医学免疫学-文献订阅-杭州医学院图书馆

Obstructive sleep apnea in lean Vietnamese patients with type 2 diabetes: Role of FTO and TNF genetic variants

Thu Pham Minh Vo Bao The Nguyen Duyen Huynh Thien Nguyen Thach Ngoc Truong Nga Thi Ngoc Pham Quynh Xuan Tran Long Ngoc Thanh Nguyen Trung Quoc Trat Quyen Tran Xuan Phan Phuong Minh Nguyen Kien Trung Nguyen Hung Viet Phan Phu Nguyen Trong Tran

Faculty of Medicine Can Tho University of Medicine and Pharmacy Can Tho City Viet NamUniversity of Medicine and Pharmacy Hue University Hue City Viet NamCan Tho University of Medicine and Pharmacy Hospital Can Tho City Viet NamFaculty of Medicine Chulalongkorn University Bangkok Thailand

来源 PubMed期刊详细信息

关键词： Airway structure Genetic risk factor Inflammatory gene Metabolic pathway Risk prediction model Sleep disorder

摘要：

Obstructive sleep apnea is a prevalent condition in patients with type 2 diabetes, including those with normal body weight, suggesting underlying mechanisms beyond obesity. This cross-sectional study aimed to examine the association of two genetic variants, fat mass and obesity-associated (FTO) rs9939609 and tumor necrosis factor (TNF) rs1800629, with obstructive sleep apnea in lean Vietnamese patients with type 2 diabetes. A total of 130 adults with type 2 diabetes and body mass index below 25 kg per square meter underwent clinical evaluation, sleep assessment using respiratory polygraphy, and genotyping. Obstructive sleep apnea was diagnosed in 61 participants (46.9 %). The risk allele A of the FTO variant was present in 17.2 % of the obstructive sleep apnea group, compared to only 0.7 % in the non-obstructive sleep apnea group (p < 0.001). Carriers of the A allele had a 27.8-fold increased risk of obstructive sleep apnea (95 % confidence interval: 3.6 to 214.0), independent of age, sex, body mass index, neck circumference, years of diabetes, and airway anatomy. Although the TNF variant was not significantly associated with the presence of obstructive sleep apnea (odds ratio = 2.2, 95 % confidence interval: 0.7 to 7.1, p = 0.651), it showed noticeable association with apnea-hypopnea index and oxygen desaturation metrics (p-values <0.01). Both variants were significantly associated with unfavorable upper airway characteristics, including increased Mallampati grade and neck circumference. Adding the FTO genotype to standard screening tools improved diagnostic accuracy, with the area under the curve rising from 0.92 to 0.95 and net reclassification improvement reaching 50 %. These findings support the role of metabolic and inflammatory pathways in disease risk among lean patients.

Obstructive sleep apnea is

嵌合抗原受体T细胞产品生产工艺研究进展

唐浩郭晓冰陈小春杨天一

江苏太平洋美诺克生物药业股份有限公司常州213004

来源

维普期刊数据库

中国学术期刊网络出版...

万方期刊详细信息

Relaxin-2 counteracts TNF-α-induced senescence in human primary chondrocytes by enhancing telomerase activity and modulating SIRT1/p53 signaling

Jinfeng Pei Guohui Wang Minwei Yang Liwei Liu

Department of Pain Management The First Hospital of Shanxi Medical University Taiyuan Shanxi 030001 China

来源 PubMed期刊详细信息

关键词： Relaxin-2 SIRT1 cellular senescence human primary chondrocytes osteoarthritis p53 telomerase

摘要：

The pro-inflammatory cytokine TNF-α plays a crucial role in promoting cellular senescence in chondrocytes, contributing to the pathological progression of osteoarthritis (OA). Relaxin-2, a biologically active peptide hormone with diverse effects, has been investigated for its potential protective role against TNF-α-induced cellular senescence in human primary chondrocytes. In this study, human primary chondrocytes were exposed to TNF-α (10 ng/mL) with and without the presence of recombinant human relaxin-2 (rh relaxin-2). SA-β-gal staining indicated that rh relaxin-2 effectively mitigated TNF-α-induced cellular senescence in these cells. Furthermore, rh relaxin-2 enhanced telomerase activity and prevented cell cycle arrest at the G0/G1 phase induced by TNF-α. Additionally, rh relaxin-2 reduced the expression levels of plasminogen activator Inhibitor-1 (PAI-1) and p21, key regulators of cellular senescence. Interestingly, TNF-α increased K382 acetylation of p53 but decreased SIRT1 expression. Notably, knocking down SIRT1 negated the protective effects of rh relaxin-2 on cellular senescence, suggesting that SIRT1 is involved in mediating the protective effects of rh relaxin-2. These findings provide new insights into the potential therapeutic use of rh relaxin-2 for OA treatment through a novel mechanism.

The pro-inflammatory cytokine TNF-α plays a crucial role in promoting cellular senescence in chondrocytes, contributing to the pathological progression of osteoarthritis (OA). Relaxin-2, a biologically active peptide hormone with diverse effects, has been investigated for its potential protective role against TNF-α-induced cellular senescence in human primary chondrocytes. In this study, human primary chondrocytes were exposed to TNF-α (10 ng/mL) with and without the presence of recombinant human relaxin-2 (rh relaxin-2). SA-β-gal staining indicated tha

Factors influencing length of hospital stay among psychiatric inpatients in Montreal, Canada: The role of insulin, IL-6 and TNF-Alpha

Bahram Armoon Alain Lesage Charles-Édouard Giguère Keith Perry Signature Consortium Robert-Paul Juster Stéphane Guay

Institut universitaire en sante mentale de Montreal Centre integre universitaire de sante et service sociaux Est Montreal CanadaDépartement de Psychiatrie et d’Addictologie Faculté de Médecine Université de Montréal Montreal CanadaÉcole de Criminologie Faculté des Arts et des Sciences Université de Montréal Montreal Canada

来源 PubMed期刊详细信息

关键词： Insulin Interleukin-6 Psychiatric disorders Tumor Necrosis Factor Alpha length of stay

摘要：

Hospital length of stay (LOS) is a widely used indicator of healthcare efficiency and a key metric for evaluating the performance of psychiatric services. This study aims to identify predisposing, needs-related, and enabling factors associated with LOS among patients with psychiatric disorders admitted through the psychiatric emergency room in Montreal, Canada. A total of 600 individuals with mental health disorders who were hospitalized between 2012 and 2018 were interviewed. Guided by the Behavioral Model for Vulnerable Populations, factors influencing LOS were assessed using a negative binomial regression model. Longer LOS was associated with patients aged 60 and older, those diagnosed with psychotic and/or personality disorders at admission, individuals with elevated interleukin-6 (IL-6) levels (a pro- and anti-inflammatory cytokine), and those with a history of hospitalization. In contrast, shorter LOS was observed among patients with common mental health symptoms, substance use disorders (SUD), suicidal ideation, among those with elevated levels of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) or insulin. Targeted interventions such as Assertive Community Treatment for patients with personality disorders, intensive home care for those with psychotic disorders, and crisis resolution teams for individuals experiencing suicidal ideation or common mental health symptoms may help reduce hospitalization durations. For patients with SUD, improved referral systems and harm reduction–oriented outpatient care are recommended. Circulating biomarkers such as IL-6, TNF-α, and insulin may aid in early identification of patients at risk for prolonged stays and hold promise for integration into clinical decision-making and treatment. Further research is needed to validate these findings across diverse psychiatric populations.

Hospital length

HLA-specific platelet trasfusion as part of desensitization strategy in patients with donor-specific anti-HLA antibodies before stem cell transplantation

Giuliana Lando Roberto Crocchiolo Giorgia Cornacchini Giulia Di Maggio Cristina Garanzini Giovanni Grillo Benedetta Mazzi Silvano Rossini Michela Tassara

Servizio di Immunoematologia e Medicina Trasfusionale ASST Grande Ospedale Metropolitano Niguarda Milano ItalyServizio di Immunoematologia e Medicina Trasfusionale IRCCS Ospedale San Raffaele Milano Italy

来源 PubMed期刊详细信息

关键词： Anti-HLA Desensitization Donor-Specific Antibodies Mismatched Donors Platelet Transfusion

摘要：

The presence of donor-specific anti-HLA antibodies (DSAs) in patients who are candidates for allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a recognized risk factor for delayed engraftment and mortality. In the absence of an alternative donor, patient desensitization is indicated to reduce or eliminate DSAs; however, harmonization among transplant centers remains insufficient and reduction of DSAs is sometimes unsuccessful. Here, we present the feasibility and results of our recent monocenter experience on nine immunized HLA class I DSA+ patients undergoing pre-transplant desensitization using HLA-specific platelet transfusion, as a means of antibody adsorption through platelets expressing the same HLA specificities targeted by DSAs. All allo-HSCTs were from HLA-mismatched donors, both unrelated or related haploidentical. The approach appeared safe for donors and patients and potentially useful in mitigating the detrimental impact of class I DSAs, with successful engraftment observed in two of four patients with MFI > 10,000 and in all patients with MFI <10,000. Until harmonized and more risk-adapted desensitization strategies become available, sharing inter-center experiences between clinical and transfusion units will likely improve the management of hyperimmunized patients and enhance the availability of emerging desensitizing strategies.

The presence of donor-specific anti-HLA antibodies (DSAs) in patients who are candidates for allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a recognized risk factor for delayed engraftment and mortality. In the absence of an alternative donor, patient desensitization is indicated to reduce or eliminate DSAs; however, harmonization among transplant centers remains insufficient and reduction of DSAs is sometimes unsuccessful. Here, we present the feasibility and results of o

造血干细胞动员:不同方案的优缺点及预测模型与技术提升

韩孟君许芳

电子科技大学医学院四川省成都市611731电子科技大学医学院附属绵阳医院绵阳市中心医院四川省绵阳市621000

来源

维普期刊数据库

中国学术期刊网络出版...

万方期刊详细信息

关键词： 造血干细胞造血干细胞移植造血干细胞动员细胞因子血小板生成素 CXCR4拮抗剂整合素拮抗剂化疗动员效能

摘要： 背景:造血干细胞移植是一种广泛应用于血液系统恶性和非恶性疾病的重要治疗手段,动员和收集足够的造血干细胞是保证患者移植后快速、持续造血功能重建的前提,但最常用的粒细胞集落刺激因子联合或不联合化疗的经典方案仍有10%-40%的动员失败率。目的:综述近年来造血干细胞动员现状,分析不同方案的优缺点,展望未来动员新方案。方法:以“造血干细胞,造血干细胞移植,造血干细胞动员,细胞因子,血小板生成素,CXCR4拮抗剂,整合素拮抗剂,化疗,动员效能”为中文关键词,以“hematopoietic stem cell,hematopoietic stem cell transplantation,hematopoietic stem cell mobilization,cytokines,thrombopoietin,CXCR4 antagonists,integrin antagonist,chemotherapy,mobilization efficiency”为英文关键词,在CNKI、PubMed、万方数据库进行检索,文献语种限制为中文、英文,检索年限为1990-2024年,共检索到300多篇文献,最终纳入68篇文献进行分析。结果与结论:越来越多的研究发现粒细胞集落刺激因子联合普乐沙福、白细胞介素、血小板生成素及整合素拮抗剂等能够更加有效地动员造血干细胞,联合使用可以减少粒细胞集落刺激因子的使用剂量,减少相关不良反应。一些新药如可溶性重组flt3-配体(简称CDX-301)、双重α9β1/α4β1整合素抑制剂BOP等可与粒细胞集落刺激因子联合,促进造血干细胞动员。此外还发现一些潜在的动员靶点如前列腺素E2受体及1-磷酸鞘氨醇等,尚在研究阶段。除固有的患者特征、治疗特征外,开发纳入生物标志物的预测模型,有助于有效预测造血干细胞动员失败,提升干细胞动员技术。

Unravelling the potential of small molecule tumor necrosis factor-α (TNF) inhibitors: A comprehensive review

Chauhan, Yaman Kumawat, Akshay Singh, Abhay Shankaraiah, Nagula Kaki, Venkata Rao

Natl Inst Pharmaceut Educ & Res Dept Chem Sci Hyderabad 500037 India

来源 ScienceCitationIndex... PubMed期刊详细信息

关键词： TNF-alpha Small molecules Signaling transduction pathway Anti-inflammatory Biological activity In silico screening

摘要： TNF-alpha is a key cytokine, plays a critical role in the pathogenesis of autoimmune and inflammatory diseases such as rheumatoid arthritis and psoriasis. As a result, TNF-alpha has emerged as a pivotal therapeutic target. Currently, monoclonal antibodies and decoy receptors targeting TNF-alpha are widely used, yet they face limitations such as administration challenges. These drawbacks have driven the exploration of small molecule inhibitors as alternative therapeutic agents due to their oral bioavailability, ability to cross biological barriers, and cost-effective production. Significant efforts have been directed toward developing small-molecule TNF-alpha inhibitors, which function either by disrupting the TNF trimer or by blocking TNF-alpha TNFR interactions. Several candidates have progressed to clinical trials, demonstrating their potential as therapeutic agents. This review provides a comprehensive overview of TNF-alpha small-molecule inhibitors, covering their discovery, design strategies, structure-activity relationships, and biological evaluation. Additionally, it discusses current clinical outcomes and outlook to guide researchers in the drug design and development of more potent inhibitors.

KIF2C regulates macrophage M2 polarization and DLBCL progression by regulating the STAT3/IL-10 axis

Li Qian Rongfeng Shi Juanjuan Yang Jianguo Zhang

Department of Pathology Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province ChinaDepartment of Interventional & Vascular Surgery Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China

来源 PubMed期刊详细信息

关键词： KIF2C Diffuse large B-cell lymphoma Macrophage polarization Tumor progression

摘要：

KIF2C is an oncogene highly expressed in various malignancies, but its role in diffuse large B-cell lymphoma (DLBCL) remains unclear. In this study, THP-1 cells were stimulated to differentiate into M0, M1, and M2 macrophages using the culture medium of OCI-LY3 DLBCL cells. KIF2C expression was upregulated during M2 polarization. Knockdown or overexpression of KIF2C in THP-1 cells revealed that KIF2C promoted M2 but not M1 polarization. Whether in the co-culture of THP-1_M0 cells with altered KIF2C levels and OCI-LY3 cells, or in OCI-LY3 cells with altered KIF2C levels. KIF2C knockdown inhibited OCI-LY3 proliferation, migration, and invasion, and promoted apoptosis. In contrast, KIF2C overexpression enhanced M2 polarization and tumor-promoting behaviors. Mechanistically, KIF2C knockdown reduced p-STAT3 (Tyr705) levels in macrophages. Application of STAT3 agonist colivelin TFA restored IL-10 expression and M2 polarization. KIF2C knockdown in tumor cells inhibited their growth and viability, both in vitro and in a subcutaneous xenograft model, in which M2 macrophage polarization, IL-10 expression, and tumor progression were reduced. These findings suggest that KIF2C in macrophages and/or tumor cells regulates DLBCL-associated M2 macrophage polarization and tumor progression through the STAT3/IL-10 axis, highlighting KIF2C as a potential therapeutic target in DLBCL.

KIF2C is an oncogene highly expressed in various malignancies, but its role in diffuse large B-cell lymphoma (DLBCL) remains unclear. In this study, THP-1 cells were stimulated to differentiate into M0, M1, and M2 macrophages using the culture medium of OCI-LY3 DLBCL cells. KIF2C expression was upregulated during M2 polarization. Knockdown or overexpression of KIF2C in THP-1 cells revealed that KIF2C promoted M2 but not M1 polarization. Whether in the co-culture of THP-1_M0 cells with

First-trimester prediction of gestational diabetes mellitus using resistin, chemerin, progranulin, omentin, and IL-10

Huriye Ezveci Sukran Dogru Fikriye Karanfil Yaman Pelin Bahçeci Hatice Bedia Yildiz Meltem Uyaner Kan İbrahim Kilinç Kazım Gezginç

Necmettin Erbakan University Medical School of Meram Department of Obstetrics and Gynecology Division of Perinatology Konya. TurkeyKonya City Hospital Department of Obstetrics and Gynecology Division of Perinatology Konya. TurkeyNecmettin Erbakan University Medical School of Meram Department of Obstetrics and Gynecology Konya. TurkeyNecmettin Erbakan University Medical School of Meram Department of Medical Biochemistry Konya. Turkey

来源 PubMed期刊详细信息

关键词： Chemerin IL-10 first trimester gestational diabetes mellitus omentin progranulin resistin

摘要：

Objective

The study aims to evaluate the prediction of gestational diabetes mellitus (GDM) by proinflammatory resistin, chemerin, and progranulin, as well as anti-inflammatory adipokines omentin and IL-10, in maternal serum during the first trimester.

Material method

This prospective case-control study was performed in the obstetric unit of the university hospital from January 2023 to July 2024. Maternal serum samples from the study cases were collected during the first-trimester screening test to analyze resistin, chemerin, progranulin, omentin, and IL-10 levels. The cases were subsequently categorized into groups diagnosed with GDM and those not diagnosed between 24 and 28 weeks. Clinical and laboratory assessments were conducted.

Results

The study included a total of

TNF-α x FcαRI bi-specific antibody potentiates neutrophil-mediated anti-tumor effects

Gout, D.Y. Sewnath, C.A.N. Duru, G. van der Mast, R. Tuk, C.W. Bentlage, A.E.H. Gruijs, M. Vidarsson, G. Heemskerk, N. van Egmond, M.

Molecular Cell Biology and Immunology Amsterdam UMC location Vrije Universiteit Amsterdam De Boelelaan Amsterdam 1117 NetherlandsCancer Center Amsterdam Cancer Biology and Immunology Amsterdam NetherlandsAmsterdam Institute for Immunology and Infectious Diseases Immunology Amsterdam NetherlandsImmunoglobulin Research Laboratory Sanquin Research Amsterdam NetherlandsDepartment of Biomolecular Mass Spectrometry and Proteomics Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research Utrecht University Utrecht NetherlandsSurgery Amsterdam UMC Location Vrije Universiteit Amsterdam De Boelelaan Amsterdam 1117 Netherlands

来源 PubMed期刊详细信息

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

课程文献中心更多>>