课程文献中心 更多>>

关键词： 急性淋巴细胞白血病   运动康复干预   生活质量   运动功能   心理健康  

摘要： 目的探讨急性淋巴细胞白血病患者实施运动康复干预的效果。方法选择80例急性淋巴细胞白血病患者,随机分为对照组(40例,采用常规护理干预)与实验组(40例,在常规护理干预的基础上实施运动康复干预)。对比两组运动功能(体力活动能力、肌力、耐力)评分、负性情绪[焦虑自评量表(SAS)、抑郁自评量表(SDS)]评分、生活质量评分。结果干预后,实验组体力活动能力、肌力、耐力评分分别为(90.87±5.01)、(91.52±3.01)、(86.35±2.65)分,均高于对照组的(88.13±3.26)、(89.02±4.11)、(82.35±3.11)分,差异有统计学意义(P<0.05)。实验组SAS、SDS评分分别为(32.97±3.38)、(25.97±2.08)分,均低于对照组的(35.21±2.93)、(27.71±3.33)分,差异有统计学意义(P<0.05)。实验组生理功能、生理角色、身体疼痛、一般健康状况、社交功能、情感角色评分分别为(81.37±12.31)、(73.66±13.04)、(71.97±12.08)、(81.25±14.31)、(82.63±14.25)、(83.66±13.04)分,均高于对照组的(70.02±12.12)、(61.29±12.25)、(60.51±12.33)、(69.33±12.12)、(70.69±13.13)、(71.29±14.25)分,差异有统计学意义(P<0.05)。结论急性淋巴细胞白血病患者采用运动康复干预具有明显的效果。通过运动康复干预,患者的生活质量得到提升,运动功能得到改善,心理健康得到支持。运动康复干预不仅可以帮助患者更好地应对疾病和治疗过程中的身体和心理挑战,还有助于促进患者的康复和全面恢复。

关键词： 急性呼吸窘迫综合征   患儿   前列腺素D2   白细胞相关免疫球蛋白样受体-1   诊断   预后  

摘要： 目的探讨急性呼吸窘迫综合征(ARDS)患儿血清前列腺素D2(PGD2)、白细胞相关免疫球蛋白样受体-1(LAIR-1)的表达对疾病诊断及预后的意义。方法选取2021年10月至2023年10月大冶市人民医院收治的80例2~8岁ARDS患儿为研究对象(观察组),选择同期同年龄段体检健康的94例儿童为对照组;应用酶联免疫吸附法(ELISA)检测观察组和对照组患儿血清PGD2、LAIR-1水平,采用受试者工作特征(ROC)曲线分析血清PGD2、LAIR-1对儿童发生ADRS的诊断价值;随访患儿的预后状况,并将其分为生存组65例与死亡组15例;比较观察组与对照组、生存组与死亡组的一般资料及血清PGD2、LAIR-1水平,采用多因素Logistic回归分析ADRS患儿预后的影响因素。结果观察组血清PGD2、LAIR-1水平与对照组比较,差异均有统计学意义(t值分别为7.436、7.945,P<0.05)。ROC曲线分析显示,血清PGD2、LAIR-1单独及联合诊断儿童发生ARDS的曲线下面积(AUC)分别为0.794、0.785、0.873,二者联合诊断优于PGD2、LAIR-1各自单独诊断效能(Z值分别为3.302、3.161,P<0.05)。生存组机械通气时间、小儿危重病例评分(PCIS)、氧合指数(OI)及血清PGD2和LAIR-1水平与死亡组比较,差异均有统计学意义(t值分别为6.921、2.620、15.325、2.686、5.225,P<0.05)。多因素Logistic回归分析显示,PGD2(OR=2.335,95%CI:1.713~3.183)、LAIR-1(OR=2.562,95%CI:1.451~4.523)均是ARDS患儿28d发生死亡的危险因素(P<0.05)。结论ARDS患儿血清PGD2、LAIR-1水平均有升高,二者联合检测对儿童发生ARDS具有一定的诊断价值,且与患儿的预后有关联。

关键词： 宫颈肿瘤   同步放化疗   中性粒细胞与淋巴细胞比值   癌胚抗原   预测价值  

摘要： 目的探究外周血中性粒细胞与淋巴细胞比值(NLR)和癌胚抗原(CEA)表达联合检测对局部晚期宫颈癌(LACC)同步放化疗效果的预测价值。方法选取2021年1―12月就诊于保定市第一中心医院的120例LACC病人为研究对象。根据120例LACC病人同步放化疗效果分为A组[完全缓解(CR)/部分缓解(PR),n=94]和B组[病情稳定(SD)/病情进展(PD),n=26],比较两组治疗前外周血NLR、CEA和鳞状细胞癌相关抗原(SCC-Ag)表达水平。多元logistic回归分析影响LACC病人同步放化疗效果的因素;受试者操作特征曲线(ROC曲线)分析外周血NLR和CEA表达水平对LACC病人同步放化疗效果的预测价值。结果与B组比较,A组NLR(2.51±0.24比2.97±0.36)、CEA[(6.84±0.92)μg/L比(8.77±1.03)μg/L]、SCC-Ag[(5.98±1.19)μg/L比(9.06±1.65)μg/L]表达水平明显较低(P<0.05);logistic回归分析结果显示,NLR高表达、CEA高表达、SCC-Ag高表达、淋巴结阳性、非鳞癌、FIGO分期高是影响LACC病人同步放化疗效果的独立危险因素(P<0.05)。ROC结果显示,NLR单独预测LACC病人同步放化疗效果的AUC为0.81,95%CI:(0.73,0.87),灵敏度、特异度分别为69.23%、86.17%,最佳截断值为2.96;CEA水平单独预测LACC病人同步放化疗效果的AUC为0.86,95%CI:(0.79,0.92),灵敏度、特异度分别为73.08%、85.11%,最佳截断值为8.06μg/L;SCC-Ag水平单独预测LACC病人同步放化疗效果的AUC为0.88,95%CI:(0.80,0.95),灵敏度、特异度分别为69.23%、85.11%,最佳截断值为8.28μg/L;三者联合预测LACC病人同步放化疗效果的AUC(0.94)显著大于NLR单独预测的(Z=2.61,P=0.009)、CEA单独预测的AUC(Z=2.57,P=0.010)和SCC-Ag单独预测的AUC(Z=1.99,P=0.046)。结论NLR和CEA在LACC同步放化疗有效病人中水平较低,二者联合检测对LACC同步放化疗效果具有较高的预测价值。

关键词： 梅毒   黏膜损害   临床特征   细胞免疫  

摘要： 目的分析二期梅毒患者细胞免疫水平改变情况及口腔黏膜损害临床特征,为该类患者诊断及预防干预提供参考。方法分析2021年1月至2024年3月在河南省南阳市中心医院口腔门诊就医的57例口腔黏膜损害的二期梅毒患者(观察组)人口学资料、口腔黏膜损害数目、损害部位、损害分级等临床特征。选取同期体检50例正常人为对照组,比较两组患者T淋巴细胞亚群(CD3+、CD4^(+)、CD8^(+))、NK细胞(CD56^(+))、B细胞(CD19^(+))水平及CD4^(+)/CD8^(+)比值等细胞免疫水平。结果57例口腔黏膜损害二期梅毒患者中,78.95%(45/57)表现为多部位损害。按人口学特征分类,青年组黏膜损害2~3处占比和>3处占比均高于中年组及老年组,差异有统计学意义(z=1.886,P=0.045);老年组黏膜损害Ⅲ级占比明显高于中年组及青年组,差异有统计学意义(z=1.927,P=0.041)。按损害类型分类,以黏膜斑为最多(68.42%,39/57),其次为黏膜炎(43.86%,25/57);59.65%的患者表现为2种及以上类型损害;损害好发部位依次为舌(59.65%,34/57)、唇(50.88%,29/57)、颚(38.59%,22/57)、颊部(29.82%,17/57)。细胞免疫方面:观察组患者CD3+、CD4^(+)、CD4^(+)/CD8^(+)比值、NK细胞(CD56^(+))水平明显低于对照组,差异有统计学意义(P<0.05),B细胞(CD19^(+))、T+淋巴细胞亚群中CD8水平与对照组比较差异无统计学意义(P>0.05)。结论二期梅毒患者口腔黏膜损害临床特征呈多样性;在细胞免疫方面,T淋巴细胞亚群、NK细胞显著下降,存在明显的细胞免疫抑制。

关键词： Malaria   Vaccine development   Vaccines   Major histocompatibility complex   Peptide synthesis   Immune response   Cytotoxic T cells   Enzyme-linked immunoassays  

摘要： Background A three-antigen DNA-prime/chimpanzee adenovirus 63 (ChAd63) boost vaccine containing pre-erythrocytic Plasmodium falciparum (Pf) circumsporozoite protein (CSP), Pf apical membrane antigen-1 (AMA1) and malaria multiple epitopes (ME) fused to Pf thrombospondin-related adhesion protein (ME-TRAP) elicited higher vaccine efficacy (VE) in an open label, randomized Phase 1 trial against controlled human malaria infection (CHMI) than the two-antigen vaccine DNA/Human Adenovirus 5 (HuAd5) containing CSP and AMA1. The objective of this follow-up study was to determine whether responses to CSP, AMA1 or TRAP MHC Class I-restricted epitopes were associated with VE. Methodology Protected (n = 6) and non-protected participants (n = 26) were screened in FluoroSpot interferon gamma (IFN-γ) and Granzyme B (GzB) assays using antigen-specific 15mer peptide subpools spanning CSP (n = 9 subpools), AMA1 (n = 12 subpools), and TRAP (n = 11 subpools). Individual antigen-specific 15mers in the subpools with strong responses were then deconvoluted, evaluated for activities, and MHC Class I-restricted epitopes within the active 15mers were predicted using NetMHCpan algorithms. The predicted epitopes were synthesized and evaluated in the FluoroSpot IFN-γ and GzB assays. Results Protected and some non-protected participants had similar responses to individual antigen-specific peptide subpools, which did not distinguish only protected participants. However, deconvoluted antigen-specific positive subpools with high magnitudes of responses revealed individual 15mer peptides containing specific and/or predicted MHC Class I (HLA) epitopes. Responses to epitopes were either IFN-γ-only, IFN-γ and GzB, or GzB-only. Due to limitation of cells, most of the analysis concentrated on the identification of protection associated AMA1 epitopes, since most of the predominant pool specific responses were generated against AMA1 15mer subpools. Furthermore, we previously identified protection associated

关键词： 老年   多重耐药菌   肺炎   微小RNA-127-5p   微小RNA-127   微小RNA-155   免疫因子   相关性  

摘要： 目的探究老年多重耐药菌感染肺炎患者微小核糖核酸(miR)-127-5p、miR-127、miR-155表达水平,并分析其与免疫相关细胞因子的相关性。方法选取2020年6月到2022年6月本院收治的65例老年多重耐药菌感染肺炎患者作为观察组,并选取同期健康体检人员80例作为对照A组和同期收治的老年普通肺炎患者80例作为对照B组。比较三组血清miR-127-5p、miR-127、miR-155表达水平和γ干扰素(IFN-γ)、白细胞介素(IL)-4、IL-17、转化生长因子-β(TGF-β)水平,采用Pearson相关分析血清miR-127-5p、miR-127和miR-155表达水平与免疫相关细胞因子的相关性,采用受试者工作特征曲线(ROC)分析三者单独及联合对老年多重耐药菌感染肺炎的诊断价值。结果对照B组和观察组血清miR-127-5p、IFN-γ水平低于对照A组,且观察组低于对照B组(P<0.05);而对照B组和观察组血清miR-127、miR-155、IL-4、IL-17、TGF-β水平高于对照A组,且观察组高于对照B组(P<0.05)。血清miR-127-5p表达水平与血清IFN-γ水平呈正相关(r=0.463,P<0.05),与血清IL-4、IL-17、TGF-β水平呈负相关(r=-0.378、-0.396、-0.408,P<0.05);而血清miR-127和miR-155表达水平与血清IFN-γ水平呈负相关(r=-0.267、-0.513,P<0.05),与血清IL-4、IL-17、TGF-β水平呈正相关(r=0.306、0.273、0.330和r=0.410、0.453、0.385,P<0.05)。血清miR-127-5p、miR-127和miR-155表达水平单独及联合诊断对老年多重耐药菌感染肺炎具有一定的诊断价值,其AUC值分别为0.811、0.733、0.817、0.906,敏感度为90.77%、60.00%、67.69%、84.62%,特异度为66.87%、80.62%、83.75%、83.18%。结论miR-127-5p、miR-127和miR-155在老年多重耐药菌感染肺炎患者中差异性表达,与免疫相关细胞因子具有一定的相关性,三者联合检测对老年多重耐药菌感染肺炎具有较好的诊断价值。

关键词： HLA-DPB1*1483:01   next-generation sequencing   novel allele  

摘要： We describe the novel HLA-DPB1*1483:01 allele found in a deceased Hispanic/Latino male. © 2025 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

关键词： Bifidobacterium longum subsp.infantis   Intestinal barrier interferon-beta(IFN-β)secretion   Age dependence  

摘要： Bifidobacterium longum *** is a commensal bacterium that predominates in the infant gut,playing a critical role in both preventing foreign infections and facilitating immune *** study aimed to explore the effects of *** *** supplementation on interferon-beta(IFN-β)secretion and intestinal barrier improvement in growing *** and male mice were orally administered either saline or *** *** CCFM1269 or I5TI(1×10^(9) CFU/mice per day,n=8)from 1-week-age until 3-,4-,and *** sequencing analysis revealed that CCFM1269 exhibited potential antiviral capacity through increasing 2'-5'oligoadenylate synthetase(OAS).Additionally,CCFM1269 supplementation significantly increased colonic IFN-β levels which combined with OAS in 3-week-old female and male mice by activating the TLR4-TRIF-dependent signaling ***,this effect was not observed in 4-and 5-week-old ***,both CCFM1269 were found to modulate the gut microbiota composition and enhance the intestinal barrier function in 3-,4-,and 5-week-old *** summary,the results of this study suggested that *** *** CCFM1269 promoting intestinal barrier and releasing IFN-β in growing mice was in a strain-specific and time-dependent manner.

关键词： Brazilian population   HLA‐DPB1   new allele   NGS   REDOME  

摘要： Identification of three novel HLA alleles HLA-DPB1*1619:01, -DPB1*1621:01, and -DPB1*1737:01 in Brazilian individuals. © 2025 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

摘要： The activated B-cell-like subtype of diffuse large B-cell lymphoma (ABC-DLBCL) displays a worse outcome than the germinal center B-cell-like subtype (GCB-DLBCL). Currently, targeting the tumor microenvironment (TME) is the most promising approach to cure DLBCL with profound molecular heterogeneity;however, the factors affecting the tumor-promoting TME of ABC-DLBCL remain elusive. Here, cytokine interleukin-16 (IL-16) is expressed in tumor cells of ABC-DLBCL and secreted by the cleavage of active caspase-3. The serum IL-16 levels are not only a sensitive marker of treatment response, but also positively correlated with unfavorable prognosis in DLBCL patients. While IL-16 shows few direct promotional effects on tumor cell growth in vitro, its bioactive form significantly promotes tumor progression in vivo. Mechanically, IL-16 increases the infiltration of macrophages by the chemotaxis of CD4⁺ monocytes in the TME, enhancing angiogenesis and the expression of cytokine IL-6 and IL-10, as well as decreasing T-cell infiltration to accelerate tumor progression. This study demonstrates that IL-16 exerts a novel role in co-ordinating the bidirectional interactions between tumor progression and the TME. IMM0306, a fusion protein of CD20 mAb with the CD47 binding domain of SIRPα, reverses the tumor-promoting effects of IL-16, providing new insights into treatment strategy in ABC-DLBCL. ©2025 Ferrata Storti Foundation.