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关键词： 生物陶瓷材料   牙髓复盖术   炎症反应   炎症因子表达  

摘要： 目的探讨生物陶瓷材料(iRoot BP Plus)直接盖髓术对大鼠磨牙牙髓炎症反应的调控作用,及其对炎症因子白细胞介素-6(IL-6)的时效性影响。方法建立36只SD大鼠磨牙直接盖髓模型,随机分为iRoot BP Plus组、MTA组和空白对照组,每组12只。各组大鼠术后7 d、14 d、28 d分批取材,通过HE染色评估牙髓组织炎症程度、免疫组化法检测IL-6的表达水平,并比较分析。结果各组大鼠术后7 d炎症反应程度无差异(P>0.05),IL-6阳性表达量差异有统计学意义(P<0.05),iRoot BP Plus组表达量低于其余两组;术后14 d,iRoot BP Plus组炎症反应程度低于空白对照组(P<0.05),且IL-6阳性表达量仍低(P<0.05);术后28 d,iRoot BP Plus组炎症反应程度低于空白对照组(P<0.05),与MTA组无差异(P>0.05),iRoot BP Plus组和MTA组IL-6阳性表达量无组间差异(P>0.05)。结论iRoot BP Plus能有效调控牙髓炎症进程,早期即可显著抑制IL-6表达,其抗炎效能随时间推移与MTA趋于一致,为临床盖髓材料选择提供了实验依据。

关键词： 再生障碍性贫血   中医药   免疫   细胞因子   信号通路   虫子学说  

摘要： 再生障碍性贫血(AA)是由多因素引起的骨髓造血功能减低或衰竭的血液系统疾病。AA经典发病机制与“虫子学说”密切相关,即免疫异常。多种免疫调节药物治疗AA有良好的临床疗效,说明免疫介导的造血抑制是AA发病原因之一。目前,中医药治疗AA多从干预造血细胞、改善骨髓微环境等方面探索,从免疫机制方面干预AA的研究较少。本文对近年来中医药调控免疫细胞、细胞因子和信号通路等方面治疗AA的文献进行综述,为今后深入研究AA发病机制与新治疗靶点提供证据和思路。

关键词： 人类嗜T淋巴细胞病毒   献血者   感染   随访  

摘要： 目的了解湖南省2022—2024年献血者人群中人类嗜T淋巴细胞病毒(HTLV)感染的流行情况。方法采用酶联免疫吸附试验(ELISA)对湖南省14个地州市血站近3年的1830342名献血者进行抗-HTLV-Ⅰ/Ⅱ筛查,初筛反应性标本同时进行Line Immuno Assay(LIA®)/MP-蛋白免疫印迹、RT-PCR核酸检测。对HTLV确证阳性的献血者进行追踪随访。结果2022—2024年,湖南省抗-HTLV-Ⅰ/ⅡELISA初筛阳性率为1.36/万(249/1830342);确证阳性率为0.20/万(37/1830342),确证阳性在初筛反应性献血者中占比为14.86%。HTLV确证阳性献血者随访成功率仅为18.92%,HTLV不确定献血者随访成功率为54.17%。结论岳阳、娄底、邵阳、益阳、株洲5市HTLV确认感染率高于湖南省平均水平(0.20/万)。郴州、永州、张家界、湘西4市为本省低流行区域,感染率为0。整体随访成功率较低,随访工作面临较大困难和瓶颈。HTLV全面筛查及确证随访研究,可为进一步完善血液安全检测策略和风险预警机制,提供有效数据支持。

关键词： bidirectional   cancer   cardiovascular disease   epigenetics   pathways   reverse cardio-oncology   risk factors   mechanisms   BMI   body mass index   CAD   coronary artery disease   CHIP   clonal hematopoiesis of indeterminate potential   CVD   cardiovascular disease   HDAC   histone deacetylase   HIF   hypoxia-inducible factor   IL   interleukin   miRNA   microRNA   ncRNA   noncoding RNA   PM2.5   particulate matter <2.5 μm in diameter   ROS   reactive oxygen species   TME   tumor microenvironment  

摘要： Cancer and cardiovascular disease (CVD) remain the leading causes of morbidity and mortality worldwide, with emerging evidence highlighting their complex and bidirectional interplay. Shared risk factors, including aging, systemic inflammation, metabolic dysregulation, and lifestyle behaviors, can contribute to their co-occurrence while underlying biological mechanisms such as oxidative stress, chronic inflammation, and clonal hematopoiesis further reinforce their connection. These mechanisms drive pathophysiological changes contributing to disease progression, increasing susceptibility to both conditions. This review explores the epidemiology, overlapping biological pathways, and risk factors linking cancer and CVD, emphasizing key mechanisms such as epigenetic modifications, immune system dysregulation, and cellular senescence. Future research should aim to identify biomarkers, refine risk models, and develop targeted strategies to mitigate disease burden and improve outcomes.

关键词： 糖链抗原199   癌胚抗原   糖链抗原125   甲胎蛋白   原发性肝癌   早期诊断  

摘要： 目的:探究糖链抗原199(CA199)、癌胚抗原(CEA)、糖链抗原125(CA125)及甲胎蛋白(AFP)在原发性肝癌早期诊断中的价值。方法:选取2022年1月—2024年2月于北京中医药大学东直门医院洛阳医院·洛阳市中医院就诊的原发性肝癌患者及肝硬化患者,对应为肝癌组和肝硬化组,每组各82例,另选取同期健康体检者82例作为健康对照组。比较3组研究对象血清CA199、CEA、CA125、AFP水平及单独和联合检测的阳性率,并比较4项血清指标单独及联合检测诊断原发性肝癌的灵敏度、特异度、准确度。结果:肝癌组患者血清CA199、CEA、CA125、AFP水平均明显高于肝硬化组和健康对照组,差异均有统计学意义(P<0.05);肝癌组患者4项血清指标单独及联合检测的阳性率也均明显高于肝硬化组和健康对照组,差异均有统计学意义(P<0.05);且在肝癌组中,联合检测的阳性率均明显高于单独检测;4项血清指标联合检测的诊断灵敏度和准确度均明显高于各项血清指标单独检测。结论:原发性肝癌患者血清CA199、CEA、CA125、AFP水平均明显升高,联合检测以上血清指标可有效提高原发性肝癌的检出率,具有较高的诊断灵敏度和准确度。

关键词： infectious bronchitis virus(IBV)   vaccines   recombinant fusion   antigenic epitopes   yolk antibody  

摘要： [Objective]To design and express a recombinant protein rMKIBV incorporating confirmed antigenic epitopes of infectious bronchitis virus(IBV)as a vaccine to provide comprehensive ***,it explores the potential of polyclonal yolk antibodies(IgY)harvested from laying hens immunized with the rMKIBV vaccine in the prevention and control of IBV.[Methods]The antigenic epitope sequences of IBV,obtained from online databases,were compared with sequences of representative IBV strains from *** peptides were designed to link all antigenic *** constructed amino acid sequence was analyzed,reverse-translated,codon-optimized,and then inserted into the pET-28a(+)cloning *** recombinant vector was introduced into Escherichia coli for *** purified,desalted,and endotoxin-removed rMKIBV protein was used as a vaccine to immunize animals for investigation of its immunogenicity and ability to stimulate specific IgY production in laying hens.[Results]The retrieved IBV antigenic epitope sequences showed high similarity with the published N and S protein sequences of 22 representative IBV *** predicted isoelectric point and molecular weight of rMKIBV were 10.25 and 63.39 kDa,*** secondary structure of rMKIBV included a high proportion of random coils,which suggested strong ***-purity rMKIBV was obtained from *** transformed with the recombinant plasmid *** protein specifically bound to anti-His-tag antibodies,N protein antibodies,and S protein *** mice immunized with this protein showed increases in the spleen index(P<0.05),elevations in the levels of serum-specific IgG antibodies(P<0.01)and IFN-γ(P<0.05),and no significant change in the IL-2 *** laying hens successfully produced IgY in egg yolks,with specific IgY antibody levels significantly ***,the IgY antibody titer gradually rose after immunization,reaching the peak after about 50 days and

关键词： Allergy   Contact hypersensitivity   Inflammatory skin diseases   T cells   Transcription factors   ACD   allergic contact dermatitis   ACT   adoptive cell transfer   APC   antigen-presenting cell   CHS   contact hypersensitivity   dLN   draining lymph node   TNBS   2,4,6-trinitrobenzene sulfonic acid   TNCB   2,4,6-trinitrochlorobenzene   Treg   regulatory T cell   WT   wild-type  

摘要： A plethora of data supports a major role of CD4 + and CD8 + T lymphocytes for the initiation, progression, and maintenance of allergic contact dermatitis. However, in-depth understanding of the molecular mechanisms is still limited. NFATc1 plays an essential role in T-cell activation. We therefore investigated its impact on contact hypersensitivity, the mouse model for allergic contact dermatitis. The contact hypersensitivity response to 2,4,6-trinitrochlorobenzene was diminished in Nfatc1 fl/fl xCd4-cre mice ( Nfatc1 −/− ) compared with that in wild-type mice and associated with a lower percentage of IL-17–producing CD8 + T (Tc17) cells in both inflamed skin and draining lymph nodes. In vitro Tc17 polarization assays revealed that Nfatc1 −/− CD8 + T cells have a reduced capacity to polarize into Tc17 cells. Applying single-cell RNA sequencing, we realized that NFATc1 controls the T-cell differentiation fate. In the absence of NFATc1, CD8 + T cells favor the development of IFN-γ–secreting CD8 + T (Tc1) lymphocytes, whereas in its presence, they turn into Tc17 cells. Finally, the adoptive transfer of 2,4,6-trinitrochlorobenzene–sensitized wild-type CD8 + T cells restored the contact hypersensitivity response in naïve Nfatc1 −/− mice. Our data demonstrate that NFATc1 contributes to the development of Tc17 cells and might present a promising target to alleviate CD8 + T-cell–mediated allergic responses.

关键词： 子宫内膜息肉术后   生化汤   穴位贴敷   地屈孕酮  

摘要： 目的:探讨生化汤穴位贴敷联合地屈孕酮对血瘀型子宫内膜息肉(EPs)术后患者的疗效及可能作用机制。方法:选取2023年6月—2024年7月在福建中医药大学附属第三人民医院中医妇科就诊的血瘀型EPs术后患者60例,按随机数字表法分为对照组和观察组,各30例。对照组予口服地屈孕酮片,观察组予生化汤穴位贴敷联合口服地屈孕酮片,2组共治疗3个月经周期。比较2组疗效、中医证候积分、子宫内膜厚度、月经失血图(PBAC)评分、血清转化生长因子β1(TGF-β1)、肿瘤细胞坏死因子(TNF-α)、白细胞介素-6(IL-6)含量、复发率及不良反应发生率情况。结果:治疗后,观察组总有效率高于对照组,且复发率低于对照组(P<0.05),2组不良反应发生率比较,差异无统计学意义(P>0.05)。治疗后,2组中医证候积分、子宫内膜厚度及月经PBAC评分、血清TGF-β1、TNF-α、IL-6含量均较治疗前降低(P<0.05),且观察组上述指标均较对照组降低(P<0.05)。结论:生化汤穴位贴敷联合地屈孕酮治疗血瘀型EPs术后患者,安全有效,能够改善中医证候,降低子宫内膜厚度,其可能通过降低血清TGF-β1、TNF-α、IL-6含量,减轻炎症反应,抑制息肉生长,降低复发率。

关键词： 吴茱萸碱   NLRP3   白细胞介素-1β   胱天蛋白酶1   脓毒症肝损伤  

摘要： 目的探讨吴茱萸碱(EVO)能否通过抑制NLRP3/白细胞介素-1β(IL-1β)/胱天蛋白酶1(caspase-1)信号通路减轻脓毒症大鼠的肝损伤。方法构建脓毒症肝损伤大鼠模型,将建模成功的大鼠分为模型组及L-EVO组、M-EVO组、H-EVO组(EVO灌胃4、8、16 mg/kg)、H-EVO+NLRP3组(EVO灌胃16 mg/kg+NLRP3信号通路激活剂尼日利亚菌素钠盐腹腔注射1 mg/kg),每组各10只。另取10只大鼠作为假手术组(不结扎不穿刺,其余步骤与造模大鼠相同),对照组与模型组给予等量生理盐水(1次/d,连续28 d)。检测大鼠肝功能。用HE染色观察大鼠肝组织病理变化,用TUNEL染色检测大鼠肝组织细胞凋亡,用ELISA检测大鼠血清IL-6、肿瘤坏死因子α(TNF-α)、IL-1β水平,用Western blotting检测大鼠肝组织中NLRP3/IL-1β/caspase-1信号通路蛋白表达的变化。结果与对照组相比,模型组大鼠肝组织失去正常结构,肝细胞分布不均匀,伴水肿与空泡;与模型组相比,L-EVO组、M-EVO组、H-EVO组大鼠肝组织细胞排列相对整齐,水肿与空泡减轻,且随着剂量增加,肝组织细胞形态恢复明显;与H-EVO组相比,H-EVO+NLRP3组大鼠肝组织细胞排列紊乱,可见水肿与空泡。与对照组相比,模型组大鼠丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肝组织细胞凋亡率,血清IL-6、TNF-α、IL-1β,肝组织NLRP3、IL-1β、caspase-1蛋白表达水平升高(P<0.05);与模型组相比,L-EVO组、M-EVO组、H-EVO组大鼠ALT、AST,肝组织细胞凋亡率,血清IL-6、TNF-α、IL-1β,肝组织NLRP3、IL-1β、caspase-1蛋白表达水平降低(P<0.05);与H-EVO组相比,H-EVO+NLRP3组大鼠ALT、AST,肝组织细胞凋亡率,血清IL-6、TNF-α、IL-1β,肝组织NLRP3、IL-1β、caspase-1蛋白表达水平升高(P<0.05)。结论EVO可能通过调节NLRP3/IL-1β/caspase-1信号通路减轻脓毒症大鼠肝损伤。

关键词： 糖尿病性白内障   房水   血管内皮生长因子   白细胞介素-6   转化生长因子β1   基质金属蛋白酶-9  

摘要： 目的:探讨糖尿病性白内障(DC)患者房水中血管内皮生长因子(VEGF)、白细胞介素-6(IL-6)、转化生长因子-β1(TGF-β1)、基质金属蛋白酶-9(MMP-9)水平变化及临床意义。方法:选取2022年2月—2024年2月某院收治的DC患者118例为观察组,另选取同期就诊的单纯白内障患者30例为对照组,依据DC分期将观察组分为初始期(n=34)、肿胀期(n=30)、成熟期(n=42)及过熟期(n=12),比较观察组和对照组、不同分期DC患者房水中VEGF、IL-6、TGF-β1、MMP-9水平,采用Spearman系数分析各指标与DC分期的相关性,绘制ROC曲线分析房水中VEGF、IL-6、TGF-β1、MMP-9水平对DC的诊断效能。结果:观察组房水中VEGF、IL-6、TGF-β1、MMP-9水平均高于对照组,差异有统计学意义(P<0.05)。不同DC分期患者房水中VEGF、IL-6、TGF-β1、MMP-9水平比较:初始期<肿胀期<成熟期<过熟期,差异有统计学意义(P<0.05)。相关性分析显示,房水中VEGF、IL-6、TGF-β1、MMP-9水平与DC分期呈正相关(r=0.466、0.437、0.421、0.433,P均<0.05)。房水中VEGF、IL-6、TGF-β1、MMP-9诊断DC的AUC分别为0.820、0.881、0.791、0.888;四者联合诊断的AUC为0.983,灵敏度为95.33%,特异度为96.67%。结论:DC患者房水中VEGF、IL-6、TGF-β1、MMP-9水平异常升高,且与DC分期呈正相关,联合检测其水平具有较高的诊断效能。