关键词： 金堂方言   构词重叠   语义功能   形成动因  

摘要： 重叠是西南官话金堂方言重要的构词手段。文章对金堂方言重叠式名词进行全面考察,发现其重叠形式主要有"AA""ABB""AAB""AABB"等四种类型,其中"AA"占重叠式名词总数的一半以上,部分"AA"式用普通话"A""A子"表示后词义有差异,并且有一半的重叠式名词可用子缀词表示且词义基本不变。重叠式名词不仅有成词、变类、转义三种基本语义功能,还有变量和描绘性附加功能,其形成的动因主要与文化背景、语言类推和认知机制中的隐喻、转喻以及象似性原则有关。

关键词： ABO血型   B(A)亚型   血型血清学   基因分型  

摘要： 目的对3例血清学定性为A_(weak) B亚型的血标本进行ABO基因检测,探讨其分子遗传学特点,并探讨B(A)亚型与CisAB、A亚B型的鉴别要点。方法用PCRSSP对常规血清学方法定型困难的3例标本进行ABO基因分型,并对其ABO基因第6、7外显子扩增后进行直接测序及单倍型测序,确定其基因型。结果3例标本血型血清学结果均为A_(weak) B型,存在不规则抗A抗体。基因分型均为B(A)04型,其B等位基因在第7外显子存在nt640A>G突变,标本1为B(A)04/O02,标本2、3为B(A)04/O01。结论当血清学检测结果正反定型不一致时,应采用分子诊断技术进行辅助诊断,并采用配血相合输血策略,可确保临床输血安全。

摘要： The goal of this research was to compare clinical signs, symptoms and laboratory findings in children diagnosed with Kawasaki disease and those with multisystem inflammatory syndrome in children (MIS-C) associated with coronavirus disease 2019 (COVID-19).We reviewed medical records of children diagnosed with Kawasaki disease or MIS-C hospitalised in the University Hospital for Infectious Diseases ‘’Dr. Fran Mihaljević’’ in the period from February 25th 2020 to February 24th 2021. We defined MIS-C using World Health Organisation *** were 13 children diagnosed with Kawasaki disease and 23 children diagnosed with MIS-C hospitalised in this period. The average duration of hospitalisation was similar in both groups, approximately 8 days. Boys were overall more affected (66%) than girls. Children with MIS-C were older and more often gastrointestinal symptoms were present. When comparing laboratory results, children in the MIS-C group had higher C-reactive protein levels and lower platelet count. Also, they required intensive care treatment more frequently. The first therapy of choice for all children with Kawasaki disease was monotherapy with intravenous immunoglobulins (IVIG). Monotherapy with IVIG was used in 15 patients with MIS-C, while 8 of them were initially treated with the combination of IVIG and corticosteroids. One death case occured in the MIS-C group, and none in the Kawasaki *** inflammatory syndrome in children associated with COVID-19 is a new pediatric disease that is dangerous and potentially lethal. Although many clinical and laboratory findings overlap with Kawasaki disease, this disease requires intensive care treatment more often. It can also have a fatal outcome – therefore prompt recognition and early treatment are crucial.

关键词： 扦插育苗   育种技术   育苗容器  

摘要： 我国遵循可持续发展的理念,近年来不断开展植树造林活动,在造林工程中,育苗和育种是影响苗木成活率和整体质量的关键环节。育苗方法及育种技术是未来造林行业需要研究的一个重要课题,对我国未来林业生态环境保护工程产生着巨大的影响。为了促进苗木的健康生长,文章针对林业扦插育苗方法、容器育苗、分植育苗等多种育苗技术进行研究和讨论,以供参考。

关键词： antiretroviral therapy   HIV   IL-17A   IL-32   intestinal epithelial cells  

摘要： Objectives: Untreated HIV infection was previously associated with IL-32 overexpression in gut/intestinal epithelial cells (IEC). Here, we explored IL-32 isoform expression in the colon of people with HIV (PWH) receiving antiretroviral therapy (ART) and IL-32 triggers/modulators in IEC. Design: Sigmoid colon biopsies (SCB) and blood were collected from ART-treated PWH (HIV + ART;n = 17;mean age: 56 years;CD4(+) cell counts: 679 cells/mu l;time on ART: 72 months) and age-matched HIV-uninfected controls (HIVneg;n = 5). The IEC line HT-29 was used for mechanistic studies. Methods: Cells from SCB and blood were isolated by enzymatic digestion and/or gradient centrifugation. HT-29 cells were exposed to TLR1-9 agonists, TNF-alpha, IL-17A and HIV. IL-32 alpha/beta/gamma/D/epsilon/theta and IL-17A mRNA levels were quantified by real-time RT-PCR. IL-32 protein levels were quantified by ELISA. Results: IL-32 beta/gamma/epsilon isoform transcripts were detectable in the blood and SCB, with IL-32 beta mRNA levels being predominantly expressed in both compartments and at significantly higher levels in HIV + ART compared to HIVneg. IL-17A transcripts were only detectable in SCB, with increased IL-17A levels in HIVneg compared with HIV + ART and negatively correlated with IL-32 beta mRNA levels. IL-32 beta/gamma/epsilon isoform mRNA were detected in HT-29 cells upon exposure to TNF-alpha, Poly I:C (TLR3 agonist), Flagellin (TLR-5 agonist) and HIV. IL-17A significantly decreased both IL-32 beta/gamma/epsilon mRNA and cell-associated IL-32 protein levels induced upon TNF-alpha and Poly I:C triggering. Conclusion: We document IL-32 isoforms abundant in the colon of ART-treated PWH and reveal the capacity of the Th17 hallmark cytokine IL-17A to attenuate IL-32 overexpression in a model of inflamed IEC.

关键词： 衢枳壳   功能性消化不良   胃排空   小肠推进   胃泌素   胃动素   生长抑素   大鼠  

摘要： 目的:比较衢枳壳与其他三大主产区枳壳对功能性消化不良(FD)模型大鼠理气作用的差异。方法:56只雄性SD大鼠随机分为正常组、模型组、阳性药组、衢枳壳组、江枳壳组、川枳壳组、湘枳壳组,每组8只。“不规则喂养法结合夹尾刺激法”复制FD模型,连续给药14 d,观察记录各组大鼠一般状态、24 h代谢值变化;末次给药后禁食12 h,用半固体营养糊法检测各组大鼠小肠推进率和胃残留率;酶联免疫法(ELISA)检测血浆胃泌素(GAS)、胃动素(MTL)、生长抑素(SS)含量。HE染色观察胃窦组织病理学改变。结果:与正常组比较,模型组大鼠的体质量、24 h饮水量与摄食量、胃排空率及小肠推进率、血浆GAS、MTL含量明显降低,SS含量明显升高。与模型组比较,经4组枳壳干预后,各给组大鼠的体质量、24 h饮水量与摄食量、胃排空率及小肠推进率、血浆GAS、MTL含量明显升高,SS含量明显降低。与衢枳壳组相比,江枳壳组、川枳壳组与湘枳壳组大鼠胃排空率与小肠推进率显著降低(P<0.05或P<0.01),其他指标比较均无显著性差异(P>0.05)。结论:四个主产区枳壳对FD模型大鼠均有理气作用,衢枳壳对FD模型大鼠的理气作用优于江枳壳、川枳壳和湘枳壳,江枳壳、川枳壳和湘枳壳对FD模型大鼠的理气作用无显著性差异。

关键词： 聂耳   角色   理解   创造   熟悉  

摘要： 壹理解角色固不易.理解自己则愈难.我不知道十年前要我来扮演聂耳会是什么样子我熟悉聂耳,如同熟悉我最亲近的人.但是熟悉的.却不一定是最理解的.对演员来说.熟悉和理解是创造的起点.二者缺一,都会使未来的角色成为跛行人物.