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关键词： Tumor angiogenesis   ST2   IL-33   Endothelial cells   Gastric cancer  

摘要： Suppression of Tumorigenicity 2 (ST2) is a member of the interleukin-1 receptor/ Toll-like receptor superfamily, and its specific ligand is Interleukin-33 (IL-33). IL-33/ ST2 signaling has been implicated in numerous inflammatory and allergic diseases, as well as in promoting malignant behavior of tumor cells and angiogenesis. However, the precise role of ST2 in gastric cancer angiogenesis remains incompletely elucidated. We observed a significant correlation between high expression of ST2 in gastric cancer tissues and poor prognosis, along with various clinicopathological features. In vitro experiments demonstrated that the IL-33/ ST2 axis activates the PI3K/AKT/NF-kappa B signaling pathway through TRAF6, thereby promoting VEGFA-mediated tumor angiogenesis;meanwhile sST2 acts as a decoy receptor to regulate the IL-33/ST2L axis. Consistent findings were also observed in subcutaneous xenograft tumor models in nude mice. Furthermore, we investigated the molecular mechanism by which IL-33 promotes ST2L expression in GC cells via upregulation of transcription factors YY1 and GATA2 through intracellular signaling pathways.

摘要： Research into HLA-B*15:01 association with asymptomatic SARS-CoV-2 infection has so far yielded contradicting results. Using the UK Biobank cohort, we found a significant association between HLA-B*15:01 and asymptomatic infection. Our study adds more evidence for the complex role HLA alleles play in SARS-Cov-2 infection severity.

关键词： 脑出血   能量平衡相关蛋白   白细胞介素   中性粒细胞与淋巴细胞比值  

摘要： 目的探究外周血Adropin蛋白、白细胞介素6(IL⁃6)、中性粒细胞与淋巴细胞比值(NLR)对急性脑出血患者预后的预测价值。方法分析82例南通市海门区人民医院2018年9月至2023年9月期间救治的急性脑出血患者资料,治疗后进行3个月随访,采用改良Rankin量表评估预后并将患者分为预后良好组(n=49)和预后不良组(n=33)。比较两组入院时临床检查、影像学及实验室指标等基线资料,分析急性脑出血患者近期预后的影响因素,绘制受试者工作特征(ROC)曲线评价外周血Adropin、IL⁃6、NLR水平对脑出血患者预后的预测价值。结果相比预后良好组,预后不良组患者合并高血压占比、合并糖尿病占比、饮酒者占比、出血破入脑室者占比、年龄、入院时NIHSS评分、出血量以及外周血IL⁃6、NLR水平更高,差异均具有统计学意义(χ ^(2)=5.722、9.175、4.192、4.058,t=3.456、6.907、3.377、6.760、6.165,P<0.05);预后不良组患者外周血Adropin水平则低于预后良好组(t=6.430,P<0.05);多因素Logistic回归分析结果显示急性脑出血患者年龄、入院时NIHSS评分、外周血Adropin水平降低、IL⁃6及NLR水平升高是其预后不良的独立危险因素(P<0.05);ROC曲线结果显示外周血Adropin、IL⁃6、NLR联合预测患者预后不良的曲线下面积为0.943,优于单一检测(P<0.05)。结论外周血Adropin、IL⁃6、NLR对急性脑出血患者预后有一定预测价值,三者联合检测预测效能更高。

关键词： 支原体肺炎恢复期   免疫球蛋白   阿奇霉素   免疫五项   血常规  

摘要： 研究口服免疫球蛋白联合阿奇霉素对支原体肺炎(MPP)恢复期的患儿体液免疫水平及炎症水平变化的影响。方法 选取自2023年10月至2024年6月期间在本院儿科就诊确诊为MPP并经过规范治疗进入恢复期的86名患儿,随机把所有患儿分成对照组和治疗组各43例,对照组采用常规遵医嘱口服阿奇霉素序贯治疗,治疗组除阿奇霉素序贯治疗外加服免疫球蛋白,均持续治疗14d。比较两组患儿体液免疫及炎症水平的变化。结果 治疗前,免疫五项检验中,补体C3、免疫球蛋白IgG、免疫球蛋白IgA两组无明显差异,补体C4、免疫球蛋白IgM对照组高于治疗组,治疗后,治疗组免疫五项指标平均上升,观察组则均下降,且补体C4、IgM、IgA三项指标治疗组明显高于对照组(P<0.05);治疗前,炎症水平(血常规检验)中,中性粒细胞比率、血红蛋白、CRP两组无明显差异,白细胞数量治疗组高于对照组,治疗后,白细胞、中性粒细胞比率、CRP水平两组均降低,白细胞和CRP治疗组明显低于对照组(P<0.05),血红蛋白无明显差异性。结论 阿奇霉素联合免疫球蛋白临床疗效较好,可以改善MPP恢复期患儿体液免疫及炎症水平。

关键词： 镍纹样蛋白   系统性红斑狼疮   巨噬细胞极化   疾病活动度   生物标志物  

摘要： 目的探讨镍纹样蛋白(Metrnl)在系统性红斑狼疮(SLE)及其严重并发症狼疮性肾炎(LN)患者中的水平,并探讨其与巨噬细胞极化状态及疾病活动度的关联。方法选取2023年6月至2024年5月该院收治的80例SLE患者作为研究对象,分为LN组(40例)和无LN组(40例),另选取同期该院30例年龄、性别匹配的健康体检者作为对照组。采用酶联免疫吸附试验检测Metrnl、M1型标志蛋白(iNOS)、M2型标志蛋白(Arg-1)、肿瘤坏死因子(TNF)-α和白细胞介素(IL)-10水平。采用Pearson相关分析SLE患者Metrnl水平与各项常规实验室指标水平,以及iNOS、Arg-1、TNF-α、IL-10的相关性。采用受试者工作特征(ROC)曲线分析Metrnl在SLE和LN中的诊断价值。结果LN组平均Metrnl水平为(98.35±37.12)pg/mL,明显低于无LN组的(167.89±25.74)pg/mL和对照组的(257.89±22.45)pg/mL,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,SLE患者Metrnl水平与TNF-α水平均呈负相关(r=-0.328,P=0.015),与Arg-1、IL-10水平均呈正相关(r=0.538、0.489,P<0.05)。ROC曲线分析结果显示,Metrnl诊断SLE患者和健康体检者的曲线下面积(AUC)为0.88,灵敏度为90.32%,特异度为76.00%;诊断无LN患者和LN患者的AUC为0.83,灵敏度为87.10%,特异度为73.53%。结论Metrnl在SLE及LN的免疫调控中发挥重要作用,可能通过影响巨噬细胞极化状态调节疾病活动度。SLE患者血清Metrnl水平与炎症因子等临床指标水平均有明显相关性,为其作为评估疾病活动度和治疗反应的潜在生物标志物提供了理论依据。

摘要： Objective: This study aimed to investigate the changes in angiopoietin-2 and tumor necrosis factor α levels in patients with acute myocardial infarction complicated with pulmonary infection. Methods: Retrospective selection was conducted on 61 patients with acute myocardial infarction complicated with pulmonary infection and 122 patients with simple acute myocardial infarction. A comparison was made between the two groups regarding general information and serum myocs. The distribution and drug resistance of pathogenic bacteria were also explored. Results: The study showed significant differences in the duration of alcohol consumption, the proportion of diabetes mellitus, and levels of certain markers (serum cardiac troponin T, creatine kinase isoenzyme, myoglobin, angiopoietin-2, tumor necrosis factor α) between the two groups (P < .05). Logistic regression analysis identified elevated levels of serum angiopoietin-2 and tumor necrosis factor α, along with diabetes mellitus, as independent risk factors for acute myocardial infarction complicated with pulmonary infection (P < .05). Pearson correlation analysis demonstrated a positive correlation between serum angiopoietin-2 and tumor necrosis factor α levels and CPI scores in patients (P < .05). ROC curve analysis indicated that combined diagnosis of serum angiopoietin-2 and tumor necrosis factor α had an AUC of 0.867, with a sensitivity of 85.25% and specificity of 77.87% for detecting acute myocardial infarction complicated with pulmonary infection. Among the sputum culture specimens, gram-negative bacteria accounted for 55.34%, gram-positive bacteria for 39.81%, and fungi for 4.85%. Gram-negative bacteria like Klebsiella pneumoniae and Escherichia coli showed high resistance to various antibiotics, while gram-positive bacteria like Streptococcus pneumoniae and Staphylococcus aureus had relatively low resistance to specific antibiotics. Conclusion: Gram-negative bacteria were the main pathogens and exhibited resista

关键词： 变应性鼻炎   穴位注射   p38丝裂原活化蛋白激酶   炎性反应   细胞因子   黏蛋白5亚型AC  

摘要： 目的:观察穴位注射对变应性鼻炎大鼠鼻黏膜p38丝裂原活化蛋白激酶(MAPK)、磷酸化(p)-p38 MAPK、黏蛋白5亚型AC(MUC5AC)及血清相关炎性因子的影响,探讨穴位注射改善变应性鼻炎大鼠鼻黏膜炎性反应的作用机制。方法:SD大鼠随机分为正常组、模型组、穴位注射组和非经非穴组,每组7只。采用卵清蛋白致敏法建立变应性鼻炎大鼠模型,穴位注射组采用双侧“迎香”和“印堂”穴位注射地塞米松和1%利多卡因混合液0.05 mL进行干预,非经非穴组于非经非穴点注射作对照,隔3 d治疗1次,共4次。叠加量化计分法评估大鼠鼻部过敏症状;HE染色法观察鼻黏膜病理形态学变化;ELISA法检测血清组胺(HA)、白细胞介素(IL)-6、IL-8、肿瘤坏死因子α(TNF-α)含量;实时荧光定量PCR法检测鼻黏膜MUC5AC mRNA的表达;Western blot法检测鼻黏膜p38 MAPK、p-p38 MAPK、MUC5AC蛋白的表达。结果:与正常组比较,模型组大鼠鼻部症状积分升高(P<0.01),血清HA、IL-6、IL-8、TNF-α含量,鼻黏膜p38 MAPK、p-p38 MAPK蛋白,MUC5AC mRNA及蛋白表达均明显升高(P<0.01)。与模型组和非经非穴组比较,穴位注射组大鼠鼻部症状积分降低(P<0.01),血清HA、IL-6、IL-8、TNF-α含量及鼻黏膜p38 MAPK、p-p38 MAPK蛋白,MUC5AC mRNA及蛋白表达均明显降低(P<0.01,P<0.05)。结论:穴位注射可减少变应性鼻炎大鼠鼻部过敏症状和MUC5AC的分泌,减轻鼻黏膜炎性反应,其作用机制可能与穴位注射抑制p38 MAPK磷酸化从而减少炎性因子释放有关。

关键词： 难治性支原体肺炎   病变区域容积   病变质量   中性粒细胞与淋巴细胞比值   D-二聚体  

摘要： 目的 分析胸部CT联合中性粒细胞与淋巴细胞比值(NLR)以及D-二聚体在难治性支原体肺炎(RMPP)患儿中的评估价值。方法 随机选取2022年3月—2023年3月永康市妇幼保健院收治的54例RMPP患儿作为观察组,另选取同期66例健康儿童作为对照组。两组儿童均接受胸部CT与NLR、D-二聚体检测,记录两组儿童CT定量参数[病变区域容积(LV)、病变质量(LQ)],根据中性粒细胞与淋巴细胞水平计算NLR,采用酶联免疫分析法检测D-二聚体水平。对比两组儿童LV、LQ、NLR、D-二聚体水平。采用多因素logistic回归分析法分析LV、LQ、NLR、D-二聚体和RMPP的相关性。运用受试者工作特征(ROC)曲线评估胸部CT联合NLR、D-二聚体对RMPP的诊断价值。结果 观察组患儿LV、LQ、NLR、D-二聚体水平均明显高于对照组,差异均有统计学意义(均P<0.05)。多因素logistic回归分析结果显示:LV、LQ、NLR及D-二聚体水平升高均是RMPP的危险因素(均P<0.05)。ROC曲线分析结果提示:LV、LQ、NLR与D-二聚体联合诊断RMPP的曲线下面积(AUC)为0.980,明显高于各指标单独检测(0.875、0.867、0.835、0.866),差异均有统计学意义(均P<0.05)。结论 胸部CT与NLR、D-二聚体对RMPP具有较高的诊断价值,且三者联合检测的诊断价值更高,可有效提高诊断灵敏度和特异度,为临床诊治提供参考。

关键词： Serum IL-6   SDF-1   CD64   early postoperative infection after limb fractures   diagnosis  

摘要： Objective. To explore the application value of combined detection of serum interleukin-6 (IL-6), stromal cell-derived factor-1 (SDF-1) and neutrophils CD64 (CD64) in the diagnosis of early postoperative infection after limb fractures. Methods. 419 patients with limb fractures un erwent surgical treatment in our hospital were selected as study subjects, and they were divided into infection group (n = 104) and non-infection group (n = 315) according to postoperative pathological results of puncture to detect the levels of serum IL-6, SDF-1 and CD64 in the two groups on the 1st, 3rd, 5th and 7th day after surgery, and to analyze the clinical diagnostic efficacy of single and combined detection of the three indexes in early postoperative infection after limb fractures by ROC curve. Results. The levels of serum IL-6, SDF-1 and CD64 in infection group were overtly higher than those in non-infection group at different time after surgery (P < 0.05), and the AUC value, specificity and sensitivity of combined detection were higher than those of single diagnosis. In this study, 14 patients in infection group underwent reoperation, 22 patients received conservative treatment in the later period, 6 patients had postoperative muscular dystrophy, and the remaining patients had a good prognosis. Conclusion. Serum IL-6, SDF-1 and CD64 are closely related to the occurrence of early postoperative infection after limb fractures, and their combination is helpful to improve diagnostic accuracy of early postoperative infection and provide effective reference value for the treatment of postoperative infection in orthopedics.