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关键词： 新型冠状病毒抗原   检测试剂   企业参考品  

摘要： 目的:构建一种针对企业试剂产品的新型冠状病毒抗原检测用参考品,以实现对试剂的质量控制和性能评估。方法:以中国食品药品检定研究院制备的新型冠状病毒抗原检测试剂国家参考品为对照,构建用于验证新型冠状病毒抗原试剂检出性能的企业参考品,对两种参考品进行比较,并对参考品的稳定性进行验证。结果:企业参考品最低检出限S5标定结果为1.25×10^(5) U/mL;国家参考品与企业参考品对应的阴性参考品N1~N20、阳性参考品P1~P8及重复性参考品R1~R3检测结果色阶一致且符合要求;37˚C保存4d,-20℃保存1年或反复进行4次冻融后均不会对企业参考品的稳定性构成影响;试剂的性能验证结果表明,阴性符合率、阳性符合率、重复性结果均满足对应要求。结论:制备的企业参考品性能达到应用要求,可用于对试剂质量的控制和评价。

关键词： 肛周脓肿   病原菌分布   MMP-2   IL-10   CRP  

摘要： 目的分析肛周脓肿患者病原菌分布特征及其与血清基质金属蛋白酶-2(MMP-2)、白细胞介素-10(IL-10)、C反应蛋白(CRP)水平的相关性。方法回顾性分析2022年5月至2024年2月首都医科大学附属北京中医医院收治的101例肛周脓肿患者,记录病原菌检测分布特点;分析肛周脓肿患者、不同单一病原菌感染患者血清MMP-2、IL-10、CRP阳性检测结果;分析不同期肛周脓肿患者血清MMP-2、IL-10、CRP阳性情况。结果101份标本中有97份标本培养出细菌,阳性率为97.04%(97/101),共分离出103株细菌;97份标本中有3份标本各培养出3种细菌,混合感染率为3.09%,其余94份标本均为单一感染。肛周脓肿患者血清CRP检出阳性率76.23%(77/101)均高于MMP-282.18%(83/101)、IL-1084.15%(85/101)检出阳性率,但差异无统计学意义(χ^(2)=0.343,P>0.05)。94份单一感染样本中检出94株病原菌,其中血清MMP-2、IL-10、CRP三项指标分别在肺炎克雷伯菌、大肠埃希菌及其他细菌感染患者中的检出阳性情况比较,差异无统计学意义(P>0.05)。血清MMP-2、IL-10、CRP三项指标分别在瘘管形成期、脓肿形成中期、急性炎症期中的检出阳性情况比较,差异有统计学意义(P<0.05)。结论肛周脓肿患者主要病原菌以革兰阴性菌为主,可通过监测血清MMP-2、IL-10、CRP水平变化,为临床制定肛周脓肿患者治疗方案提供参考资料。

关键词： 复发性流产   VEGF   IGF-1   IL-6   IL-18  

摘要： 目的观察复发性流产(RSA)患者血清中血管内皮生长因子(VEGF)、血清胰岛素生长因子-1(IGF-1)、白介素-6(IL-6)、白介素-18(IL-18)的表达水平,并探讨其在RSA中的作用。方法将40例确诊为RSA的患者作为观察组,另选取40名同期正常孕妇为对照组。采用ELISA实验观察血清VEGF、IGF-1、IL-6、IL-18的表达量,并分析其与临床参数的关系及VEGF的表达与IGF-1、IL-6、IL-18的关系。结果VEGF、IGF-1、IL-6、IL-18在RSA患者血清中明显降低,与年龄、孕周、孕次均无明显差异(P>0.05);VEGF的低表达与IGF-1、IL-6、IL-18的表达水平相关(P<0.05),VEGF的低表达水平与IGF-1(r=0.307,P=0.027)、IL-6(r=0.464,P=0.001)、IL-18(r=0.344,P=0.015)的低表达水平呈正相关。结论RSA患者血清中VEGF、IGF-1、IL-6、IL-18明显降低,VEGF的低表达参与了RSA的发生,且与IGF-1、IL-6、IL-18的作用相关。

关键词： 脑卒中   抑郁   艾司西酞普兰   认知功能   神经功能  

摘要： 目的探讨采用艾司西酞普兰治疗对脑卒中后抑郁患者心理状态、神经功能及血清细胞因子的影响,为该疾病的临床治疗提供参考。方法选取2022年1月至2024年1月苏州倍磅康复医院收治的81例脑卒中后抑郁患者,根据随机数字表法分为对照组(40例,采用抗血小板聚集、神经保护等常规治疗)和观察组(41例,采用常规治疗+艾司西酞普兰治疗)。两组患者均治疗3个月。比较两组患者治疗3个月后的临床疗效,治疗前和治疗3个月后的汉密尔顿抑郁量表(HAMD)、简易精神状态检查量表(MMSE)、中国卒中量表(CSS)评分及血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、5-羟色胺(5-HT)、神经生长因子(NGF)水平,以及治疗期间的不良反应发生情况。结果治疗3个月后,观察组患者的临床疗效高于对照组;与治疗前比,治疗3个月后两组患者的HAMD、CSS评分及血清IL-6、TNF-α水平均降低,且观察组均低于对照组,MMSE评分及血清5-HT、NGF水平均升高,且观察组均高于对照组(均P<0.05),但治疗期间两组患者的不良反应总发生率比较,差异无统计学意义(P>0.05)。结论相较于单一常规治疗,在脑卒中后抑郁患者中进行艾司西酞普兰联合治疗,临床疗效显著,对患者的心理状态、认知功能、神经功能均具有改善作用,且有助于减轻机体炎症反应,同时安全性良好。

摘要： BACKGROUND: Antibodies directed against donor-specific HLA allele(s)/antigen(s) (DSAs) represent a known risk factor for hematopoietic stem cell transplantation (HSCT) engraftment. Still, the overall management needs to be standardized. MATERIAL AND METHODS: GITMO and AIBT ran a survey on DSAs in Italian Transplant Programs including mismatched HSCT performed between January 2014 and June 2017. RESULTS: One-thousand-thirty-three patients were proposed for the study, 804 were evaluable. Overall, 355 (44%) were screened: 91/355 (25.6%) showed anti-HLA antibodies, 23 DSAs (6.5%). Female gender and at least 4 previous pregnancies showed an impact on alloimmunization. Eleven patients with DSAs underwent desensitization. In seven cases no desensitization was employed. An alternative donor was selected for five patients. Neutrophil and platelet engraftment were obtained in 93.6% and 86.6% of the whole population, respectively, and were statistically associated with the absence of anti-HLA antibodies, ABO match, a higher number of infused nucleated cells and lack of a-GvHD. In addition, significant factors for platelet engraftment were the use of leuco-depleted transfusions, HLA match, younger age of the patient. Graft failure (GF) was associated with bone marrow stem cell source, and a lower number of infused CD34+. The detection of antibodies directed against both HLA classes, donor and patient age, the hematologic and molecular remission at HSCT, HLA match, ANC and PLTS engraftment, full donor engraftment within 28 days after HSCT, early and late GF, grade>II a-GVHD showed an impact on OS. DISCUSSION: Anti-HLA antibodies and DSAs were confirmed as risk factors affecting OS. DSAs were managed with various approaches resulting in stable engraftment in 81.9% of patients. Our study supports the clinical relevance of DSAs detection and management in mmHSCT. A standardized approach of DS is warranted.

关键词： 白细胞介素-6   白细胞介素-10   血流感染   病原体分类  

摘要： 目的:应用受试者工作特征曲线(ROC)定量分析白细胞介素(IL)-6、IL-10以探讨其在识别细菌血流感染(BSI)革兰染色分类中的价值。方法:测定52例明确BSI患者IL-6与IL-10值,对其进行ROC分析。结果:革兰染色阴性组(GN-BSI)的血清IL-6和IL-10水平高于革兰染色阳性组(GP-BSI)。两组比较,IL-6:敏感度为58.3%,特异性为81.2%,P=0.016,最佳截断值为642.6 pg/ml,曲线下面积(AUC)为0.73;IL-10:敏感度为59.3%,特异性为93.7%,P=0.001,最佳截断值为21.89 pg/ml,AUC为0.80;联合检测:敏感度为81.3%,特异性为72.2%,P<0.001,AUC为0.81。结论:IL-6、IL-10联合检测是区分细菌BSI革兰染色分型有价值的生物标志物。

关键词： 天门冬汤   敷贴   髓源抑制性细胞   肺癌原位模型   肿瘤外治  

摘要： 目的研究天门冬汤敷贴(以下均称TD敷贴)对肺癌原位模型小鼠的治疗作用及作用机制。方法依据课题组前期构建的小鼠肺癌原位模型方法,在小鼠左侧肺叶内注射荧光素酶标记的小鼠Lewis肺癌细胞(Lewis lung carcinoma,LLC-luc)建立小鼠肺癌原位模型。将造模小鼠随机分为对照组、TD敷贴组;分别在小鼠背部以肺俞穴为中心,0.5 cm×0.5 cm为范围贴敷敷贴,对照组贴敷空白敷贴,TD敷贴组贴敷含药敷贴,每日更换敷贴,连续治疗25 d。观察小鼠体征,记录小鼠体质量及生存期。流式细胞术检测小鼠肿瘤内主要免疫细胞的变化。通过网络药理学预测天门冬汤药物作用于髓源抑制性细胞(myeloid-derived suppressor cells,MDSCs)的潜在靶点。实时荧光定量聚合酶链式反应(Reverse Transcription-Polymerase Chain Reaction,RT-qPCR)检测小鼠白细胞介素-1β(interleukin-1β,IL-1β)、信号转导和转录活化因子3(signal transduction and transcription activator 3,STAT3)、精氨酸酶(arginase 1,Arg1)、一氧化氮合酶(inducible nitric oxide synthase,iNOS)、S100钙结合蛋白A9(S100 calcium binding protein A9,S100A9)基因表达。蛋白免疫印迹(Western blot)法检测小鼠Arg1、iNOS、S100A9、STAT3、p-STAT3蛋白表达。结果TD敷贴治疗可延长肺癌小鼠生存期(P<0.05);TD敷贴对小鼠体质量无直接影响(P>0.05);TD敷贴治疗后肺癌小鼠肿瘤中MDSCs显著降低(P<0.001),T细胞数量增加(P<0.05),其余免疫细胞如自然杀伤细胞、巨噬细胞、树突状细胞、调节性T细胞无明显变化(P>0.05)。网络药理学预测天门冬汤药物靶点通过IL-1β靶向MDSCs发挥作用。TD敷贴治疗后与MDSCs活化相关的Arg1、iNOS、S100A9 mRNA及蛋白表达水平显著降低(P<0.01);IL-1β、STAT3 mRNA表达降低(P<0.01);STAT3、p-STAT3及MDSCs活化相关的Arg1、iNOS、S100A9蛋白表达下调(P<0.05)。结论天门冬汤敷贴通过IL-1β/STAT3信号通路抑制MDSCs的增殖与活化从而延长肺癌荷瘤小鼠的生存期。

关键词： Ulcerative colitis   Abnormal immune response   γδT17 cells   NLR family pyrin domain containing 3/interleukin-1βpathway   Observational study  

摘要： BACKGROUND The interleukin-17(IL-17)mediated aberrant immune-inflammatory response plays a paramount role in ulcerative colitis(UC).γδT17 cells are one of the critical sources of IL-17,but the role they play in UC remains under *** To clarify the role ofγδT17 cells in patients with mild-to-moderate *** A single-centre observational pragmatic study was conducted on patients with UC who attended the outpatient and inpatient departments of Xiyuan Hospital of the China Academy of Traditional Chinese Medicine from September 2020 to December *** research population consisted of two groups of adult *** first group consisted of healthy volunteers with no significant abnormalities on colonoscopy,and the other group consisted of patients with mild-to-moderate ulcerative *** samples from healthy volunteers and patients with UC were collected for the detection of relevant inflammatory ***,five colon mucosa samples were randomly selected from each group for testing and *** An increased number ofγδT17 cells and hyperactivation of the NLR family pyrin domain containing 3/IL-1βsignaling pathway were observed in colonic mucosal tissues from patients with *** Hyperactivation of the NLR family pyrin domain containing 3/IL-1βsignaling pathway promotes the activation ofγδT17 cells in colonic mucosal tissues of patients with UC.

关键词： 帕金森病   骨髓基质细胞抗原1基因   多态性  

摘要： 目的 探讨骨髓基质细胞抗原1 (BST1)基因rs11931532位点、rs4698412位点多态性与帕金森病(PD)的相关性。方法 应用竞争性等位基因特异PCR(KASP)技术对新疆地区200例散发性PD(IPD)患者和197例健康对照者进行BST1基因rs11931532位点、rs4698412位点多态性分析。结果(1)在总体样本中,PD组和对照组间rs11931532的基因型分布频率比较差异均无统计学意义(P>0.05)。进一步按照性别、年龄进行分层,在男性PD组和男性对照组间、女性PD组和女性对照组间、年龄≤68岁的PD组和年龄≤68岁对照组间、在年龄>68岁的PD组和年龄>68岁对照组间,其基因型分布频率比较差异均无统计学意义(^(均)P> 0.05)。(2)在总体样本中,PD组和对照组间rs4698412基因型、等位基因频率比较差异无统计学意义(^(均)P> 0.05)。进一步按照性别、年龄进行分层,在男性PD组和男性对照组间、女性PD组和女性对照组间、年龄≤68岁的PD组和年龄≤68岁对照组间rs4698412基因型、等位基因频率比较差异无统计学意义(^(均)P> 0.05),在年龄> 68岁的PD组和年龄> 68岁的对照组间,PD组A/A基因型频率(20.4%)高于对照组(8.40%),差异有统计学意义(P=0.036),两组间的等位基因分布差异无统计学(P> 0.05)。结论 BST1基因rs11931532位点多态性不是新疆地区PD的潜在易感位点。BST1基因rs4698412位点多态性可能是新疆地区年龄> 68岁PD患者的潜在易感位点,rs4698412 A/A是其易感基因型。

关键词： genotype IL28   rs12980275   rs8099917   keratitis   herpes simplex virus type 1 (HSV-1)  

摘要： (1) Introduction: The main way of spreading the herpes simplex virus 1 (HSV-1) is through direct contact, as the virus enters the host via mucous membranes. Ocular infection can occur as a primary infection or as a recurrent one. The movement of HSV-1 along the ophthalmic branch of the fifth cranial nerve from its latency phase in the trigeminal ganglion and its activation represent a process influenced by various symbiotic factors, such as environmental conditions and the host's genetic characteristics. The aim of this study was to assess the frequency of IL28 (rs12980275) and (rs8099917) in recurrent ocular HSV infections. (2) Materials and methods: The study included 60 patients aged over 18, of both sexes, all of whom had a history of herpes simplex labialis (HSL). Patients were tested for HSV-1-specific IgG antibodies, and seropositive individuals were genotyped for single nucleotide polymorphisms (SNPs) rs12980275 and rs8099917. A total of 57 seropositive patients were included in the study. (3) Results: A statistically significant association was found between recurrent HSV keratitis (HSK) and heterozygous GT rs8099917 and homozygous TT rs8099917, as well as heterozygous AG rs12980275 and homozygous AA rs12980275 (p < 0.01). Interestingly, patients with homozygous GG polymorphism for both genotypes GG rs8099917 and GG rs12980275 did not develop recurrent HSV keratitis. (4) Conclusion: The most frequent SNP variations in patients with recurrent HSV disease were heterozygous AG rs12980275 (61.40%) and heterozygous GT rs8099917 (52.63%). Patients with recurrent HSV keratitis lacked the homozygous GG polymorphism in both GG rs8099917 and GG rs12980275 genotypes, suggesting that HSV-seropositive individuals expressing these genotypes may have lower predisposition to develop recurrent stromal HSV keratitis.