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关键词： 18β-glycyrrhetinic acid   Apoptosis   Bisphenol A   Hepatotoxicity   Inflammation   Nephrotoxicity  

摘要： Bisphenol A (BPA) has been commonly used in various consumer products, including water bottles, food containers, and canned food linings. However, there are concerns about its potential toxicity to human health, particularly its impact on the liver and kidneys. The objective of this research was to investigate the potential ameliorative effects of 18β-glycyrrhetinic acid (GA) against BPA-induced hepatotoxicity and nephrotoxicity in rats. The animals were supplemented with BPA (250 mg/kg b.w.) alone or with GA (50 and 100 mg/kg b.w.) for 14 days. GA treatment alleviated the BPA-induced hepato-renal tissue injuries through reducing the serum ALT, AST and ALP levels, and urea and creatinine levels. GA co-treatment also increased activities of SOD, CAT and GPx enzymes and levels of GSH, and suppressed MDA levels in BPA induced tissues. BPA also induced inflammation by increasing the levels of TNF-α, NF-κB, JAK1, STAT1, P38 MAPK and JNK in liver and kidney tissues and GA treatment ameliorated these effects. BPA triggered apoptosis by increasing caspase-3, Bax, and cytochrome c at protein levels and also by decreasing the antiapoptotic Bcl-2 level. However, treatment with GA (50 and 100 mg/kg) decreased apoptosis. Overall, our results have revealed the potential ameliorative mechanisms of GA, as a possible agent for BPA-induced hepatotoxicity and nephrotoxicity. © 2024 Elsevier Ltd

关键词： A型塞内卡   VP2蛋白   原核表达   多克隆抗体   竞争ELISA  

摘要： 依据A型塞内卡病毒(SVA)结构蛋白VP2基因序列构建原核表达质粒pET-30a-VP2,经转化和诱导,表达出VP2重组蛋白,纯化后免疫新西兰大白兔,采集血清获得VP2的多克隆抗体。用间接ELISA方法测定血清中VP2抗体效价,并用Western blot与IFA验证其特异性。随后建立竞争ELISA方法,并对实验室收集的2016-2019年广西11个地区64个规模猪场592份猪血清进行SVA抗体检测。结果显示,诱导表达出的重组VP2蛋白主要以可溶性形式表达,血清中VP2抗体效价大于1∶64000,且制备的VP2抗体能特异结合SVA感染细胞中的VP2蛋白。竞争ELISA检测结果显示广西地区猪场SVA血清阳性率高达76.6%,血清样品阳性率高达55.9%,研究结果为SVA的检测和研究奠定了基础。

关键词： Kidney transplantation   HLA-C   HLA-DP   HLA-DQ   Donor specific antibodies   Review  

摘要： HLA-C,HLA-DP and HLA-DQ are thought to be benign due to low expression and few initial negative ***,most allocation programs used HLA-A,HLA-B and HLA-DR antigens for *** the advent and use of single-bead antigen assays,more was learned about donor-specific antibodies(DSAs)against these *** in these antigens and antibodies grew when cases of acute antibody-mediated rejection(AMR),mixed rejections,chronic AMR,and reduced graft survival were reported with DSAs against these *** the deleterious effects of these DSAs are more pronounced in retransplants,harmful effects have also been observed in first-time *** against each of these antigens can trigger rejection *** combination with DSAs against HLA-A,HLA-B and HLA-DR can cause more *** has been shown that strategies that reduce mismatches for these antigen lead to fewer rejections and better graft *** is a need for greater consensus on the universal typing of these antigens prior to transplantation for better patient and graft *** review focuses on the interaction of these antigens with lymphocytes and killer immunoglobulin receptors,arguments for not typing them,detailed analyses of the literature about their harmful effects,potential strategies moving forward,and recommendations for the future.

关键词： asthma   Chronic rhinosinusitis with nasal polyps   comorbidity   nasal polyps   real world   severe asthma   SNOT-22   T2 inflammation  

摘要： Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) and severe asthma (SA) are 2 frequently coexisting conditions that are, in most cases, associated with eosinophilic inflammation. The concurrence of both diseases has a negative synergistic impact on disease severity and patients’ health-related quality of life. Thus, a holistic, collaborative management of these patients is a critical unmet need. Mepolizumab, a systemic anti–IL-5 therapy, has been shown to be effective as an add-on treatment in both SA and CRSwNP, with more literature available on asthma outcomes than on CRSwNP. Objectives: The primary objective of the study is to evaluate the real-world effectiveness of mepolizumab in improving the health-related quality of life of comorbid patients at 12 months using the SNOT-22 questionnaire. Secondary objectives include safety and efficacy outcomes of mepolizumab treatment in the 2 populations, which are expected to have variable severity of the respective comorbid conditions. Methods: RESPONSE is a European real-world prospective cohort study designed to assess the effectiveness of mepolizumab in 2 cohorts of adult patients: one with SA as primary diagnosis with (secondary diagnosis) comorbid CRSwNP, and another with CRSwNP as primary diagnosis with (secondary diagnosis) comorbid asthma. Up to 350 patients receiving newly prescribed mepolizumab will be followed up for 12 months as per the investigators’ standard of care. Conclusion: This study will report the effects of anti–IL-5 therapy in both diseases investigated and the respective comorbidity, as well as the consequence of treating milder forms of asthma and CRSwNP with mepolizumab, supporting the emerging evidence on early treatment optimization. © 2024 The Authors

关键词： 南美白对虾   甲壳类动物   特异性免疫   凡纳滨对虾   出肉率   细胞免疫   淡水虾类   免疫系统  

摘要： 南美白对虾,学名凡纳滨对虾(Penaeus Vannamei),因具有肉质鲜嫩、出肉率高、生产周期短和经济效益高等特点,深受养殖户青睐,常年居淡水虾类养殖产量第一。南美白对虾属于无脊椎甲壳类动物,其免疫系统相对简单和脆弱,通常只依赖细胞免疫作为主要防线,缺乏特异性免疫系统。

关键词： CD36   单克隆抗体   血小板清除   Fc依赖  

摘要： 目的探讨CD36抗体在体内介导血小板吞噬清除的作用机制。方法将单克隆抗体(mAb)32-106(IgG2a)以2 mg/kg的浓度注射给C57BL/6J雌鼠,并分别在注射前0 h、注射后0.5 h、1 h、2 h、4 h、6 h及24 h检测小鼠体内血小板数量,通过胃蛋白酶消化处理,获得32-106的F(ab')_(2)段,并将其注射给C57BL/6J雌鼠,分析其清除CD36(+)血小板的效果,制备其他亚型的32-106,通过体内实验分析其对CD36(+)血小板的清除作用。结果体内实验证实,mAb 32-106 IgG2a可导致C57BL/6J雌鼠体内血小板数的明显降低(P<0.05),而mAb 32-106的F(ab')_(2)段并不引起雌鼠体内血小板的减少。C57BL/6J雌鼠接受2 mg/kg的32-106 IgG1或32-106 IgG3,也发生血小板数量降低,但与32-106 IgG2a相比,IgG1及IgG3亚型32-106引起的小鼠血小板减少不明显。结论通过体内实验证实,CD36抗体以Fc依赖途径参与体内CD36(+)血小板的清除,且IgG2a亚型的抗体引起的血小板清除效果显著。

关键词： 中性粒细胞与淋巴细胞比值   糖尿病   糖尿病周围神经病变   诊断价值  

摘要： 炎症标志物中的中性粒细胞/淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)作为一个新型有效指标,因经济、易操作、敏感性等优点,为多种疾病的诊治提供指导。糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)是糖尿病主要并发症之一,为患者带来巨大痛苦,也为家庭和社会带来沉重的经济负担,故DPN的早期预防与诊治尤为重要。NLR与DPN有一定的相关性,因此,本文介绍了NLR在DPN中的研究进展,经检索发现NLR可预测糖尿病患者未来发生DPN的风险,并对其诊断具有一定意义。高水平的NLR与DPN的严重程度呈正相关,且可对DPN的后续治疗有指导意义。

关键词： 脑膜癌   MRI   清蛋白   免疫球蛋白   诊断  

摘要： 目的 分析血清清蛋白与免疫球蛋白联合MRI对脑膜癌的诊断价值。方法 选取疑似脑膜癌患者100例,经脑脊液细胞学检查确诊为脑膜癌66例(脑膜癌组),非脑膜癌34例(非脑膜癌组)(其中结核性脑膜炎20例,原发性颅内肿瘤13例,其他1例)。比较两组血清清蛋白与免疫球蛋白水平,ROC曲线分析MRI联合血清清蛋白、免疫球蛋白对脑膜癌的诊断价值。结果 脑膜癌组ALB水平低于非脑膜癌组(P<0.05),IgG、IgM高于非脑膜癌组(P<0.05),两组IgA比较,P>0.05;ALB最佳阈值为36.125 g/L,灵敏度为0.735,特异度为0.788;IgG最佳阈值为8.765 g/L,灵敏度为0.803,特异度为0.676;IgM最佳阈值为1.58 g/L,灵敏度为0.652,特异度为0.676;MRI联合血清清蛋白与免疫球蛋白对脑膜癌诊断灵敏度为93.94%(62/66)、阴性预测值为87.50%(28/32),均高于单一诊断指标(P<0.05)。结论 MRI联合血清清蛋白与免疫球蛋白可以显著提高脑膜癌病的诊断灵敏度和阴性预测值,具有很高的临床应用价值。

关键词： 猫传染性腹膜炎病毒   N蛋白   单克隆抗体   竞争ELISA  

摘要： 为建立抗猫传染性腹膜炎病毒(feline infectious peritonitis virus,FIPV)抗体竞争ELISA检测方法,应用原核表达FIPV截短N蛋白[rFIPV N(aa17-172)]免疫BALB/c小鼠,经细胞融合和间接ELISA方法筛选能稳定分泌针对重组N蛋白(17-172 aa)的杂交瘤细胞株,制备腹水并进行纯化和鉴定。以重组N蛋白为抗原,纯化后的单克隆抗体为一抗建立抗体竞争ELISA方法。试验结果显示,纯化后重组N蛋白浓度为0.5 mg/mL,纯度大于85%,将其作为包被抗原,通过间接ELISA筛选出3株单克隆杂交瘤细胞株,命名为5C5、1F8和4A10。纯化的3株单克隆抗体效价均高于1∶102400。间接免疫荧光试验结果显示,3株单克隆抗体均能与FIPV发生特异性反应。抗体竞争ELISA方法最佳包被抗原浓度为1.25μg/mL,最佳抗体工作浓度为1∶128000。通过检测本实验室保存的94份FIPV阴性血清,确定本研究建立的抗体竞争ELISA方法阳性阻断率≥20.1%,阴性阻断率≤15.9%。对62份猫血清和本实验室前期建立的FIPV抗S蛋白的抗体竞争ELISA方法进行符合率比较,两种方法符合率为85.5%。本研究建立的抗N蛋白抗体竞争ELISA方法可用于FIPV抗体的检测,为FIP诊断提供了技术支撑。

关键词： 非洲猪瘟病毒   pB602L蛋白   多克隆抗体   病毒活载体  

摘要： 为深入研究非洲猪瘟病毒(African swine fever virus,ASFV)的编码基因B602L,本研究根据ASFVSY18株的B602L基因序列(MH766894.3),设计引物合成1593 bp的B602L基因全长序列,构建pCold-TFB602L原核表达质粒。将鉴定正确的原核表达质粒转化感受态细胞,用IPTG诱导表达重组蛋白B602L并通过SDS-PAGE进行验证和分析。用纯化后的pB602L蛋白免疫家兔制备多克隆抗体,测定抗体效价并经Westernblot鉴定其特异性。同时基于本实验室前期研究,构建表达B602L基因编码蛋白的重组猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)全长感染性克隆。本研究将ASFV B602L基因全长序列插入PRRSVORF1b和ORF2a之间,转染全长感染性克隆质粒后拯救获得重组病毒rPRRSV-B602L,并对其进行病毒学特性分析。利用制备的多克隆抗体与重组病毒和真核细胞表达的蛋白进行Western-blot和IFA验证。结果显示,成功构建pCold-TF-B602L原核表达质粒,诱导表达的重组蛋白pB602L可与His标签单抗和制备的抗pB602L抗体产生特异性反应,其效价为1∶16000;成功构建并拯救出重组病毒rPRRSV-B602L,它与亲本病毒具有相似的病毒学特性。本研究拯救的重组病毒可以稳定表达pB602L蛋白,该抗pB602L多克隆抗体能够特异性识别重组病毒表达的蛋白和真核细胞表达的蛋白,具有良好的特异性和反应原性,为进一步探索pB602L蛋白的功能及研制表达ASFV特异性抗原蛋白的重组PRRSV活载体疫苗提供一定的研究基础。