关键词： alternative splicing   RNA-binding protein   transcription factor   TF-RBP-AS triplets   regulation mechanism  

摘要： Aberrant alternative splicing (AS) is increasingly linked to cancer;however, how AS contributes to cancer development still remains largely unknown. AS events (ASEs) are largely regulated by RNA-binding proteins (RBPs) whose ability can be modulated by a variety of genetic and epigenetic mechanisms. In this study, we used a computational framework to investigate the roles of transcription factors (TFs) on regulating RBP-AS interactions. A total of 6519 TF-RBP-AS triplets were identified, including 290 TFs, 175 RBPs, and 16 ASEs from TCGA-KIRC RNA sequencing data. TF function categories were defined according to correlation changes between RBP expression and their targeted ASEs. The results suggested that most TFs affected multiple targets, and six different classes of TF-mediated transcriptional dysregulations were identified. Then, regulatory networks were constructed for TF-RBP-AS triplets. Further pathway-enrichment analysis showed that these TFs and RBPs involved in triplets were enriched in a variety of pathways that were associated with cancer development and progression. Survival analysis showed that some triplets were highly associated with survival rates. These findings demonstrated that the integration of TFs into alternative splicing regulatory networks can help us in understanding the roles of alternative splicing in cancer.

关键词： Ocean   Southern Ocean   Channel flows   Forcing   Momentum   Ocean dynamics  

摘要： Topographic form stress (TFS) plays a central role in constraining the transport of the Antarctic Circumpolar Current (ACC), and thus the rate of exchange between the major ocean basins. Topographic form stress generation in the ACC has been linked to the formation of standing Rossby waves, which occur because the current is retrograde (opposing the direction of Rossby wave propagation). However, it is unclear whether TFS similarly retards current systems that are prograde (in the direction of Rossby wave propagation), which cannot arrest Rossby waves. An isopycnal model is used to investigate the momentum balance of wind-driven prograde and retrograde flows in a zonal channel, with bathymetry consisting of either a single ridge or a continental shelf and slope with a meridional excursion. Consistent with previous studies, retrograde flows are almost entirely impeded by TFS, except in the limit of flat bathymetry, whereas prograde flows are typically impeded by a combination of TFS and bottom friction. A barotropic theory for standing waves shows that bottom friction serves to shift the phase of the standing wave's pressure field from that of the bathymetry, which is necessary to produce TFS. The mechanism is the same in prograde and retrograde flows, but is most efficient when the mean flow arrests a Rossby wave with a wavelength comparable to that of the bathymetry. The asymmetry between prograde and retrograde momentum balances implies that prograde current systems may be more sensitive to changes in wind forcing, for example associated with climate shifts.

摘要： The cell wall integrity (CWI) signaling activates the transcription factor Swi6 through a MAPK signaling cascade in response to cell wall stresses. In this study, we observed two different mRNA variants of swi6 (GlSwi6A and GlSwi6B) existed, due to alternative splicing. Besides, the expression level of GlSwi6B was higher than that of the GlSwi6A mRNA variant. The co-silencing of GlSwi6A and GlSwi6B was more sensitive to cell wall stress compared with WT, resulting in a decrease of 78% and 76% in chitin and beta-1,3-d-glucan content respectively. However, only the overexpression of GlSwi6B decreased the sensitivity to cell wall stress and increased the content of chitin and beta-1,3-d-glucan compared with the WT strain. Furthermore, Y1H, EMSA and BLI assays revealed that the GlSwi6B could bind to the promoters of chitin and glucan synthesis genes (GL24454 and GL18134). However, the binding phenome has not been observed in the isoform GlSwi6A. Taken together, our results found two different transcripts generated from Swi6, in which the alternative splice isoform of GlSwi6B participates in regulating the CWI of G. lucidum. This study provides the first insight into the alternative splicing isoform of GlSwi6B in the regulation of CWI signaling in fungi.

摘要： INTRODUCTION: To describe the characteristics and outcomes of children with cystic fibrosis (CF) hospitalized with cirrhosis in the United States. METHODS: We conducted a population-based cohort study of hospitalizations among children with CF using the 2016 Kid's Inpatient Database. RESULTS: In total, 9,615 admissions were analyzed. Diagnosis of cirrhosis was present in 509 (5.3%) and was significantly associated with increased mortality, length of stay, and hospital charges compared with those without cirrhosis. Hepatic encephalopathy was significantly associated with death in children with cirrhosis. DISCUSSION: Future interventions should be designed to support children with CF who have cirrhosis to improve clinical outcomes.

关键词： urinary tract infections   arbutin   umbelliferon   N-acetyl l-cysteine  

摘要： Genito-urinary tract infections have a high incidence in the general population, being more prevalent among women than men. These diseases are usually treated with antibiotics, but very frequently, they are recurrent and lead to the creation of resistance and are associated with increased morbidity and mortality. For this reason, it is necessary to develop new compounds for their treatment. In this work, our objective is to review the characteristics of the compounds of a new formulation called Itxasol (c) that is prescribed as an adjuvant for the treatment of UTIs and composed of beta-arbutin, umbelliferon and n-acetyl cysteine. This formulation, based on biomimetic principles, makes Itxasol (c) a broad-spectrum antibiotic with bactericidal, bacteriostatic and antifungal properties that is capable of destroying the biofilm and stopping its formation. It also acts as an anti-inflammatory agent, without the adverse effects associated with the recurrent use of antibiotics that leads to renal nephrotoxicity and other side effects. All these characteristics make Itxasol (c) an ideal candidate for the treatment of UTIs since it behaves like an antibiotic and with better characteristics than other adjuvants, such as D-mannose and cranberry extracts.

关键词： Complementary and alternative medicine   Health behavior   patients' needs   Information sources   Satisfaction  

摘要： Introduction: The interest in CAM among cancer patients is constantly growing and about 50% already used CAM alongside cancer therapy. Little is known on the factors influencing patients' choice of type of CAM used. Methods: A questionnaire consisting of two validated instruments (the ASKU (Allgemeine Selbstwirksamkeit Kurzskala), the PAM 13-D (Patient Activation Measure) and the structured AKKOM questionnaire on CAM usage was distributed at a German university hospital. Results: 639 patients (male 32.9%, female 63.2%;gynecological cancer 41%, gastrointestinal 19.2%, urogenital 15.6%) took part. 60% had used CAM in the last 3 months (biological 73%, holistic 63%, mind-body-methods 62%). Participants up to 30 years preferred biologically (p = 0.001), while women with gynecological cancer favored holistic based methods (p < 0.0001). There was no association between patients' beliefs on cancer causes and the chosen CAM method. Conclusion: Improving knowledge in patients on cancer etiology and treatments could facilitate the understanding of additional complementary treatments.

关键词： Artisanal fisheries   Data-limited   Data mining   Mugil liza   Newspaper   Self-report   Self-sampling  

摘要： Assessing fish stocks harvested by small-scale fisheries is challenging. The lack of official fisheries data constrains the proper management of such fisheries. Thus, alternative sources of information are crucial to enrich data-poor fisheries. Here, we evaluated different sources of data for the mullet (Mugil liza) fishery, one of the most important but overexploited fisheries in Brazil. We gathered three alternative sources of catch data by artisanal fisheries: 14 years of self-reported catches by artisanal fishers across 24 municipalities;16 years of catches by traditional beach seines mined from news outlets;and 13 years from a single community monitoring their beach seine catches. We tested whether alternative data sources follow the same trends of landing reports from systematic, official monitoring of the industrial fleet. We fitted Bayesian time-series models to test if environmental changes and stock abundance can predict these data. We found that only self-reported catches matched the official reporting trends, thereby improving our understanding of changes in the mullet stock. These findings reveal that self-reported catches by fishers provide reliable additional data useful for management. Self-reporting data are cost-effective, deals with the complexity of small-scale fisheries, and welcomes fishers as key stakeholders in management practices.

关键词： Renewable energy   Physical process   Energy accumulator   Temperature  

摘要： The relevance of the paper appears as a social component in the formation of the structure for the development of the provision of electricity to the territory of a city or other settlement. The aim of the article is to research the electricity tariff regulation model for generation energy sources. The novelty of the study is determined by the fact that the tariff formation model for the integrated use of traditional and alternative energy sources is based not only on an economic justification, but also on the dynamics of the use of alternative energy sources. The authors show that in this case, the risk component is also laid in the tariff structure to overcome possible downturns in energy generation. The practical significance of the study is determined by the fact that the economic importance of the tariff for the integrated use of energy sources of various origins will increase the efficiency of the general regulation of the industry.

摘要： This study aims to determine the hydroxymethylfurfural (HMF) content in the honey samples which are processed at different temperatures by using a spectrophotometric method and to investigate the suitability of image processing method as an alternative method to spectrophotometry. Honey samples were subjected to conventional heating by using a conventional oven at 75 degrees C, 100 degrees C, 125 degrees C and 150 degrees C for 20 min. The HMF content of the samples were determined by using spectrophotometric method and the images of heated honey samples in tubes were captured by a professional camera simultaneously to compare results obtained from spectrophotometry and image processing. The results showed a correlation (y = 18.54x-1.224, R-2 = 0.987) between the data obtained by image processing and spectrophotometric analysis. Thus, it was concluded that, image processing can be used as an alternative method for determining the amount of HMF content in honey for different temperatures. Practical applications With the development of image processing technologies, there is a great potential for food safety and food preservation. This study will bring about improvement in the field of food quality and preservation analysis for food manufacturers. Moreover, this application can be used in food industry for a quick quality determination for mass production.