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关键词： IL-37   inflammation   oxidative stress   sepsis-induced lung injury   TGF-beta/Smad3  

摘要： Introduction: Sepsis is caused by an uncontrolled inflammatory response and immune dysfunction, with lung injury being the most common complication and one of the leading causes of death in clinically ill patients. Interleukin 37 (IL-37) is a multifunctional cytokine that plays a vital role in various pathophysiological processes, including inflammation, infection, and ***: The study involved both clinical and animal experiments (establishing an animal model of sepsis-induced lung injury). Firstly, 50 patients with sepsis-induced lung injury and 50 healthy controls were included. In addition, a more in-depth study was conducted using animal ***: IL-37, IL-6, PCT, and CRP levels were significantly higher in the sepsis-induced lung injury group. Correlation analysis revealed that IL-37 significantly correlated with IL-6, PCT, and CRP levels. In animal experiments, IL-37 significantly attenuated CLP-induced pulmonary edema and cellular injury while reducing the levels of inflammatory factors IL-6 and TNF-alpha, as well as sepsis-related inflammatory markers PCT and CRP. Moreover, IL-37 significantly downregulated the expression levels of genes and proteins of apoptosis-related molecules Caspase-3 and Bax and pathway molecules TGF-beta and Smad3. Discussion: The TGF-beta/Smad3 pathway is involved in the process of IL-37 inhibiting inflammatory response and ameliorating sepsis-induced lung injury.

关键词： modified Kugel repair   inguinal hernia   IL-6   CRP   MMP-9  

摘要： Objective To evaluate the effect of serum IL-6, C-reactive protein (CRP), and matrix metalloproteinase-9 (MMP-9) levels on the efficacy of modified preperitoneal Kugel repair in patients with inguinal *** Clinical records of 42 patients with inguinal hernias who underwent modified preperitoneal Kugel repair were retrospectively analyzed. Serum IL-6, CRP, and MMP-9 were detected before surgery and after surgery. The patients were divided into the corresponding high-expression group and low-expression group. The basic data and clinical characteristics of patients were analyzed and compared, as well as postoperative *** In patients with inguinal hernia, serum IL-6, CRP, and MMP-9 increase first and then decrease after surgery, reaching the peak value around 24 or 48 h after surgery. Patients with high-expression of IL-6, CRP, and MMP-9 had longer hospital stays and time to return to normal activities, and were more likely to have chronic abdominal pain. In addition, high-expression of IL-6 and CRP had a higher probability of postoperative VAS values and wound infection, and high-expression of IL-6 and MMP-9 were also more likely to have wound healing *** Serum IL-6, CRP, and MMP-9 levels in patients with inguinal hernia can affect the efficacy of modified preperitoneal Kugel repair and the prognosis of patients.

关键词： Signaling  

摘要： Tendon injuries heal by scar, leading to poor function. To date, the role of immune cells remains underexplored. Using a neonatal mouse model of functional tendon healing compared to adult scar-mediated healing, we identified a regenerative immune profile that is associated with type 1 inflammation followed by rapid polarization to type 2, driven by macrophages and regulatory T cells (Treg cells). Single-cell and bulk RNA sequencing also revealed neonatal Treg cells with an immunomodulatory signature distinct from adult. Neonatal Treg cell ablation resulted in a dysregulated immune response, failed tenocyte recruitment, and impaired regeneration. Adoptive transfer further confirmed the unique capacity of neonatal Treg cells to rescue functional regeneration. We showed that neonatal Treg cells mitigate interleukin-33 (IL-33) to enable tenocyte recruitment and structural restoration, and that adult IL-33 deletion improves functional healing. Collectively, these findings demonstrate that Treg cells and IL-33 immune dysfunction are critical components of failed tendon healing and identify potential targets to drive tendon regeneration.

关键词： 长效注射剂   儿童   化学偶联   融合蛋白   单克隆抗体   微球   纳米晶   水凝胶  

摘要： 近年来，我国儿童常见病发病率逐渐上升且低龄化日益显著。作为特殊人群，儿童用药与成人相比，除具有同适应证用药不同、剂量不同、辅料不同等特点以外，用药依从性差是影响其临床疗效的重要因素。与普通注射剂相比，儿童用长效注射剂可降低药物突释带来的副作用，并可大幅度减少注射次数，从而显著提高儿童患者依从性，提高临床疗效。长效注射剂应用于儿科具有显著优势。该文以药物和材料相互作用分类，综述儿童用长效注射剂研究进展，可为儿童用长效注射剂开发提供参考。

关键词： Chronic obstructive pulmonary disease   Inflammatory biomarkers   TNF-alpha   Myeloperoxidase   Pulmonary function   Prognostic value  

摘要： PurposeChronic obstructive pulmonary disease (COPD) is a progressive inflammatory disorder that requires effective biomarkers for assessing disease activity and severity. This study aimed to compare clinical characteristics, inflammatory biomarker levels, and pulmonary function between stable COPD (S-COPD) and treated COPD (T-COPD) patients, with a focus on the prognostic value of inflammatory markers such as TNF-alpha, MPO, and *** total of 81 patients were enrolled in the study, including 39 with stable COPD (S-COPD) and 42 with treated COPD (T-COPD). Clinical characteristics, lung function (measured by FEV1%), and inflammatory biomarkers (IL-6, MMP-9, SAA, MPO, TNF-alpha, and others) were assessed. Inflammatory biomarkers were compared between the two groups, and their associations with pulmonary function were examined using correlation and regression analyses. Prognostic value was assessed using ROC curve *** T-COPD group exhibited significantly more severe disease, with higher rates of exacerbations, worse quality of life (CAT and mMRC scores), and reduced lung function (FEV1%, 6-minute walk distance). Inflammatory biomarker analysis revealed no significant differences for IL-6, MMP-9, SAA, RDW, LCN2, PLR, and NLR, but TNF-alpha and MPO were significantly higher in T-COPD patients (P = 0.015 and P = 0.012, respectively). Among these biomarkers, MPO and TNF-alpha showed strong negative correlations with FEV1% in T-COPD patients (r = -0.521 and r = -0.459, respectively). ROC curve analysis indicated that TNF-alpha (AUC = 0.821) was the most predictive biomarker, followed by MPO (AUC = 0.785) and IL-6 (AUC = 0.711). Combining TNF-alpha and MPO provided the best prognostic performance (AUC = 0.878).ConclusionTNF-alpha, MPO, and IL-6 are significant biomarkers associated with disease severity and pulmonary function in T-COPD patients. The combination of TNF-alpha and MPO offers superior prognostic value, suggesting that these biomarkers may

关键词： Aquaporin-4   Brain oedema   Interleukin-1 alpha   Neutrophil   Tumour necrosis factor  

摘要： During the acute phase (1-3 days) of cerebral infarction, neutrophils and macrophages accumulate at the infarction site, inducing inflammation and cerebral oedema. However, the role of neutrophils in oedema formation after ischaemic stroke remains unclear. This study examined neutrophil involvement in cerebral oedema using a transient middle cerebral artery occlusion (tMCAO) rat model, primary cultured neutrophils, and astrocytes. Brain specimens were stained with myeloperoxidase (MPO) and lymphocyte antigen 6 complexes, locus G (Ly6G), and the number of MPO+/Ly6G+ cells was counted. Neutrophil infiltration began in the leptomeninges at 3 h, reaching the ischaemic cortex by 6 h and the striatum by 24 h, peaking at 24-48 h before declining. Neutrophils attached to endothelial walls and infiltrated the brain parenchyma, correlating with oedema severity. Infiltrating neutrophils strongly expressed IL-1 alpha and TNF in the ischaemic brain. Co-culturing LPS-activated neutrophils with astrocytes increased Aqp4 mRNA and protein expression, which was inhibited by IL-1RI and TNF antagonists. These findings suggest that activated neutrophils exacerbate cerebral oedema by inducing astrocytic AQP4 expression via IL-1 alpha and TNF in peri-infarct and ischaemic core tissues.

关键词： 猪流行性腹泻病毒   S蛋白   单克隆抗体  

摘要： 为了进一步探究猪流行性腹泻病毒（PEDV）感染的机制，利用CHO真核表达系统表达并纯化了PEDV S1蛋白，然后将该重组蛋白免疫BALB/c小鼠，经细胞融合后筛选获得了1株抗PEDV S1蛋白的单克隆抗体。通过间接免疫荧光和Western-blot等试验证明所制备的单克隆抗体可特异性地识别PEDV S蛋白。综上，成功制备了PEDV S1蛋白单克隆抗体，为PEDV的检测和后续研究奠定了基础。