关键词： Adaptation   Ecological Genetics   Fish   Transcriptomics  

摘要： Identifying the molecular mechanisms contributing to phenotypic variation in natural populations is a major goal of molecular ecology. However, the multiple regulatory steps between genotype and phenotype mean that many potential mechanisms can lead to trait divergence. To date, the role of transcriptional regulation in local adaptation has received much focus, as we can readily measure mRNA quantity and have a reasonable grasp of how variation in the expression of many protein-coding genes can influence phenotype. Thus, studying the evolution of protein-coding gene mRNA abundance in candidate tissues has led to successes in detecting the molecular mechanisms underlying local adaptation (reviewed by Hill et al., 2021). However, the contribution of differential splicing of precursor mRNA (pre-mRNA) to adaptive differentiation, as well as the loci controlling this variation, remains largely unexplored in wild populations. In their "From the Cover'" article in this issue of Molecular Ecology, Jacobs and Elmer (2021) reanalyse muscle RNA sequencing (RNA-seq) data to quantify the relative contributions of variation in mRNA quantity (differentially expressed "DE" genes) and splice variant identity (differentially spliced "DS" genes) to parallel divergence of wild "benthic" and "pelagic" ecotypes of a salmonid fish, the Arctic charr (Salvelinus alpinus). They found little overlap in the identity and biological functions of DE and DS genes, suggesting that these two regulatory mechanisms act on different cellular traits to complementarily alter organismal phenotype. Furthermore, many DE and DS genes could be mapped to cis-acting QTL, arguing that some of this regulatory divergence is genetically based. DE and DS genes were also more likely to be "hub genes" than their nondivergent counterparts, hinting that this regulatory variation may have a variety of phenotypic effects. The comparison of three independently evolved pairs of benthic and pelagic charr uncovered greater th

关键词： alginates   alternative   dextran   gelatin   honey   hydrogels   natural   phytochemicals   polymers   polyurethane   wound care  

摘要： Effective wound healing is achieved by well-timed host, cell, and environment interactions involving hemostasis, inflammation, formation of repaired dermal structures, and epithelialization, followed by months to years of scar remodeling. Globally, various natural or synthetic agents or dressings are used to optimize wound environments, prolong drug release, aid in fluid absorption, provide favorable healing environments, and act as a mechanical barrier against wound trauma. In this scoping review of evidence from the PubMed and *** databases, authors examined clinical study evidence supporting the efficacy and safety of selected phytochemicals, vehicles, polymers, and animal products considered "naturally derived" or "alternative" wound interventions to provide a summary of preclinical evidence. Agents with the most clinical evidence were honey, alginates, polyurethane, gelatin, and dextran. Practice implications are described in the context of the TIMERS clinical paradigm.

摘要： Background and aims IAD is an uncommon and underdiagnosed disease, the aim is to identify its pathophysiological mechanisms and possible alternative therapies. Methods Eight patients (pts.), referred to the Emergency Department with focal signs, w...

关键词： Butter catfish (O   pabda)   Earthworm meal   Growth performance   Feed utilization   Hematology   Proximate chemical composition  

摘要： An experiment was carried out to determine the effect of replacing dietary fish meal protein with earthworm meal protein on growth performance, whole-body composition, and hematology of butter catfish (Ompok pabda). This experiment was conducted in 15 glass aquaria (200 L) with five treatments, each having three replications. Each of the aquaria was stocked with 40 fishes (average weight 0.62 g). The fish were fed with iso-nitrogenous diets having 35% protein (21% protein from fish meal and/or earthworm meal) twice a day up to satiation with the experimental diets, where fish meal protein was replaced by earthworm meal protein at 0% (control), 25%, 50%, 75%, and 100% in the treatments E-0, E-25, E-50, E-75, and E-100, respectively. At the end of the 8-week rearing period, the best growth performances including weight gain (5.23 +/- 0.14 g), percent weight gain (844.15 +/- 6.38%), and specific growth rate (4.00 +/- 0.11% day(-1)), and best feed utilization parameters, viz., feed conversion ratio (1.57 +/- 0.03) and protein efficiency ratio (2.87 +/- 0.04), were found in treatment E-75, where 75% of fish meal protein in the diet was replaced with earthworm meal protein. The hematological parameters showed no abnormality due to supplementation of earthworm meal in the diets. At the end of the experiment, whole-body carcass composition, viz., protein, lipid, and ash contents (15.93 +/- 0.19%, 7.22 +/- 0.60% and 2.14 +/- 0.10%, respectively), of O. pabda was higher in treatment E-75 compared to control (14.8 2 +/- 0.34, 6.16 +/- 0.27, 1.89 +/- 0.10, respectively). The results imply that earthworm meal can be used as a protein-rich source to replace fish meal, the major protein source, in the diet of O. pabda up to 75% level without compromising growth, immunity, and body composition.

关键词： liquid biopsy   circulating tumor DNA   high-throughput nucleotide sequencing   urinary bladder neoplasms   urine  

摘要： Purpose: Next-generation sequencing (NGS)-based profiling of both urinary tumor DNA (utDNA) and circulating tumor DNA (ctDNA) shows promise for noninvasive detection and surveillance of urothelial bladder cancer (UBC). However, the analytical performance of these assays remains undefined in the real-world setting. Here, we sought to evaluate the concordance between tumor DNA (tDNA) profiling and utDNA or ctDNA assays using a UBC patient cohort from the intended-use population. Materials and Methods: Fifty-nine cases with pathologically confirmed disease and matching tissue/urine pairs were prospectively enrolled. Baseline peripheral blood mononuclear cell and plasma specimens were collected during clinic visits. The Predicine CARE (TM) NGS assay was applied for ultra-deep targeted sequencing and somatic alteration identification in tDNA, utDNA and ctDNA. Results: Diverse quantitative metrics including cancer cell fraction, variant allele frequency and tumor mutation burden were invariably concordant between tDNA and utDNA, but not ctDNA. The mutational landscapes captured by tDNA or utDNA were highly similar, whereas a considerable proportion of ctDNA aberrations stemmed from clonal hematopoiesis. Using tDNA-informed somatic events as reference, utDNA assays achieved a specificity of 99.3%, a sensitivity of 86.7%, a positive predictive value of 67.2%, a negative predictive value of 99.8% and a diagnostic accuracy of 99.1%. Higher preoperative utDNA or tDNA abundance correlated with worse relapse-free survival. Actionable variants including FGFR3 alteration and ERBB2 amplification were identified in utDNA. Conclusions: Urine-based molecular pathology provides a valid and complete genetic profile of bladder cancer, and represents a faithful surrogate for genotyping and monitoring newly diagnosed UBC.

关键词： Cardiac arrest   Cardiopulmonary resuscitation   Pulse rate   Pulse oximetry plethysmographic waveform   Feedback device  

摘要： Feedback indicators can improve chest compression quality during cardiopulmonary resuscitation (CPR). However, the application of feedback indicators in the clinic practice is rare. Pulse oximetry has been widely used and reported to correlate spontaneous circulation restoration during CPR. However, it is unclear if pulse oximetry can monitor the quality of chest compression. We hypothesized that pulse rate monitored by pulse oximetry can be used as a feedback indicator of the chest compression rate during CPR in a porcine model of cardiac arrest. Seven domestic male pigs (30-35 kg) were utilized in this study. Eighteen intermittent chest compression periods of 2 min were performed on each animal. Chest compression and pulse oximetry plethysmographic waveforms were recorded simultaneously. Chest compression and pulse rates were calculated based on both waveforms. Compression interruption and synchronous pulse interruption times were also measured. Agreement was analyzed between pulse rates and synchronous chest compression rates, as well as between compression interruption times and synchronous pulse interruption times. A total of 126 compression periods of 2 min were performed on seven animals. Interclass correlation coefficients and Bland-Altman analysis revealed reliable agreement between pulse rates and synchronous chest compression rates. Similarly, compression interruption and synchronous pulse interruption times obtained also showed high agreement. Pulse rate can be used as an alternative indicator of chest compression rate during CPR in a porcine model of cardiac arrest. Pulse interruption time also can be used to reflect compression interruption time precisely in this model.

关键词： varicocele   ultrasound   semen analysis  

摘要： Purpose: We assessed the role of standing vs supine scrotal ultrasound (SUS) for varicocele assessment by evaluating differences in clinical outcomes. Materials and Methods: We retrospectively reviewed men from 2008-2020 diagnosed with varicocele who had documented SUS with both supine and standing assessments with and without Valsalva. Clinical outcomes (semen parameters, TUNEL and serum testosterone [T]) after microsurgical varicocelectomy were compared among men who had varicoceles diagnosed by standing SUS (vein size >2.5 mm, vein size >3.0 mm or reversal of flow) to those who would have been missed on supine SUS only. Results: A total of 349 men underwent varicocelectomy (right: 5 [1.4%];left: 118 [33.8%];bilateral: 226 [64.8%]). Disagreement between those with abnormal standing vs normal supine for vein size >2.5 mm was: 56 men (16.1%) on the right and 31 men (8.9%) on the left, for vein size >3.0 mm was: 64 men (18.3%) on the right, and 56 men (16.1%) on the left, and for flow reversal was: 36 (14.0%) on the right and 40 (15.4%) on the left. For those >2.5 mm, only T had significant improvements on the left (p=0.05). For those >3.0 mm significant differences were seen for sperm motility on the right (p=0.04), and TUNEL (p=0.04) and T (p <0.01) on the left. For flow reversal, significant differences were seen for serm concentration (p<0.01), morphology (p[0.03) and volume (p=0.05) on the right and TUNEL on the left (p=0.02). Conclusions: Standing SUS identifies a greater number of men who would have been missed using supine SUS only.

摘要： The nucleobase derivative 5-aminouracil (AUr, C4H5N3O2) is of interest for its biological activity, yet the solid state structure of this compound has remained elusive owing to its propensity to crystallize as aggregates of microcrystalline particles. Here we report the first single-crystal structure of AUr determined from synchrotron x-ray diffraction data. An early crystal structure prediction effort, which assumed that AUr was rigid in the isolated molecule optimized conformation, provided several poor matches to the simulated PXRD pattern. Revisiting these crystal structures, by periodic electronic level modelling (PBE-TS optimization) gave more realistic relative lattice energies, but a good match to the experimental powder pattern required using the experimental cell parameters. PXRD and Raman spectroscopy suggest that phase impurities may be present in the bulk crystallization product, though the identity of alternative polymorphs could not be confirmed on the basis of the data available.

关键词： 3' mRNA-Seq   alternative polyadenylation   Large White pigs   porcine reproductive and respiratory syndrome virus   Tongcheng pigs  

摘要： Alternative polyadenylation (APA) is a widespread post-transcriptional regulation mechanism that increases the biological complexity of transcriptome and proteome. However, it is unclear whether APA regulation plays a role in genetic resistance to porcine reproductive and respiratory syndrome virus (PRRSV). Here, we reported genome-wide APA regulation of porcine alveolar macrophages in PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs upon PRRSV infection. Using 3' mRNA sequencing strategy, we detected 75 981 high-quality APA sites in porcine alveolar macrophages of TC and LW pigs. Furthermore, 1202 and 1089 differentially expressed APA sites, as well as 79 and 117 untranslated region-APA switching genes were identified in TC pigs and LW pigs upon PRRSV infection respectively. The APA events in TC pigs and LW pigs were involved in different biological pathways, while APA events in TC pigs are directly associated with the immune response to PRRSV infection. In addition, we identified genetic variations affecting polyadenylation signal between TC pigs and LW pigs. These findings would provide helpful information on APA regulation for further understanding of genetic resistance to PRRSV.

关键词： Household fermentable waste   cement industry   alternative fuel   life cycle assessment   life cycle costing  

摘要： The scope of this paper is to examine the environmental and economic performance of an alternative household fermentable waste (HFW) management paradigm, developed within the framework of the HORIZON 2020 project Waste4think. In Greece, the business-as-usual scheme for the management of HFW is its disposal in landfills as part of mixed waste. Waste4think developed an alternative approach based on the benefits of source separation. Specifically, source separated HFW is taken to a drying/shredding plant, located in the municipality, for the production of a high-quality biomass product called FORBI. Alternative approaches have been examined for the exploitation of FORBI. In this work, the use of FORBI as an alternative fuel for the cement industry is assessed using life cycle assessment (LCA) and life cycle costing (LCC) tools. The results show that the proposed HFW management framework performs better than the baseline scenario both in economic and environmental terms. However, focus should be given to the optimisation of the drying/shredding process in order to reduce its energy intensity and environmental loadings.