课程文献中心 更多>>

关键词： Disease-modifying Drugs (DMARDs)   Cytokines and Chemokines   Targeted synthetic drugs   Adaptive immunity  

摘要： [This corrects the article DOI: 10.1007/s10616-024-00698-z.].

关键词： IL-10   Colorectal cancer liver metastasis   Breast cancer liver metastasis   Other cancer liver metastasis   Research progress  

摘要： Liver metastasis can occur in a wide range of cancers and have a significant impact on patient survival and prognosis. Once liver metastasis occurs, patients often lose the opportunity for surgery, and although a small percentage of patients can undergo hepatic resection to prolong survival, the benefit is not great. There were also many factors affecting liver metastasis, including reprogramming of the primary tumor metabolism, disturbances in the immune microenvironment and immune cells, alterations in the gut microbiota, and epigenetic changes. Interleukin-10 (IL-10) has a dual role as a cytokine that has been found in recent years to be pro-inflammatory as well as pro-liver metastasis. IL-10 exerts pro-metastatic effects mainly by regulating the polarization of tumor macrophages in the tumor microenvironment, especially by promoting the polarization of M2 macrophages. However, the role of IL-10 in tumorigenesis and progression remains controversial and the molecular mechanism involved in promoting liver metastasis is currently unclear. In view of the increasing role of IL-10 in promoting liver metastasis, this review summarizes the role of IL-10 in liver metastasis of colorectal cancer, breast cancer and other tumors in recent years, and provides ideas for subsequent clinical practice and basic research.

关键词： Extracellular vesicles   Nanoparticles   Chlorophyll   Euglena gracilis   Anti-inflammation  

摘要： The development of materials from the photosynthetic microorganism Euglena (E. gracilis, as represented in this study) has gained attention as a new source of biofuel. In addition, Euglena is being marketed as a new food product, with the anti-inflammatory effects of the polysaccharide beta-1,3-glucan highlighted as one of its features. Recent advances in studies on extracellular vesicles (EVs), not only in mammals but also in plants and bacteria, have prompted us to explore Euglena extracellular vesicle-like nanoparticles (eEV-LNPs) as new anti-inflammatory agents. Although the amount of eEV-LNPs in the medium is low, it significantly increases after the heat denaturation of whole Euglena cultures at 120 degrees C for 20 min. This increase correlates with enhanced anti-inflammatory activity, as demonstrated by assays using RAW264.7 cells, particularly evident in the suppression of interleukin-6 (IL-6) expression to 10% of induced level by lipopolysaccharide. The administration of the water-soluble fraction from the heat-denatured Euglena culture to mice with a dextran sodium sulfate-induced colitis model ameliorates inflammatory symptoms by reducing IL-6 expression. Interestingly, the eEV-LNPs exhibited autofluorescence. Chloroform/methanol extraction of Euglena cells suggested that the amount of 7-hydroxymethyl pheophytin, a chlorophyll derivative with fluorescence, increases under 120 degrees C treatment and shows affinity for milk exosomes and anti-inflammatory activities with the suppression of IL-6 expression to 20% of induced level by lipopolysaccharide. These results suggest that heat-treated Euglena cells artificially produce autofluorescent eEV-LNPs encapsulating anti-inflammatory activity, which could serve as novel nanomaterials originated from Euglena.

关键词： anti-TNF-alpha agents   granulocyte and monocyte adsorptive apheresis (GMA)   loss of response   maintenance therapy   ulcerative colitis  

摘要： Ulcerative colitis (UC) is a chronic inflammatory condition requiring lifelong management, with anti-tumor necrosis factor alpha (anti-TNF-alpha) agents often used for refractory cases. However, secondary loss of response (LOR) to these agents, due to anti-drug antibodies, poses a significant therapeutic challenge. This report describes a case where granulocyte and monocyte adsorptive apheresis (GMA) maintenance therapy successfully restored the efficacy of an anti-TNF-alpha agent in a 26-year-old male with active UC experiencing LOR to infliximab. Following GMA induction therapy and continued infliximab administration, clinical symptoms improved, fecal calprotectin levels decreased, and clinical remission was achieved. Long-term maintenance with GMA enabled sustained clinical remission, with mucosal healing observed one year post-therapy. This case suggests that GMA maintenance therapy may serve as a novel therapeutic approach for patients with active UC experiencing LOR to anti-TNF-alpha agents. However, further studies are warranted to elucidate the underlying mechanisms and validate its efficacy.

摘要： Purpose (the aim of the study): Exploring the effects and potential biological mechanisms of the inflammatory factor il-1β on the biology and cell mechanics of superficial chondrocytes Methods: In this study, human cartilage tissues were firstly o...

关键词： Interleukin-35   Interleukin-37   Inflammation   Parkinson's disease  

摘要： Background: Parkinson's disease (PD) is increasingly recognized as a condition driven by both central and peripheral inflammatory responses, largely mediated by cytokine activity. Objective: To assess IL-35 (P35 and Ebi3 subunits) and IL-37 gene expression, along with the serum levels of IL-35 protein in patients with PD compared to healthy controls. Methods: Cytokine gene expression was measured using the qRT-PCR technique, while IL-35 serum levels were measured using the ELISA method. The data obtained were analyzed using a Bayesian regression model in the R software. Results: The results revealed that the expression of P35 gene, of the two subunits of IL-35, did not differ significantly between the two groups. However, Ebi3 and IL-37 transcript levels were significantly lower in patients compared to healthy individuals (p<0.001). In contrast, IL-35 serum level in patients showed a significant increase compared to the control group (p=0.016). Notably, IL-37 expression showed a negative correlation with age (p=0.004). We also observed positive and significant correlations between the gene expression of P35 and Ebi3 (p=0.02, r=0.4), P35 and IL-37 (p=0.008, r=0.45), and Ebi3 and IL-37 (p=0.016, r=0.41). Conclusion: In conclusion, our study revealed a higher serum protein level of IL-35 in PD patients compared to the healthy control group. Meanwhile, gene expression levels of IL-37 and Ebi-3 were significantly reduced. These alterations in the expression of these cytokines are suggested to be partly responsible for the immune system dysregulation in this disease.

关键词： Heart Failure   Transverse aortic constricition   Interleukin-22   Inflammation   Fibrosis  

摘要： Heart failure (HF) due to left ventricular (LV) dysfunction remains a major global health challenge, with inflammation driving its progression under chronic pressure overload, such as hypertension. This study explored the role of interleukin-22 (IL-22), a cytokine associated with tissue protection, in HF induced by transverse aortic constriction (TAC). IL-22 knockout (KO) mice exhibited exacerbated HF, marked by worsened LV hypertrophy, heightened inflammation, and impaired cardiac function compared to wild-type controls. Conversely, treatment with recombinant IL-22Fc improved LV function, reduced inflammatory cell infiltration, and alleviated cardiac remodeling and inflammation. These findings demonstrate that IL-22 plays a critical role in regulating inflammation and cardiac remodeling in pressure overload-induced HF. Targeting IL-22 may represent a promising therapeutic strategy to alleviate HF progression and associated pulmonary complications.

关键词： Bruton's tyrosine kinase inhibitor   diffuse large B-cell lymphoma   IL-10   natural killer group 2 member D   phosphorylated STAT3  

摘要： The aim of this study is to explore the role of the IL-10/STAT3 pathway in the upregulation of natural killer group 2, member D (NKG2D) ligands (MICA and ULBP2) induced by Bruton's tyrosine kinase (BTK) inhibitors in non-germinal center B-cell-like diffuse large B-cell lymphoma cells. The expression levels of NKG2D ligands and the IL-10/STAT3 pathway in SUDHL4, U2932, and OCI-LY3 cells were analyzed using western blotting. After stimulation of the B-cell receptor signaling pathway with IgM antibodies, the expression levels of NKG2D ligands, as well as IL-10 and phosphorylated STAT3 (p-STAT3) were significantly reduced. In contrast, treatment with ibrutinib produced effects opposite to those induced by IgM antibodies. Additionally, treatment of U2932 and OCI-LY3 cells with the STAT3 inhibitor (STAT3-IN-1) led to an increase in NKG2D ligand expression and a decrease in IL-10 levels. When IL-10 neutralizing antibodies were introduced, p-STAT3 levels decreased, and NKG2D ligand expression increased. Similar outcomes were observed when the BTK inhibitors ACP-196 and BGB-3111 were administered. Our findings suggest that the IL-10/STAT3 pathway plays a key role in the upregulation of NKG2D ligands induced by BTK inhibitors in U2932 and OCI-LY3 cells.

关键词： Histocompatibility   Immunologic desensitization   MHC class II   Organ transplantation  

摘要： Objectives: The functionality of the transplanted graft is considered the most important step in transplant. Both HLA tissue compatibility between recipients and donors and the presence of antibodies against the HLA structure in the recipient's serum play essential roles. The presence of these antibodies can be ascertained either before or after transplant. Although desensitization treatment provides the chance of transplant to immunized patients, it also prolongs graft survival in antibody-mediated rejection that develops after transplant. In this retrospective study, we aimed to determine treatment-related responses of anti-HLA antibodies in heart and renal transplant patients diagnosed with antibody-mediated rejection. Materials and Methods: We evaluated 60 samples from 18 patients who were followed in our center for at least 4 years and who had treatment before and after desensitization (plasmapheresis, intravenous immunoglobulin, and/or rituximab). Serum samples of the patients were tested for panel reactive antibody screening class I and II (One Lambda, Inc) test by Luminex method and single antigen class I and/or II (One Lambda, Inc) results by Luminex method. Mean fluorescence intensity >= 1000 was considered positive. To determine the presence of donor-specific antibodies, HLA tissue group samples of donors were studied by high-resolution method. Results: Studied antibodies, particularly those with mean fluorescence intensity >10 000 against class II HLA-DQ, remained resistant to treatment. Conclusions: Our study results showed that the class II HLA-DQ tissue group and anti-HLA antibodies against it play an important role in reducing the need for desensitization therapy and maintaining graft tissue functionality.