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关键词： 淋巴瘤   化疗   化毒祛邪汤   疗效   细胞免疫功能  

摘要： 目的 探讨化毒祛邪汤联合CHOP化疗方案治疗淋巴瘤的疗效及对细胞免疫功能的影响。方法 选择医院2020年1月-2023年12月所接诊的102例淋巴瘤患者作为研究对象,根据随机数表法将患者分为对照组(51例)、观察组(51例)。对照组采用CHOP化疗方案治疗,观察组在对照组基础上联合化毒祛邪汤治疗,4周为1个治疗周期,两组患者均接受4个周期的治疗。治疗4个周期后,比较两组的临床疗效,并比较治疗前、治疗4个周期后的细胞免疫功能(CD_(4)^(+)、CD_(8)^(+)、CD_(4)^(+)/CD_(8)^(+))、肿瘤标志物[血管内皮生长因子(VEGF)、胸腺嘧啶脱氧核苷激酶(TK-1)]变化,并记录治疗期间不良反应的发生情况。结果 观察组治疗后的总有效率明显高于对照组,差异有统计学意义(P<0.05);观察组治疗后的细胞免疫功能指标CD_(3)^(+)、CD_(4)^(+)及CD_(4)^(+)/CD_(8)^(+)水平均明显高于对照组,差异有统计学意义(P<0.05);观察组治疗后的肿瘤标志物血清VEGF、TK-1水平均明显低于对照组,差异有统计学意义(P<0.05);两组在治疗期间的不良反应总发生率比较,差异无统计学意义(P>0.05)。结论 化毒祛邪汤联合CHOP化疗方案治疗淋巴瘤的疗效显著,能提高细胞免疫功能,降低肿瘤标志物血清VEGF、TK-1水平表达,值得在临床中推广应用。

关键词： Mesenchymal stem cells   Cartilage   Small interfering RNA   Osteoarthritis   Knees   Histology   Transfection   Skeletal joints  

摘要： Background Knee osteoarthritis (OA) currently lacks disease-modifying treatments. While autologous mesenchymal stem cell (MSC) transplantation has shown promise, its limitations have led to growing interest in allogeneic MSC therapy. The aim of this study was to clarify the functional role of hepatocyte growth factor (HGF) in the chondroprotective effects of synovial MSCs transplanted under fully major histocompatibility complex (MHC)-mismatched allogeneic conditions in a rat model. Methods Synovial MSCs were isolated from the knees of ACI rats and transfected with either Hgf siRNA or control siRNA. HGF knockdown was confirmed at both the mRNA and protein levels. Chondrogenic capacity was assessed by evaluating the cartilage pellet size after in vitro differentiation. OA was surgically induced in Lewis rats by anterior cruciate ligament transection (ACLT). At one week post-surgery, the rats (n = 20 knees/group) received weekly intra-articular injections of one of the following for 7 weeks: (1) vehicle control (medium alone), (2) control siRNA-treated MSCs, or (3) Hgf siRNA-treated MSCs (1 × 10⁶ cells). At 8 weeks post-injection, cartilage degeneration was evaluated using macroscopic and histological Osteoarthritis Research Society International (OARSI) scoring. Results Transfection with Hgf siRNA reduced Hgf gene expression to 30% of the control levels and significantly decreased HGF protein secretion. HGF knockdown impaired in vitro chondrogenic differentiation, resulting in a reduced cartilage pellet size. In vivo, treatment with control siRNA-treated MSCs significantly delayed OA progression compared to the vehicle group, while Hgf siRNA-treated MSCs demonstrated diminished therapeutic efficacy, indicating reduced chondroprotective effects when HGF secretion was suppressed. Conclusions Synovial MSCs derived from ACI rats effectively delayed OA progression in Lewis rats despite the full MHC mismatch. HGF secretion played a critical role in mediating the

关键词： γ-谷氨酰转肽酶   自身免疫性胰腺炎   免疫球蛋白G4   诊断价值  

摘要： 目的探讨血清免疫球蛋白G4(IgG4)、γ-谷氨酰转肽酶(γ-GT)联合对自身免疫性胰腺炎(AIP)的鉴别诊断价值分析。方法选取2023年6月至2024年12月我院收治并确诊的AIP患者62例(AIP组)与胰腺癌患者62例(胰腺癌组),以及同期来院体检的健康者62例(健康组)。结果较胰腺癌组而言,AIP组IgG4、γ-GT水平更低,较健康组而言,AIP组IgG4、γ-GT水平更高(P<0.05)。较Ⅲ期患者而言,胰腺癌Ⅱ期患者IgG4、γ-GT水平更低,较Ⅰ期患者而言,Ⅱ期组IgG4、γ-GT水平更高(P<0.05)。多因素logistic回归分析显示IgG4、γ-GT水平是AIP发病影响因素(P<0.05)。血清IgG4、γ-GT水平在AIP中呈正相关(P<0.05);血清IgG4、γ-GT单独诊断及联合诊断AIP的曲线下面积(AUC)分别为0.908、0.820、0.926,敏感度、特异度分别为80.6%/90.3%、95.2%/58.1%、85.5%/88.7%。结论血清IgG4、γ-GT的检测对于AIP的诊断鉴别临床参考价值,且将IgG4、γ-GT联合应用有助于提高AIP的诊断效能。

关键词： alopecia areata   asthma   atopic dermatitis   dupilumab   IL-4/IL-13 signaling   IL-4R alpha subunit   Koebner phenomenon   melanocytes   monoclonal antibody   refractory pruritus   repigmentation   Th2 cytokines   vitiligo   AA   alopecia areata   AD   atopic dermatitis   AU   alopecia universalis   IL   interleukin  

摘要： The same patient featured in Fig 1 after 1 years of dupilumab usage. A 61-year-old African American female with atopic dermatitis (AD), alopecia areata (AA), and vitiligo before starting therapy with dupixent 600 mg loading dosage and 300 mg every...

关键词： 原发性免疫性血小板减少症   静注人免疫球蛋白   糖皮质激素   疗效   不良反应  

摘要： 目的 观察静注人免疫球蛋白联合糖皮质激素治疗儿童原发性免疫性血小板减少症(ITP)的临床疗效。方法 回顾性分析2022年9月至2024年12月河南科技大学第一附属医院收治的82例ITP患儿病历资料,根据不同治疗方案将患儿分为两组,各41例。对照组口服醋酸泼尼松片。研究组在对照组治疗基础上加用静注人免疫球蛋白(IVIg)治疗。两组治疗4周后判定疗效。对比两组血小板动态变化、不良反应发生率、免疫指标、炎症指标方面的差异性。结果 研究组治疗有效率高于对照组,差异有统计学意义(P<0.05)。开始治疗后24 h、48 h,研究组血小板早期应答率均高于同期对照组,差异有统计学意义(P<0.05);治疗期间研究组血小板峰值水平高于对照组,差异有统计学意义(P<0.05)。研究组不良反应发生率低于对照组,差异有统计学意义(P<0.05)。治疗4周后,研究组血清免疫球蛋白G(IgG)水平高于对照组,白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平低于对照组,差异有统计学意义(P<0.05)。结论 IVIg联合糖皮质激素治疗儿童ITP可提升临床疗效,改善血小板动态变化情况与免疫、炎症指标,同时降低不良反应发生率,具有临床应用意义。

关键词： HSP90   ADAM33   IL-lβ   支气管哮喘   治疗效果  

摘要： 目的:探讨血清热休克蛋白90(HSP90)、解整合素-金属蛋白酶33(ADAM33)、白细胞介素-1β(IL-1β)水平联合检测对支气管哮喘(简称哮喘)患儿治疗效果的评估价值。方法:选取2022年1月至2024年3月周口市中心医院177例哮喘患儿为研究对象,依据病情程度分为重度组(n=44)、中度组(n=61)、轻度组(n=72),另选取同期177例健康体检儿童为对照组。对比4组入院时血清HSP90、ADAM33、IL-lβ水平并分析其相关性,采用Logistic回归分析影响患儿治疗效果的单因素及多因素,并分析各指标水平联合检测对患儿治疗效果的预测价值。结果:4组入院时血清HSP90、ADAM33、IL-lβ水平比较,对照组<轻度组<中度组<重度组(P<0.05);入院时血清HSP90、ADAM33、IL-lβ水平均与患儿病情程度呈正相关(P<0.05);未有效控制患儿入院时动脉血二氧化碳分压(PaCO_(2))、HSP90、ADAM33、IL-lβ均高于有效控制、动脉血氧分压(PaO_(2))均低于有效控制患儿(P<0.05);PaCO_(2)、HSP90、ADAM33、IL-lβ水平为哮喘患儿未有效控制的危险因素,PaO_(2)为哮喘患儿未有效控制的保护因素(P<0.05);入院时血清HSP90、ADAM33、IL-lβ水平联合预测患儿未有效控制的效能为0.927,均高于各指标单独预测(P<0.05)。结论:哮喘患儿血清HSP90、ADAM33、IL-lβ水平与病情程度有关,其联合检测可有效预测患儿治疗效果,为早期临床合理治疗提供参考。

关键词： airway physiology   airway sympathetic nerves   allergic asthma   flexiVent   Muc5ac  

摘要： Excessive mucus in the airways is an underlying pathological feature of many airway diseases, including asthma. Therapeutic options to reduce mucus production in the airways remain limited. One possible therapeutic target is the airway sympathetic nerves. Although lung sympathetic innervation has been considered sparse, sympathetic nerves secrete neurotransmitters that act on adrenergic receptors, including β2-adrenergic receptor (β2AR). Interestingly, in experimental models, chronic use of β2AR agonists can augment mucus secretion. Thus, in the present study, we tested the hypothesis that airway sympathetic nerves regulate mucus production in the airway in response to the type 2 cytokine interleukin 13 (IL-13). We performed airway sympathectomy using intranasal instillation of the synthetic neurotoxin 6-hydroxydopamine (6-OHDA). Airway sympathectomy attenuated multiple IL-13-mediated airway deficits, including density of goblet cells containing neutral mucins, transcriptional ratio of mucin 5ac (Muc5ac) to mucin 5b (Muc5b), and airway elastance and tissue damping. Although total Muc5ac and Muc5b transcript levels and Muc5ac and Muc5b protein levels in bronchoalveolar lavage were not significantly altered, these changes suggest that airway sympathectomy modifies goblet cell phenotype and mucin composition. Airway sympathectomy also dampened IL-13-mediated increases in total lung transcripts important for regulating allergic responses, including interleukin 6, complement component 3, and colony-stimulating factor. This study reveals that airway sympathetic nerves regulate physiologic, molecular, and inflammatory responses to type 2 (IL-13-mediated) airway inflammation and raises the possibility that they may serve as potential targets for therapeutic *** & NOTEWORTHY The role of airway sympathetic nerves in regulating airway responses remains largely undefined. We demonstrated that chemical depletion of airway sympathetic nerves attenuates specific IL-13

关键词： 糖尿病足溃疡   中医外治法   细胞因子  

摘要： 糖尿病足溃疡(diabetic foot ulcer,DFU)作为糖尿病的一种严重并发症,其发病率随着社会老龄化的进程不断攀升。正常创面的愈合是一个复杂的过程,包括炎症阶段、增殖阶段和组织重塑阶段,这3个阶段相互重叠,共同促进伤口的愈合。然而,糖尿病足溃疡创面在愈合过程中的表现却常常显得异常。近年来,中医外治法在治疗DFU方面取得了显著的疗效,尤其在局部创面处理上表现出了显著的优势。然而,尽管中医外治法在临床实践中表现出色,但其具体的作用机制及作用靶点却尚未完全明确。细胞因子如白细胞介素、干扰素、生长因子等在创面愈合过程中发挥着重要作用。中医外治法通过调节如AGEs/RAGE、NF-κB、PI3K/Akt等信号通路,影响相关细胞因子的表达,从而干预不同的愈合阶段,促进糖尿病创面的愈合。因此,深入探究中医外治法修复DFU创面的作用机制及靶点,不仅有助于揭示中医外治法的科学内涵,也有助于推动其在临床上的进一步推广和应用。文章将从细胞因子的角度,对中医外治法修复DFU创面的相关研究进行回顾和总结。

关键词： 艾灸   神经炎症   海马   肾脑相关  

摘要： 目的观察艾灸“百会”“涌泉”对阿尔茨海默病(AD)小鼠学习记忆能力、海马炎性细胞因子表达的影响,并基于“肾脑相关”理论探讨其作用机制。方法24只APP/PS1小鼠随机分为AD模型组、艾灸组,10只同窝野生C57小鼠设为空白对照组。艾灸选穴“百会”“涌泉”,5 d为一个疗程,共治疗4个疗程。治疗结束后,采用Morris水迷宫检测各组小鼠空间学习记忆能力;免疫荧光检测皮层和海马神经元(NEUN),以及海马白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α的表达;ELISA检测海马组织IL-1β、TNF-α的含量。结果与空白对照组比较,AD模型组小鼠逃避潜伏期、平均游泳距离显著增加(P<0.001),穿越平台次数减少(P<0.001);皮层和海马神经元NEUN阳性表达面积百分比显著减少(P<0.01),海马IL-1β、TNF-α表达增多(P<0.05);海马IL-1β、TNF-α含量均增加,但差异无统计学意义。与AD模型组比较,艾灸组小鼠(定位航行)逃避潜伏期明显缩短(P<0.001),平均游泳距离减少、穿越平台次数增加,但差异未见统计学意义;皮层、海马神经元NEUN阳性表达面积百分比显著增加(P<0.01),海马IL-1β表达显著减少(P<0.01),TNF-α表达下降,但差异未见统计学意义;海马IL-1β含量显著降低(P<0.05),TNF-α含量有降低趋势,但差异不具有统计学意义。结论艾灸“百会”“涌泉”可降低海马炎性细胞因子的阳性表达和组织含量,保护皮层与海马神经元,进而改善AD小鼠空间学习记忆能力。

摘要： Objective: T cell receptor αβ⁺ (TCRαβ⁺) CD4⁻ CD8⁻ double-negative (DN) T cells are expanded in lupus-prone mice and patients with systemic lupus erythematosus (SLE), produce interleukin-17 (IL-17), and contribute to disease pathogenesis. However, it is not known whether there is functional heterogeneity within this population. This study aimed to determine whether a subset of DN T cells produces IL-10 and whether the ratio of IL-17⁺/IL-10⁺ DN T cells correlates with disease activity. Methods: Flow cytometry was used to analyze DN T cells in lupus-prone MRL/lpr mice and patients with SLE. IL-17⁺ and IL-10⁺ DN T cells were identified and quantified in the peripheral blood and lymphoid organs. Correlations between the IL-17⁺/IL-10⁺ DN T cell ratio and clinical parameters, including the SLE Disease Activity Index (SLEDAI) and proteinuria, were examined. Results: IL-17⁺ DN T cells were increased in lupus-prone mice, whereas IL-10⁺ DN T cells decreased in aging MRL/lpr mice. In patients with SLE (n = 67), the IL-17⁺/IL-10⁺ DN T cell ratio was associated with higher SLEDAI scores and was elevated in those with proteinuria. A longitudinal analysis of patients with SLE similarly showed a positive correlation between SLEDAI scores and the IL-17⁺/IL-10⁺ DN T cell ratio. Conclusion: The observation of nonoverlapping IL-10⁺ and IL-17⁺ DN T cells suggests heterogeneity within the DN T cells in both lupus-prone mice and patients. The IL-17⁺/IL-10⁺ DN T cell ratio may serve as a biomarker to monitor disease activity in patients with SLE. © 2025 The Author(s). ACR Open Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology.