股骨承重面、非承重面软骨区及胫骨内外侧髁T2值,采用化学发光法测定滑液中IL-1的含量,分析T2值与IL-1含量的相关性。结果:膝骨关节炎股骨内髁承重面关节软骨T2值为40.51~54.43 ms,平均(47.87±2.77)ms;非承重面关节软骨T2值为43.01~47.71 ms,平均(45.31±2.17)ms。股骨外髁承重面关节软骨T2值为39.86~48.43 ms,平均(44.87±3.27)ms;非承重面关节软骨T2值为44.31~48.91ms,平均(46.31±3.37)ms。胫骨内髁关节软骨T2值为39.49~51.21ms,平均(46.31±2.67)ms;胫骨外髁关节软骨T2值为40.59~46.71 ms,平均(43.19±2.67)ms。膝关节滑液IL-1含量为36.66~72.82 pg/mL,平均(54.76±30.05)pg/mL。股骨内髁承重面及胫骨内髁关节软骨T2值与滑液中IL-1含量均呈正相关(r=0.849,0.848;均P<0.001);股骨内髁非承重面、股骨外髁承重面及非承重面、胫骨外髁关节软骨T2值与滑液中IL-1含量均无相关性(均P>0.05)。结论:股骨内髁承重面及胫骨内髁关节软骨T2值与滑液中IL-1含量呈正相关,T2-mapping成像可作预测早期骨性关节炎炎症程度及活动期的一种无创性检查方法。

关键词： 药物性肝损伤   多烯磷脂酰胆碱   水飞蓟素   细胞因子   超氧化物歧化酶   治疗  

摘要： 目的回顾性分析观察应用多烯磷脂酰胆碱联合水飞蓟素胶囊治疗药物性肝损伤(DILI)患者的疗效。方法2021年1月~2024年7月我院诊治的DILI患者102例,被分为两组,每组51例。给予对照组多烯磷脂酰胆碱静脉滴注治疗,观察组在多烯磷脂酰胆碱静脉滴注治疗的基础上联合应用水飞蓟素胶囊口服,治疗2~4周。采用硫代巴比妥酸法和黄嘌呤氧化法检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH),采用ELISA法检测血清C-反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和IL-1β。结果经2~4周治疗,本组DILI患者肝功能复常率为91.2%,9例(8.8%)肝功能指标未正常;治疗后,观察组血清ALT、AST和ALP水平分别为(33.6±6.7)U/L、(35.8±7.1)U/L和(62.3±11.4)U/L,显著低于对照组【分别为(47.9±7.4)U/L、(52.8±7.0)U/L和(73.1±11.9)U/L,P<0.05】;观察组血清SOD和GSH水平分别为86.0(74.6,93.2)U/L和(7.9±1.3)μmol/L,显著高于对照组【分别为73.5(69.1,81.2)U/L和(6.3±1.0)μmol/L,P<0.05】,而血清MDA水平为(5.2±1.1)nmol/L,显著低于对照组【(6.4±1.2)nmol/L,P<0.05】;观察组血清TNF-α和IL-1β水平分别为(4.6±1.8)pg/mL和(11.3±4.4)ng/mL,显著低于对照组【分别为(9.3±2.6)pg/mL和(26.4±6.7)ng/mL,P<0.05】。结论联用多烯磷脂酰胆碱和水飞蓟素胶囊治疗DILI患者可以获得肝功能指标恢复正常,可能与抑制氧化应激状态和减少细胞因子释放有关,值得临床继续观察。

关键词： mitochondrial thiamin pyrophosphate uptake   NF-κB signaling pathway   pancreatic acinar cells   proinflammatory cytokines   SLC25A19  

摘要： We investigated the effects of proinflammatory cytokines on carrier-mediated thiamin pyrophosphate (TPP) uptake by pancreatic acinar cells (PACs) mitochondria using mouse-derived pancreatic acinar 266-6 cells (PAC 266-6) and human primary PACs as models. First, we assessed the level of expression of the mitochondrial TPP transporter (MTPPT) mRNA in the pancreatic tissue of patients with chronic pancreatitis and found the level to be significantly lower than that in normal control subjects. We then examined the effects of exposing PACs to IL-1b, IL-6, and TNF-a on mitochondrial TPP uptake and observed significant inhibition by all these proinflammatory cytokines. Focusing on IL-1b (since it showed a more severe effect), we found this proinflammatory cytokine to also cause a significant inhibition in MTPPT protein and mRNA expression, as well as in the activity of the SLC25A19 promoter. Effect on the latter, appeared to be mediated via a decrease in the binding affinity of NF-Y (a nuclear factor that drives Slc25a19 promoter activity) as well as via epigenetic mechanism/histone-modification were significant reduction in levels of enrichment of the activator markers H3K4-trimethylation and H3K9-acetylation, and an increase in level of enrichment of the repressor marker H3K27-trimethylation were observed. Finally, evidence was obtained suggesting a role for the intracellular NF-κB signaling pathway in mediating the effects of IL-1b on PAC mitochondrial TPP uptake process. These results show that exposure of PACs to IL-1b causes inhibition in mitochondrial TPP uptake, and that this effect is exerted at the level of SLC25A19 transcription and is mediated via the NF-κB signaling pathway. Copyright © 2025 The Authors.

关键词： 呼吸道合胞病毒   融合前F蛋白   单克隆抗体   双抗体夹心ELISA   抗原定量  

摘要： 目的建立一种基于酶联免疫吸附技术(ELISA)并能简便、快速对呼吸道合胞病毒(RSV)融合前F蛋白(preF)进行定量检测的方法。方法利用HEK293悬浮细胞表达7种针对RSV F蛋白不同表位的单克隆抗体(D25、palivizumab、101F、MED18897、AM22、AM14、motavizumab)及preF、融合后F蛋白(postF)。根据7种单克隆抗体对preF、post F结合能力的不同,将可同时识别preF、post F抗体作为包被抗体,特异性识别preF抗体作为酶标抗体,选择信噪比最大的组别建立针对preF的双抗体夹心ELISA检测及定量方法。结果本研究建立的以motavizumab作为包被抗体,D25作为酶标抗体的双抗体夹心ELISA方法可特异性识别RSV preF并对其定量,并且该方法具有良好的特异性和灵敏度,其检测灵敏度为7.8 ng/mL、线性范围为7.8 ng/mL~0.5μg/m L,r^(2)为0.9932,同一样本3次重复检测结果的CV值为0.15%~6.86%。结论motavizumab作为包被抗体,D25作为酶标抗体的双抗体夹心ELISA方法特异性好、灵敏度高、稳定性佳,可用于RSV疫苗产品及前期研究样品中preF抗原含量测定,为RSV疫苗的研制及工艺开发奠定基础。