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关键词： AC005165.1   Apoptosis   Inflammation   miR-199a-3p   TXNIP  

摘要： Osteoarthritis (OA) is a chronic musculoskeletal disease and often causes impaired joint mobility and disability. Long noncoding RNAs (lncRNAs) play pivotal roles in OA development. This study was done to explore the role and mechanism of the lncRNA AC005165.1 in the cell model of interleukin-1 beta (IL)-1 beta-treated chondrocytes. This study recruited 20 surgically treated OA patients and 12 age- and gender-matched controls. Real-time reverse transcription quantitative polymerase chain reaction was used to examine the expression levels of AC005165.1, miR-199a-3p, and thioredoxin-interacting protein (TXNIP) in articular cartilage of patients and IL-1 beta-treated human chondrocytes. Cell viability and apoptosis were evaluated by cell counting kit-8 and flow cytometry assays, respectively. The protein levels of inflammatory cytokines were assessed by western blotting. Enzyme-linked immunosorbent assay was conducted to detect the concentrations of the inflammatory cytokines in chondrocytes. Luciferase reporter assay and Pearson's correlation analysis were used for analyzing the interaction and the correlation among AC005165.1, miR-199a-3p, and TXNIP. AC005165.1 expression was downregulated in cartilage of OA patients and chondrocytes treated with IL-1 beta, compared to that in the control groups. AC005165.1 knockdown increased apoptosis and aggravated inflammatory response in IL-1 beta-treated chondrocytes. AC005165.1 interacted with miR-199a-3p, and TXNIP was targeted by miR-199a-3p. In rescue assay, miR-199a-3p knockdown and TXNIP overexpression significantly reduced apoptosis and mitigated inflammatory response in IL-1 beta-treated chondrocytes with AC005165.1 knockdown. AC005165.1 knockdown promoted apoptosis and inflammatory response in IL-1 beta-treated chondrocytes via the miR-199a-3p/TXNIP axis.

关键词： HLA   HLA-A*01:470   novel allele   sequencing-based typing  

摘要： HLA-A*01:470 differs from HLA-A*01:01:01:01 by one nucleotide substitution in codon 119 in exon 3.

关键词： HLA   HLA-A*02:924   novel allele   sequence-based typing   Taiwanese  

摘要： One nucleotide mutation in exon 4 of HLA-A*02:07:01:01 results in the novel allele, HLA-A*02:924.

关键词： HLA-A*01:01:01:121   HLA-genotyping   Illumina   next generation sequencing   novel allele  

摘要： The HLA-A*01:01:01:121 allele differs from HLA-A*01:01:01:01 by a single nucleotide substitution in intron 4.

关键词： extremal combinatorics   graph cuts   graph theory  

摘要： Answering a question of Erd & odblac;s and Ne & scaron;et & rcaron;il, we show that the maximum number of inclusion-wise minimal vertex cuts in a graph on n $n$ vertices is at most 1.889 9 n $1.889{9}<^>{n}$ for large enough n $n$.

关键词： Alveolar bone   Anti-inflammatory   Flavonoid   Forastero cacao bean (Theobroma cacao L.) extract gel   NF- KB   Orthodontic tooth movement  

摘要： Background: Malocclusion can be corrected with orthodontic treatment to improve function and aesthetics. Orthodontic treatment patients have the potential to produce greater Reactive Oxygen Species (ROS), as evidenced by an increase in the Oxidative Status Index (OSI) a week after treatment. As a result, oxidative stress may induce an imbalance of bone remodeling through intensifying osteoclastogenesis by expressing pro- inflammatory cytokine, namely Interleukin-6 (IL-6). The worst pathological condition that can occur is Orthodontic-Induced Root Resorption (OIRR), a resorptive area on the root that is unwanted from orthodontic treatment. OIRR incidence is reported to be more than 90 % asymptomatic. Using Forastero cacao bean (Theobroma cacao L.) extract gel as a natural antioxidant and anti-inflammatory compound is expected to be able to prevent excess ROS, which triggers an increase in various pro-inflammatory cytokines, especially IL-6, which plays a role in the bone resorption pathway, thus reducing the incidence of OIRR and making orthodontic treatment successful through balanced bone remodeling. Aim: This study aimed to investigate the effect of Forastero cacao bean (Theobroma cacao L.) extract gel on the decrease of IL-6 cytokine expressed by osteoblasts, osteoclasts, and osteocytes in the OIRR model of male Wistar rats. Results: IL-6 positive osteoblasts, osteoclasts, and osteocytes decreased significantly after 8 % Forastero cacao bean extract gel-treated, as seen in T7 and T14 groups compared to C-7 and C-14 groups (p < 0.05). Conclusions: 8 % Forastero cacao bean extract gel decreased IL-6-positive osteoblasts, osteoclasts, and osteocytes in the tension side of the alveolar bone.

关键词： Skin infections   Skin tissue   Fibroblasts   Fungal diseases   Macrophages   T helper cells   Keratinocytes   Fungal pathogens  

摘要： Candida auris is an emerging multidrug-resistant skin-tropic fungal pathogen that causes serious human infections. However, the factors that regulate C. auris skin infection in vivo are still unclear. In this study, we identified that, unlike Candida albicans, which induces IL-17-secreting protective effector Th17 cells, C. auris predominately induces IFN gamma-secreting pathogenic Th1 cells during reinfection. Surprisingly, we found that IFN gamma enhances skin infection of C. auris but not C. albicans. Mechanistically, IFN gamma enhances skin infection of C. auris by dampening the protective IL-17 responses and increasing dermal damage. Furthermore, we identified that the development of Th1 cells occurs through IL-12, produced by C. auris-induced inflammatory macrophages and monocyte-derived dendritic cells. In addition, our findings reveal that C. auris unique cell wall outer mannan layer regulates the development of Th1 and Th17 cells. Collectively, our findings, for the first time, identified that C. auris induces IFN gamma to persist in the skin. These findings help explain why C. auris but not C. albicans preferentially persist in the skin long-term, with the potential to identify novel therapeutic approaches to prevent and treat this emerging fungal pathogen in humans.

关键词： B*27:247   HLA   sequence-based typing   Taiwanese  

摘要： One nucleotide substitution in codon -1 of HLA-B*27:04:01 results in a novel allele, HLA-B*27:247.

关键词： low back pain   lateral lumbar interbody fusion   indirect decompression   patient-reported outcome measures   Japanese orthopedic association back pain evaluation questionnaire   interleukin- 6  

摘要： Study Design A prospective *** This study aims to explore the correlation between interleukin (IL)- 6 levels in intervertebral disc (IVD) tissue and clinical outcomes in patients undergoing lumbar surgery for lumbar degenerative disease (LDD).Methods This prospective study analyzed 32 patients (22 men and 10 women, average age 69.6 years) who underwent lateral lumbar interbody fusion (LLIF). IL-6 gene expression in IVD tissues collected during surgery was measured and correlated with pre- and postoperative clinical outcomes, including pain intensity assessed via Numeric Rating Scales (NRS) and quality of life (QOL) evaluated through the Japanese Orthopaedic Association Back Pain Evaluation Questionnaire (JOABPEQ).Results IL-6 levels showed statistical correlations with postoperative intensity of low back pain (LBP) and several JOABPEQ domains. Patients with higher expression of IL-6 levels experienced more severe postoperative LBP and lower scores in lumbar function, walking ability, social life function, and mental health. The effectiveness rate of JOABPEQ scores was exceptionally high for low back pain (.548), walking ability (.677), and social functioning (.563), demonstrating the effectiveness of LLIF. The average operation time was 105.6 minutes, and the estimated blood loss was 85.6 *** The study underscores IL-6 as a potential biomarker for predicting surgical outcomes in LDD. High IL-6 levels correlate with worse postoperative LBP and lower QOL scores. Integrating molecular markers like IL-6 with patient-reported outcomes could provide a more comprehensive approach to postoperative care in spinal disorders, aiming to improve the overall QOL for LDD patients undergoing LLIF surgery.