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关键词： 结核性胸水   肿瘤性胸水   结核抗体   腺苷脱氨酶   糖类抗原125  

摘要： 目的 探讨结核抗体(tuberculosis antibody,TB-Ab)、腺苷脱氨酶(adenosine deaminase,ADA)和糖类抗原125(carbohydrate antigen 125,CA125)单项及联合检测在鉴别结核性和肿瘤性胸水中的应用价值。方法 此项为回顾性研究,纳入2020年1月至2024年6月初诊为结核性胸水的患者75例为观察组,以同期因“胸腔积液”住院且诊断为非结核性胸水的患者75例为对照组。采用受试者工作特征(ROC)曲线分析比较TB-Ab、ADA、CA125单独及联合检测在诊断结核性胸膜炎中的效能。结果 ①观察组患者的TB-Ab阳性率显著高于对照组(60.00%对9.33%,P<0.001);观察组患者的ADA[(43.44±15.51) U/L]和CA125[(175.57±64.66) U/mL]均显著高于对照组[(27.81±3.42) U/L]和[(122.35±41.22) U/mL],P<0.001;②ADA以34 U/L为切点时,表现出较高的诊断效能,AUC为0.856,灵敏度和特异度分别为98.67%和78.67%;CA125以180 U/mL为切点时,虽然灵敏度较高(89.94%),但特异度较低(52.33%),AUC为0.739,单独使用效果有限;TB-Ab的AUC为0.753,灵敏度为90.67%,特异度为61.22%。③三者联合检测的AUC显著提升至0.926,灵敏度和特异度分别达到90.67%和86.67%。结论 TB-Ab、ADA和CA125联合检测能够更准确地诊断结核性胸膜炎,联合检测的诊断效能优于单项检测。

摘要：

关键词： AMR   antibody mediated rejection   DSA   donor specific antibody   HLA   human leukocyte antigens   ISHLT   International Society of Heart and Lung Transplantation   dd-cfDNA   donor-derived cell-free DNA   CLAD   chronic lung allograft dysfunction   HR   hazard ratio   SNP   single nucleotide polymorphism   MFI   mean fluorescent intensity  

摘要： Antibody-mediated rejection (AMR) of the lung is one of the great diagnostic and therapeutic challenges in our field. Donor specific antibodies (DSA) against human leukocyte antigens (HLA) have been shown to increase the risk of lung graft failure...

关键词： 结石性胆囊炎   急性   腹腔镜胆囊切除术   腹壁穿刺拉钩三孔法   前白蛋白   C反应蛋白   白细胞介素-8   脂肪酶   手术后并发症  

摘要： 目的 探讨腹壁穿刺拉钩三孔法腹腔镜胆囊切除术(LC)对急性结石性胆囊炎患者治疗效果及血清前白蛋白(PA)、C反应蛋白(CRP)、白细胞介素-8(IL-8)的影响。方法 选取2022年6月至2024年5月收治的急性结石性胆囊炎患者146例,根据手术方法不同分为三孔法组75例和传统组71例,三孔法组采用腹壁穿刺拉钩三孔法LC治疗,传统组采用传统四孔法LC治疗。比较两组患者手术指标和住院时间,术前及术后12、24 h疼痛视觉模拟评分法(VAS)评分,术前、术后3 d患者血清PA、脂肪酶(LPS)及炎性因子[CRP、IL-8、肿瘤坏死因子-α(TNF-α)]水平,以及术后并发症发生率。结果 三孔法组患者手术时间、术中出血量和住院时间均少于或短于传统组患者(P<0.01)。术后12、24 h,三孔法组患者疼痛VAS评分均低于传统组患者(P<0.01)。术后3 d两组患者血清PA水平均升高,血清LPS水平均降低,且三孔法组患者血清PA水平高于传统组患者,血清LPS水平低于传统组患者(P<0.05,P<0.01)。术后3 d两组患者血清CRP、IL-8、TNF-α水平均降低,且三孔法组患者低于传统组患者(P<0.05,P<0.01)。三孔法组患者术后并发症总发生率低于传统组患者(P<0.05)。结论 腹壁穿刺拉钩三孔法LC能够有效促进急性结石性胆囊炎患者术后恢复,减轻患者术后疼痛,改善患者炎症反应,且术后并发症发生率较低。

摘要： Purpose: T-cell stemness is important for antitumor immunity. The presence in tumor-draining lymph nodes and tumor of stem-like memory T (TSCM) cells and T cells expressing the transcription factor T-cell factor 1 (TCF1), critical in T-cell self-renewal, is associated with improved response to immune checkpoint *** Design: We studied the effects of the tumor-targeting immunocytokine PDS01ADC (NHS-IL12) on peripheral T-cell stemness in murine hosts and in patients with advanced solid tumors. TSCM and expression of the murine T-cell stemness markers stem cell antigen 1 (Ly6a) and Tcf7 were evaluated in na & iuml;ve and tumor-bearing mice receiving murine PDS01ADC (NHS-muIL12). Peripheral blood from patients treated in a clinical trial with PDS01ADC was analyzed for TSCM and expression of TCF1 on T-cell ***: NHS-muIL12 treatment in na & iuml;ve mice increased stem cell antigen 1-positive peripheral T cells with stem-like phenotypes and promoted tumor infiltration of CD8+ T cells displaying increased stemness. In patients with advanced solid tumors, PDS01ADC treatment increased peripheral CD8+ and CD4+ TSCM frequencies and effector memory T cells expressing TCF1, with greater increases associated with disease control. Most peripheral TSCM cells were negative for PD-1 and TIGIT throughout treatment, suggesting their quiescence and self-renewal capacity. Expanded TCF1-negative effector memory CD8+ T cells expressed PD-1 with increased intensity of granzyme B, indicating an activated, cytotoxic ***: PDS01ADC treatment in patients with advanced solid tumors boosts peripheral T cells with stem-like characteristics, correlating with disease stabilization. Further studies combining PDS01ADC with other immunotherapies to synergize with this peripheral burst of T-cell stemness are warranted.

关键词： HPV16 E6突变抗原肽   DC细胞   小鼠宫颈癌模型   治疗效果  

摘要： 目的:建立宫颈癌小鼠模型,探讨负载HPV16 E6抗原肽的树突状细胞(DC)的肿瘤抑制效果及免疫应答反应。方法:选取6~8周龄的健康C57BL/6雌性小鼠,通过皮下注射TC-1细胞的方法建立宫颈癌模型,待肿瘤长至15mm时,将小鼠平均分成3组:control组、DC组、DC+Peptide组。比较各组肿瘤面积,绘制肿瘤生长曲线。从小鼠的脾脏分离出淋巴细胞,流式细胞术检测各组Treg(CD4+CD25+Foxp3+)、IFN-γ表达情况。结果:DC+Peptide组肿瘤面积及重量均显著低于control组及DC组。小鼠脾组织中DC+Peptide组的CD4+IFN-γ、CD8+IFN-γ表达量显著高于control组、DC治疗组,差异有统计学意义(P<0.05);DC+Peptide组Treg数量显著低于control组及DC治疗组,差异有统计学意义(P<0.05)。结论:负载HPV16 E6突变抗原肽的DC细胞,能有效激活小鼠T淋巴细胞,引发特异性抗肿瘤免疫反应,从而抑制肿瘤生长。

关键词： Adalimumab   Cytokine   Etanercept   Infliximab   Paradoxical  

摘要：

The inhibition of tumor necrosis factor-α (TNF-α) signaling with inhibitors (TNFi) like infliximab, adalimumab, or etanercept has become a staple of dermatological and rheumatological therapy. Intriguingly, a sizeable minority of patients under TNFi therapy develop alopecia areata (AA), the most common inflammatory hair loss disease, at a higher prevalence than the general population. This appears paradoxical, since the pro-inflammatory Th1 cytokine TNF-α, inhibits hair follicle (HF) growth, but encourages one to reconsider the as yet under-investigated roles of TNF-α in human HF physiology. Because AA only affects HFs whose immune privilege (IP) has collapsed, the occurrence of AA under

Haplotype-resolved assemblies of the MHC region in five widely used tumor cell lines

Haozhe Yuan Mengping Jiang Xingyu Xu Jialiang Zhu Shulong Dong Weida Meng Dandan Zhang Jiakang Ma Yicheng Lin Ziqiang Chen Shaoyang Sun Wenqing Qiu Yun Liu 

MOE Key Laboratory of Metabolism and Molecular Medicine Department of Biochemistry and Molecular BiologySchool of Basic Medical Sciences and Shanghai Xuhui Central HospitalFudan UniversityShanghai 200032ChinaState Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai 200032ChinaMOE Key Laboratory of Metabolism and Molecular Medicine Department of Biochemistry and Molecular BiologySchool of Basic Medical SciencesFudan UniversityShanghai 200032ChinaShanghai Xuhui Central Hospital Shanghai 200032China

来源 维普期刊数据库 ScienceCitationIndex... PubMed期刊 详细信息

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• 维普期刊数据库
• ScienceCitationIndexExpanded
• PubMed期刊

关键词： Cell lines   CRISPR-Cas9   Haplotype-resolved assembly   MHC   Targeted sequencing  

摘要： The major histocompatibility complex(MHC)region plays a crucial role in immune function and is implicated in various diseases and cancer ***,its high polymorphism poses challenges for accurate genetic profiling using conventional reference ***,we present high-quality,haplotype-resolved assemblies of the MHC region in five widely used tumor cell lines:A549,HeLa,HepG2,K562,and *** oncological studies extensively employ these cell lines,ranging from basic molecular research to drug discovery and personalized medicine *** integrating CRISPR-based targeted enrichment with 10×Genomics linked-read and PacBio HiFi long-read sequencing,we constructed MHC haplotypes for each cell line,providing a valuable resource for the research *** these assembled haplotypes as references,we characterize the aneuploidy of the MHC region in these cell lines,offering insights into the genetic landscape of this critical immunological *** work addresses the urgent need for accurate MHC profiling in these widely used cell line models,enabling more precise interpretation of existing and future genomic and epigenomic *** resource is expected to significantly enhance our understanding of tumor biology,immune responses,and the development of targeted therapies.

关键词： 猪繁殖与呼吸综合征病毒   非结构蛋白1α   原核表达   多克隆抗体   生物学特性  

摘要： 【目的】制备非结构蛋白1α(non-structural protein 1α,NSP1α)多克隆抗体,为深入研究NSP1α蛋白功能提供物质基础,并为猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)防控靶点的选择提供新思路。【方法】以pCAGGS-NSP1α-HA质粒为模板,聚合酶链式反应扩增NSP1α基因,利用相关生物信息学在线软件分析PRRSV SD16毒株NSP1α基因和其他PRRSV毒株NSP1α基因序列同源性、蛋白的理化性质、氨基酸亲-疏水性、跨膜结构域、信号肽序列、磷酸化位点、二级和三级结构。通过EcoR I和Xho I酶切位点将NSP1α克隆至pET-28a-His原核表达载体。测序正确的pET-28a-NSP1α-His质粒转化至大肠杆菌*** BL21(DE3)表达感受态细胞,将诱导表达、纯化复性后的NSP1α蛋白免疫小鼠制备多克隆抗体,Western blot和间接免疫荧光试验(indirect immunofluorescence assay,IFA)鉴定制备的多克隆抗体的反应原性,通过Western blot和激光共聚焦分析NSP1α蛋白亚细胞分布。【结果】信息学分析结果表明,SD16毒株NSP1α蛋白有501个氨基酸,为不稳定的疏水性蛋白,其序列与HLJ毒株序列同源性最高,与I型PRRSV序列同源性最低;NSP1α蛋白无信号肽序列,无跨膜结构域,存在13个磷酸化位点;二级结构主要包含α-螺旋、延伸链和无规则卷曲。将获得的高纯度并且有活性的NSP1α蛋白进行小鼠免疫,检测其血清抗体效价可达1∶64000。Western blot和IFA结果显示,制备的多克隆抗体特异性识别PRRSV NSP1α蛋白。Western blot和激光共聚焦结果显示,NSP1α蛋白主要定位在细胞核内。【结论】成功表达、纯化了PRRSV NSP1α蛋白并制备了鼠源多克隆抗体,该多克隆抗体与PRRSV NSP1α蛋白具有良好的反应活性和特异性。