关键词:
magnetoencephalography
pain
sex differences
alpha activity
inflammation
摘要:
Objective Magnetoencephalography(MEG),a non-invasive neuroimaging technique,meticulously captures the magnetic fields emanating from brain electrical *** with MEG based on superconducting quantum interference devices(SQUID),MEG based on optically pump magnetometer(OPM)has the advantages of higher sensitivity,better spatial resolution and lower ***,most of the current studies are clinical studies,and there is a lack of animal studies on MEG based on OPM ***,a multifaceted sensory and emotional phenomenon,induces intricate alterations in brain activity,exhibiting notable sex *** clinical revelations of pain-related neuronal activity through MEG,specific properties remain elusive,and comprehensive laboratory studies on pain-associated brain activity alterations are *** aim of this study was to investigate the effects of inflammatory pain(induced by Complete Freund’s Adjuvant(CFA))on brain activity in a rat model using the MEG technique,to analysis changes in brain activity during pain perception,and to explore sex differences in pain-related MEG *** This study utilized adult male and female Sprague-Dawley *** pain was induced via intraplantar injection of CFA(100μl,50%in saline)in the left hind paw,with control groups receiving *** behavior was assessed using von Frey filaments at baseline and 1 h *** MEG recording,anesthetized rats had an OPM positioned on their head within a magnetic shield,undergoing two 15-minute sessions:a 5-minute baseline followed by a 10-minute mechanical stimulation *** analysis included artifact removal and time-frequency analysis of spontaneous brain activity using accumulated spectrograms,generating spectrograms focused on the 4-30 Hz frequency *** MEG recordings in anesthetized rats during resting states and hind paw mechanical stimulation were compared,before and after saline/CFA *** stimulation