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关键词： 呼吸系统疾病   非特异性   儿童   呼出气一氧化氮   肺功能   免疫球蛋白E   诊断效能  

摘要： 目的探讨呼出气一氧化氮(FeNO)和肺功能检测联合血清免疫球蛋白E(IgE)对儿童非特异性呼吸系统疾病的诊断效能。方法选取2021年9月~2022年9月于辽宁省健康产业集团本钢总医院儿科就诊的非特异性呼吸系统疾病患儿108例为研究组,另选取同时期的50例进行体检的健康儿童为对照组。检测两组的FeNO、肺功能指标[包括用力肺活量/预计值(FVC%pred)、最大用力呼气峰流量/预计值(PEF%pred)、一秒用力呼气容积/预计值(FEV_(1)%pred)、第一秒用力呼气容积/用力肺活量(FEV_(1)/FVC)]、血清IgE水平,并比较两组间的差异,采用受试者工作特征曲线(ROC)分析上述指标对儿童非特异性呼吸系统疾病的诊断效能。结果研究组的FeNO为(46.03±12.79)ppb,明显高于对照组的(13.51±6.22)ppb,差异有统计学意义(P<0.05);研究组的FVC%pred、PEF%pred、FEV_(1)%pred和FEV_(1)/FVC分别为(80.16±9.57)%、(70.60±9.65)%、(66.97±13.21)%、(73.59±13.72)%,均明显低于对照组的(97.44±9.02)%、(94.26±9.04)%、(93.91±10.73)%、(92.42±10.02)%,差异有统计学意义(P<0.05);研究组的血清IgE水平为(70.47±18.50)mIU/ml,明显高于对照组的(25.32±10.41)mIU/ml,差异有统计学意义(P<0.05);FeNO和血清IgE均为儿童非特异性呼吸系统疾病的危险因素,而FVC%pred、PEF%pred、FEV_(1)%pred和FEV_(1)/FVC是保护因素(P<0.05);ROC分析得出FeNO的AUC和特异度最高,分别为0.973和98.10%,其余指标的AUC为IgE(0.971)>PEF%pred(0.905)>FEV_(1)%pred(0.893)>FVC%pred(0.801)>FEV_(1)/FVC(0.775),其中IgE灵敏度最高,为96.00%;联合检测后的AUC为0.991,灵敏度和特异度为98.00%和98.10%。结论FeNO和肺功能联合血清IgE检测可以全面评估儿童的肺功能状况、气道炎症水平和过敏反应情况,为儿童非特异性呼吸系统疾病的精准诊断提供参考。

关键词： 胸部CT征象   乳酸脱氢酶   白介素-6   难治性肺炎支原体肺炎   诊断  

摘要： 目的:探讨胸部CT征象结合乳酸脱氢酶(lactate dehydrogenase,LDH)、白介素-6(interleukin-6,IL-6)检测对难治性肺炎支原体肺炎的诊断价值。方法:回顾性选取2023年1月-2024年5月徐州市妇幼保健院的72例难治性肺炎支原体肺炎患儿作为研究对象纳入难治组;同期104例普通肺炎支原体肺炎患儿纳入普通组。比较不同组别患儿的临床资料。测定不同组别的血清LDH和IL-6水平,以及进行胸部CT扫描,比较难治组和普通组的CT征象。使用ROC曲线评估其在难治性肺炎支原体肺炎诊断中的效能。结果:难治组患儿血清LDH和IL-6水平均高于普通组,差异均有统计学意义(P<0.05)。难治组患儿支气管壁增厚、网状影征、磨玻璃征、间质浸润组型及肺段以上实变浸润组型肺部实质的发生率均高于普通组,差异均有统计学意义(P<0.05)。ROC曲线分析表明,胸部CT征象结合LDH和IL-6水平诊断难治性支原体肺炎AUC为0.976,敏感度为94.444%,特异度为88.462%。结论:胸部CT征象结合LDH和IL-6水平检测在诊断难治性肺炎支原体肺炎中具有重要价值,可作为有效的诊断指标。

关键词： 胃癌   白细胞介素-6   胃蛋白酶原Ⅰ   胃蛋白酶原Ⅰ/Ⅱ比值   预后  

摘要： 目的 探讨胃癌患者血清白细胞介素-6(IL-6)、胃蛋白酶原水平变化对预后的评估价值。方法 选取2019年1月至10月本院收治的胃癌患者51例。手术前采集胃癌患者空腹肘静脉血4 ml,电化学发光法测定血清IL-6水平,酶联免疫吸附法测定血清胃蛋白酶原Ⅰ、胃蛋白酶原Ⅱ水平,计算胃蛋白酶原Ⅰ/Ⅱ比值。收集患者性别、年龄,合并糖尿病、高血压情况,肿瘤分化程度,TNM分期;术后进行为期3年的随访,记录患者生存情况,预后良好患者纳入预后良好组,死亡患者纳入预后不良组。结果 51例胃癌患者随访3年期间生存31例(60.78%)纳入预后良好组,死亡20例(39.22%)纳入预后不良组,患者平均生存时间为(31.60±3.38)个月。预后不良组患者血清IL-6水平明显高于预后良好组,胃蛋白酶原Ⅰ、胃蛋白酶原Ⅰ/Ⅱ比值水平明显低于预后良好组,差异均有统计学意义(均P<0.05)。受试者工作特征(ROC)曲线分析显示,血清IL-6、胃蛋白酶原Ⅰ及胃蛋白酶原Ⅰ/Ⅱ比值对胃癌患者预后具有较高的评估价值(P<0.01);其中各指标联合检测对胃癌患者预后的评估价值最高(AUC=0.951),灵敏度明显高于各指标单独检测。肿瘤分化程度、TNM分期在不同IL-6、胃蛋白酶原Ⅰ水平的胃癌患者间比较差异均有统计学意义(均P<0.01);其中IL-6>10.31 pg/ml的胃癌患者肿瘤分化程度明显低于≤10.31 pg/ml的患者,TNM分期明显高于≤10.31 pg/ml的患者;胃蛋白酶原Ⅰ≤29.79 ng/ml的胃癌患者肿瘤分化程度明显低于>29.79 ng/ml的患者,TNM分期明显高于>29.79 ng/ml的患者。结论 血清IL-6、胃蛋白酶原Ⅰ及胃蛋白酶原Ⅰ/Ⅱ比值与胃癌患者预后生存密切相关,IL-6、胃蛋白酶原Ⅰ水平与肿瘤分化程度、TNM分期存在相关性,可用于评估患者病情和预后。

关键词： Alzheimer's disease   neuroinflammation   brain-targeted drug delivery   tau neuropathology   reactive oxygen species  

摘要： Alzheimer'sdisease(AD)isaprogressive neurodegenerative disorder with prolonged latency during the prodromal ***,current clinical approaches to pharmacological interventions for AD remain *** effective treatment strategy is to eliminate protein aggregates and prevent their *** this study,interleukin(IL)-3-loaded porous manganese(Mn^(2+))-doped Prussian blue(PB)nanoparticles(NPs)were coated with dopaminergic MEs23.5 neuronal cell membranes(PBMn-IL3@CM)and then used to combat AD *** PBMn-IL3@CM NPs possessed a high targeting efficiency,being able to traverse the blood-brain barrier and specifically affect the brain to reduce oxidative *** Mn2-doped PB NPs effectively scavenged reactive oxygen radicals produced by microglia,inducing the pro-inflammatory M1 *** addition,the released IL-3 activated microglia,causing the polarization of the M1 phenotype to the anti-inflammatory M2 phenotype in vitro and in *** transition reduced phosphorylated tau accumulation,mediated neurotoxicity,and eliminated tau aggregates,which reversed cognitive impairment in AD *** PBMn-IL3@CM NPs showed excellent biocompatibility without significant adverse effects;consequently,they represent a promising AD treatment.

关键词： 免疫球蛋白   膀胱肿瘤钬激光切除术   膀胱尿路上皮癌   复发   相关性  

摘要： 目的:探讨免疫球蛋白水平与膀胱肿瘤钬激光切除术后复发的相关性。方法:选取行膀胱肿瘤钬激光切除术治疗的80例膀胱尿路上皮癌患者作为研究对象。根据术后是否复发分为复发组(n=20)及未复发组(n=60)。比较两组患者淋巴细胞亚群、免疫球蛋白及补体水平变化情况;采用Spearman法进行相关性分析;制作受试者工作特征(ROC)曲线,并以曲线下面积(AUC)分析各指标对术后复发的预测价值。结果:术前、术后3、6个月及1年时,复发组自然杀伤细胞(NK)、CD4^(+)T淋巴细胞占比、CD4^(+)/CD8^(+)比值、免疫球蛋白A(IgA)、免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、补体C3及补体C4水平均低于未复发组(P<0.05)。术前及术后1、3个月,复发组CD8^(+)T淋巴细胞占比高于未复发组(P<0.05)。术前NK、CD4^(+)T淋巴细胞占比、CD4^(+)/CD8^(+)比值、IgA、IgG、IgM、补体C3及补体C4均与术后复发负相关(P<0.05),CD8^(+)T淋巴细胞占比与术后复发正相关(P<0.05)。ROC曲线分析显示,该类指标均对患者术后复发均有较高预测价值,各指标联合预测的AUC为0.890,敏感度为0.800,特异度为0.900(P<0.05)。结论:术前NK、CD4^(+)T淋巴细胞占比、CD4^(+)/CD8^(+)比值、IgA、IgG、IgM、补体C3和补体C4水平偏低及CD8^(+)T淋巴细胞占比水平偏高均提示患者术后复发风险较高,各指标联合检测可用于临床辅助预测患者预后情况。

关键词： REDD1   Nrf2   IL-33   Airway epithelium cells   Asthma  

摘要： Objective: This study aims to investigate whether REDD1 (Regulated in Development and DNA Damage Responses 1) mediates the nuclear-to-cytoplasmic translocation and release of IL-33 in airway epithelial cells induced by house dust mites (HDM). Methods: REDD1 expression levels in bronchial asthma patients were validated using public databases, followed by immunohistochemical analysis of REDD1 protein in airway epithelial cells from these patients. An asthma model was then established using HDM-induced 16HBE cell lines, with REDD1 gene knockout performed. The relationship between varying levels of REDD1 expression, Nrf2, and related inflammatory factors was assessed using Western blot and qPCR. To further investigate the role of the REDD1-Nrf2-IL-33 axis in the development of asthma, we employed Nrf2 activators and inhibitors to reassess the impact of REDD1 on IL-33. Results: At both mRNA and protein levels, we found that REDD1 was significantly overexpressed in samples from asthma patients (P < 0.05). In vitro, 24-hour exposure to HDM induced a notable nuclear-to-cytoplasmic translocation of IL-33 and increased its levels in the culture medium of 16HBE cells. In addition, HDM treatment significantly upregulated the expression of both REDD1 and Nrf2. Knockdown of REDD1 markedly suppressed HDM-induced IL-33 release and the expression of TNF-alpha, IL-6, and IL-1 beta, while enhancing Nrf2 expression. Moreover, treatment with the Nrf2 agonist curcumin inhibited HDM-induced nuclear-to-cytoplasmic translocation and extracellular secretion of IL-33, whereas the opposite effect was observed when using the Nrf2 antagonist ML385. Conclusion: This study reveals the crucial regulatory role of the REDD1-Nrf2-IL-33 axis in the pathological process of bronchial asthma. REDD1 modulates the expression of IL-33 and other inflammatory factors through the Nrf2 signaling pathway, thereby influencing the onset and progression of asthma.

关键词： 鸭坦布苏病毒   E蛋白   原核表达   多克隆抗体   间接ELISA  

摘要： 旨在制备鸭坦布苏病毒(DTMUV)E蛋白多克隆抗体并建立抗体检测方法。克隆DTMUV E基因,构建重组质粒pET-32a-E并进行原核表达,对表达蛋白提纯后免疫家兔,制备兔抗DTMUV E蛋白多克隆抗体。以原核表达的E蛋白作为包被抗原,建立了检测抗体的间接ELISA方法。结果:DTMUV E蛋白在大肠杆菌中成功表达,且具有良好的免疫原性,制备的兔源抗DTMUV E蛋白多克隆抗体能够与DTMUV发生良好特异性反应,建立的抗体间接ELISA检测方法具有高敏感性、特异性和重复性。提示:制备的DTMUV多克隆抗体和建立的抗体间接EIISA检测方法为DTMUV的深入研究提供了技术支撑。

关键词： 藏红花素   淋巴细胞   树突状细胞  

摘要： 研究藏红花素对体外脾脏淋巴细胞增殖和树突状细胞(dendritic cell,DCs)成熟的影响,采用CCK8方法检测经藏红花素刺激后淋巴细胞的增殖情况,然后体外原代分离小鼠骨髓前体细胞,细胞因子诱导分化形成未成熟的DCs,藏红花素刺激DCs 24 h后,流式检测DCs表型标志,利用ELISA方法检测细胞因子的分泌情况。结果显示:相比对照组,藏红花素终质量浓度为100、200和400μg/mL时,淋巴细胞增殖均极显著增强;藏红花素终质量浓度为200和400μg/mL时,DCs表型标志(CD40、CD80、CD86和MHCⅡ)均极显著上调;藏红花素终质量浓度为200和400μg/mL时,DCs上清中促炎细胞因子(TNF-α和IL-6)和抗炎细胞因子IL-10的分泌量均极显著上调。结论:藏红花素显著刺激体外脾脏淋巴细胞的增殖,促进DCs成熟。

关键词： IL-38   cytokine   periodontal diseases   inflammation   salivary  

摘要： The goal of the current study was to assess levels of salivary interleukin (IL)-38, IL-1 beta, and IL-10 in various periodontal clinical conditions. In total, 60 (20 healthy, 20 gingivitis, and 20 stage II-III, grade A-B periodontitis) subjects were included in the study. Demographic and clinical periodontal parameters were recorded. Samples were examined for IL-38, IL-1 beta, and IL-10 levels by means of enzyme-linked immunosorbent assay. Results demonstrated that the periodontitis group had significantly lower salivary IL-38 levels (P < 0.05) than the healthy group. Salivary IL-10 levels did not differ significantly between the groups (P > 0.05). The salivary IL-1 beta levels of gingivitis (P < 0.001) and periodontitis groups (P < 0.01) were significantly higher than those of the healthy group. The present study indicated that IL-38 level is decreased in periodontal disease. The results suggested a possible role of IL-38 in the periodontal inflammation process. Clarifying the mechanisms of IL-38 in the inflammatory process may contribute to the development of novel treatment strategies in periodontal diseases.

关键词： 带状疱疹   血府逐瘀汤   更昔洛韦   中医证候   体液免疫  

摘要： 目的 观察血府逐瘀汤联合更昔洛韦治疗带状疱疹的疗效及对体液免疫相关指标的影响。方法 患者88例随机分为观察组与对照组各44例。对照组给予更昔洛韦治疗,观察组在对照组的基础上联合血府逐瘀汤治疗。比较两组中医证候疗效、免疫球蛋白、T细胞亚群、病情严重程度以及不良反应的发生情况。结果治疗组总有效率为93.18%,高于对照组的77.27%(P <0.05);观察组总不良反应发生率为4.55%,低于对照组的18.18%(P <0.05)。治疗2周后,两组T细胞亚群(CD3+、CD4+、CD4+/CD8+)、免疫球蛋白(IgA、IgG、IgM)水平均高于治疗前,CD8+低于治疗前(P <0.05);观察组CD3+、CD4+、CD4+/CD8+和IgA、IgG、IgM水平高于对照组(P <0.01),CD8低于对照组(P <0.01)。结论 血府逐瘀汤联合更昔洛韦治疗带状疱疹可改善患者临床症状,提高患者机体免疫水平,其疗效优于单纯更昔洛韦治疗。