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关键词： HLA-B gene   HLA-B*58:01   rs9263726 SNP   gout patients   paraclinical characteristics  

摘要： Background/Objectives: The HLA-B*58:01 allele is strongly linked to severe cutaneous adverse reactions (SCARs) during allopurinol treatment, and it has been associated with the A allele of PSORS1C1 rs9263726 (G>A). Paraclinical characteristics of gout are indicative of associated comorbid conditions. This study investigated the genotype frequency of HLA-B*58:01 and its association with paraclinical characteristics and PSORS1C1 rs9263726 in gout patients from Northeast Vietnam. Methods: A total of 133 unrelated gout patients were randomly recruited by the clinician. BioEdit sequence alignment editor version 7.2.5 software (Raleigh, Raleigh, NC, USA) was used for the analysis of nucleotide sequence data of HLA-B gene alleles from the IPD-IMGT/HLA Database, which showed that the HLA-B*58:01 allele can be distinguished from reference and other alleles by specific nucleotide positions: 387C, 379C, 368A, 355A, and 353T (in exon 3);and 319C, 285G, and 209A (in exon 2). HLA-B*58:01 and PSORS1C1 rs9263726 genotypes were identified using Sanger sequencing of PCR products, analyzed with BioEdit software, and verified using the NCBI dbVar database. Statistical analyses were performed using SPSS version 25.0. Results: Our study revealed a significant age difference between male and female gout patients (p < 0.001). Male gout patients had an average age of 51.44 +/- 14.59 years, whereas female gout patients were notably older, with an average age of 70.33 +/- 10.64 years. Positive correlations were observed between platelet count, serum creatinine, and uric acid levels (r = 0.174, p = 0.045;r = 0.195, p = 0.025) in male gout patients, while only high-density lipoprotein cholesterol showed a statistically significant negative correlation with uric acid levels (r = -0.885, p = 0.002) in female patients. The HLA-B*58:01 allele frequency among study subjects was 6.02%, with 12.03% being heterozygous individuals (*X/HLA-B*58:01, N = 16). The HLA-B*58:01 allele was not detected in fema

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关键词： 骨关节炎   香菇多糖   软骨细胞   细胞外基质   ROS/NF-κB信号通路  

摘要： 目的：探讨香菇多糖（LNT）对白细胞介素-1β(IL-1β)诱导的软骨细胞的作用及机制。方法：采用CCK-8法检测不同浓度LNT(0、50、100、200、400和800μg·mL-1)处理24 h或72 h后对人软骨细胞活性的影响。随后将软骨细胞分为对照组、IL-1β组(10 ng·mL-1)、LNT(100μg·mL-1)+IL-1β组和LNT(200μg·mL-1)+IL-1β组，EdU染色检测LNT对IL-1β处理后软骨细胞增殖水平；Western Blot检测软骨细胞基质金属蛋白酶13（MMP13）、血小板反应蛋白解整合素金属肽酶5（ADAMTS5）、Ⅱ型胶原蛋白α1链（Col2a1）和p-P65的蛋白表达；免疫荧光染色检测软骨细胞MMP13、Col2a1的蛋白表达及p-P65核转位；荧光探针DCFH-DA检测软骨细胞活性氧（ROS）的产生。结果：低浓度LNT(50、100、200和400μg·mL-1)较对照组活性无显著性差异，而与LNT(400μg·mL-1)相比，LNT(800μg·mL-1)显著抑制软骨细胞活性（P<0.05）；此外，EdU染色显示LNT(200μg·mL-1)显著改善IL-1β诱导的增殖抑制（P<0.05）；另外，LNT能够显著抑制IL-1β诱导的软骨细胞基质降解酶MMP13和ADAMTS5的蛋白表达，并促进软骨基质Col2a1的蛋白表达（P<0.05或P<0.01）；机制层面，IL-1β诱导的软骨细胞ROS的产生及p-P65核转位能被LNT显著抑制（P<0.05或P<0.01）。结论：LNT通过抑制ROS/NF-κB信号通路，改善IL-1β诱导的软骨细胞增殖抑制及基质降解，为探索骨关节炎新的药物治疗策略提供实验依据。

关键词： CD147   IL-6   primary liver cancer   invasion and metastasis   nomographic prediction model  

摘要： Objective: This study aims to develop a prediction model for invasive metastasis of primary liver cancer based on serum extracellular matrix metalloproteinase-inducing factor (CD147) and interleukin-6 (IL-6). Methods: Between July 2022 and August 2024, 170 surgically treated primary hepatocellular carcinoma patients at our hospital were recruited. They were divided into a training group (n = 120) and a validation group (n = 50) at a 7:3 ratio. Univariate and multivariate logistic regression analyses were applied in the training group to identify factors related to invasive metastasis. A risk factor-based bar chart prediction model was then constructed and internally tested. Its goodness of fit was evaluated, and the model's diagnostic efficacy was assessed using the ROC curve. Finally, decision curve analysis (DCA) was performed to evaluate the model's clinical value. Results: In the training group, compared with the noninvasive metastasis group, patients in the invasive metastasis group had a significantly lower percentage of intact envelope and tumor size >= 5 cm, and significantly higher serum alpha-fetoprotein (AFP), alkaline phosphatase (ALP), C-reactive protein/albumin ratio (CAR), oncoglobulin (CEA), CD147, and IL-6 levels (all p < 0.05). After logistic multifactorial analysis, intact envelope, tumor > 5 cm, AFP, CAR, CD147, and IL-6 were identified as independent influencing factors for invasive metastasis of primary hepatocellular carcinoma (all p < 0.05). A column chart model was constructed. The C-index of the training and validation groups was 0.884 (95% confidence interval [CI]: 0.738-0.932) and 0.841 (95% CI: 0.741-0.939), respectively. The calibration curves showed good agreement between the predicted probability and the actual probability in both the training and validation groups, without significant deviation. The area under the curve (AUC) of the ROC analysis was 0.852 (95% CI: 0.824-0.979) and 0.839 (95% CI: 0.791-0.912), respectively. DCA indica

关键词： Alzheimer's disease (AD   IL-1 family of cytokines   neuroinflammation  

摘要： Alzheimer's disease (AD) is a long-term, progressive, degenerative disorder. One of the most important pathological characteristics of AD is the deposition of beta-amyliod (A beta) peptide, which initiates a spectrum of cerebral neuroinflammation. Vascular changes also play an important role in the pathophysiology of the disease. Cytokines, secreted by immune cells, can facilitate cell-to-cell signaling and influence the functions of the central nervous system (CNS). These important mediators of the immune system, which are known to orchestrate various molecular and cellular mechanisms in both physiological and pathological situations, can be upregulated or downregulated, leading to a complex crosstalk with numerous receptors mediating pro-inflammatory and/or anti-inflammatory actions. In particular, the interleukin (IL)-1 family of cytokines has been implicated to significantly correlate with AD pathogenesis among other cytokines in the CNS. The IL-1 family of cytokines is essential in both the innate and adaptive immune responses. This pleiotropic family of cytokines includes IL-1 alpha, IL-1 beta, IL-1 receptor antagonist (RA), IL-18, IL-33, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-36RA, IL-37, and IL-38. Recent studies have demonstrated that the upregulation of pro-inflammatory cytokines, such as IL-1 alpha and IL-1 beta, or the downregulation of anti-inflammatory mediators, exerts multifaceted influences on both neurodegeneration and neuroprotection. The lack of effective treatment for AD necessitates the search for new drugs that target several processes in the disease's pathology. This review aims to give a comprehensive overview of the emerging roles of the IL-1 family of cytokines in AD pathology and to explain their perspectives on introducing novel strategies for effective therapeutic/neuropsychiatric management of AD in clinical settings by discussing both the pathogenic and protective roles of these cytokines.

关键词： 新生儿败血症   IL-6   IL-8联合PCT   CRP   诊断  

摘要： 探讨白细胞介素-6(IL-6)、白细胞介素-8(IL-8)联合降钙素原(PCT)、C反应蛋白(CRP)在新生儿败血症早期诊断中的效果,以提高新生儿败血症的诊断敏感性和精确度,为对新生儿败血症的早期发现提供更多依据。方法 收治的100例疑似新生儿败血症患儿于2023年1月至2024年3月内进行收治,按不同的诊断方式把患儿随机分为对照组(50例)、试验组(50例)。对照组以常规血培养与单一检测生物标志物诊断,试验组以联合检测IL-6、IL-8、PCT、CRP进行早期诊断。通过比较两组的诊断效能、诊断时间差异以及后续预后情况,评估联合检测方案的诊断优势。结果 实验组在诊断灵敏度、特异性和准确度上显著高于对照组。联合检测不仅能够缩短诊断时间,减少延误治疗的风险,还能对新生儿败血症的早期识别起到积极作用。此外,实验组患儿的临床预后明显优于对照组,死亡率和并发症发生率明显降低。结论 IL-6、IL-8联合PCT、CRP的检测方案在新生儿败血症的早期诊断中展现了较高的临床价值。这一方案能够有效缩短诊断时间,提高治疗及时性,并对改善患儿的预后产生积极影响。因此,联合检测不仅具有实际应用前景,也为新生儿败血症的早期诊断提供了新的思路和方法。

关键词： 免疫球蛋白   美罗培南   新生儿败血症   炎症反应   免疫水平  

摘要： 分析免疫球蛋白与美罗培南治疗对新生儿败血症患儿的效果。方法 选取我院的60例败血症新生儿患儿,分入研究组(30例,采用免疫球蛋白联合美罗培南治疗)和对照组(30例,采用美罗培南治疗),比较两组效果。结果 研究组效果优于对照组(P<0.05)。结论 免疫球蛋白联合美罗培南治疗对败血症患儿的效果优于单独美罗培南治疗,更有利于促进患儿炎症反应与免疫指标改善,提升总体疗效,缩短症状消除时间。

关键词： 儿童川崎病   静注人免疫球蛋白   阿司匹林   氯吡格雷   效果评估  

摘要： 进一步剖析川崎病儿童分别应用阿司匹林联合静注人免疫球蛋白及氯吡格雷联合静注人免疫球蛋白的治疗效果与价值,为不适合应用阿司匹林药物治疗的川崎病儿童在药物选择上提供可靠性分析。方法 一共纳入100例川崎病患儿,全部患儿均来源上林县人民医院,使用黑白球法分为例数均衡的两组,形成对照组(n=50)和观察组(n=50),对应采用静注人免疫球蛋白尿联合阿司匹林和静注人免疫球蛋白联合氯吡格雷,并量化评估最终的治疗效果和应用价值。结果 两组对比,在炎症水平、BNP水平、临床总有效率、不良反应以及住院时间等方面均无统计学意义(P>0.05)。结论 在儿童川崎病的治疗中,静注人免疫球蛋白联合氯吡格雷治疗方案在降低炎症指标无明显优势,在总体疗效、药物不良反应与传统应用静注人免疫球蛋白联合阿司匹林无明显差异,应用于合并阿司匹林禁忌症的川崎病患儿是有效的、安全的。

关键词： 急性淋巴细胞白血病   诱导缓解   化疗   护理干预   疗效评估  

摘要： 钻研急性淋巴细胞白血病(ALL)患者诱导缓解化疗期间护理干预的作用,估量护理手段对患者化疗成果、不良反应、生活水平及疗效延续时间的效应,方法 这项研究选取80例急性淋巴细胞白血病患者入组,随机把对象分为对照组与观察组,分别采用常规化疗及常规化疗结合个性化护理方式。对比两组化疗后缓解比率、不良反应出现率、生活质量得分以及疗效持续时间,结果 观察组完全缓解率为45.00%这一数值,显著比对照组的25.00%要高;观察组呈现出82.50%的总体缓解率,显著高于对照组的65.00%,p<0.001,差异极为显著;观察组出现不良反应的比率明显低于对照组,尤其是恶心呕吐出现的概率,p计作0.014;观察组体现出的无病生存时长为9.2个月,显著胜过对照组的6.5个月,结论 护理干预可大幅提升急性淋巴细胞白血病患者的治疗成效,降低不良反应几率,提高生活质量水平,并可延长治疗效果的维持期,暗示护理干预应成为ALL治疗的关键成分。

关键词： 5-hydroxymethylcytosine   cytokine   inflammation   mesenchymal stem cells   obesity  

摘要： BACKGROUND:Mesenchymal stromal cells (MSCs) possess therapeutic properties that mediate repair. Obesity impairs MSC functionality and therapeutic efficacy, possibly by eliciting dynamic modifications of epigenetic markers, like 5-hydroxymethylcytosine (5hmC). We hypothesized that human obesity alters the 5hmC landscape and anti-inflammatory capacity of adipose tissue-derived MSCs to activate the prominent inflammatory signaling mediator Interleukin (IL)-1β. METHODS:Adipose tissue samples were collected from obese and lean individuals (body mass index ≥30 or < 30kg/m2, respectively, n = 11 each) during weight-loss or kidney donation surgery. MSCs were harvested and analyzed for 5hmC profiles (MeDIP-seq) and mRNA expression (RNA-seq) (n = 5 each). Subsequently, MSCs or a vehicle were injected into mice, (n = 6 each) and two-weeks later, kidneys were evaluated using in-vivo magnetic resonance imaging and ex-vivo studies. The role of interleukin(IL)-1β was then studied in-vitro in MSC-induced immunomodulation using siRNA in macrophages. RESULTS:Compared to MSC from lean patients, obese-MSC genes showed 2,087 differential 5hmC modifications and 175 differential mRNA expression. Among them, 14 genes with overlapping alterations were involved in regulation of cytokine production, prominently IL-1β. Injecting obese MSCs elevated renal expression of IL-1β and M1 macrophage count but lowered kidney perfusion. Silencing IL-1β in obese-MSCs in vitro reduced M1 phenotype switching in co-incubated macrophages. CONCLUSION:Obesity induces epigenetic and gene expression changes in MSCs, particularly in IL-1β, associated with impaired anti-inflammatory functionality of MSCs. Targeting IL-1β could be a useful therapeutic approach to modulate the decline in MSC functionality resulting from obesity.