关键词： Dipyridamole   DNA binding   Genotoxicity   Cytotoxicity   Anti-cancer  

摘要： The molecular mechanism behind the anti-cancer potential of dipyridamole, an antiplatelet drug was investigated by examining the interaction of dipyridamole with DNA as well as the genotoxicity and cytotoxicity associated with dipyridamole . Interaction of dipyridamole with calf thymus DNA (Ct-DNA) was determined in order to ascertain the strength and mode of binding as well as the thermodynamic parameters involved. Dipyridamole interacts with Ct-DNA through a groove binding mechanism. Furthermore, a preliminary assessment of the genotoxic effect of dipyridamole was performed in vitro using the plasmid nicking assay and comet assay. Genotoxicity was also confirmed through DAPI nuclear staining of HeLa and HCT-116 cells. Cytotoxicity was assessed in vitro on HeLa, HCT-116 as well as HEK293 cells using the MTT assay as well as through inverted microscopy. Dipyridamole was selectively more toxic towards cancer cells. Dipyridamole demonstrated induction of micronucleus and chromosomal aberrations only at high concentrations in bone marrow cells of male Wistar rats, suggesting its safe usage at clinically relevant concentrations. These results indicated the existence of an intrinsic genotoxic potential of dipyridamole which may be contributing to its anti-cancer properties . (c) 2021 Elsevier B.V. All rights reserved.

关键词： Alternative preservatives   Cosmetic products   Gas chromatography-mass spectrometry   Hydroxylated compounds   Ultrasound-assisted leaching  

摘要： Some hydroxylated compounds commonly used in cosmetic formulations including short chain glycols, benzylic alcohols, and organic acids show antimicrobial activity, although they are not considered as preservatives according to the existing European legislation. These 'alternative preservatives' are not exempt of potential side-effects for cosmetics users. The aim of this work is to develop a simple and affordable analytical method useful for the simultaneous and green determination of fourteen compounds used as 'alternative preservatives' in cosmetic samples with different matrices. The proposed method allows a rapid sample preparation by simple dissolution or dispersion of the sample in ethanol using ultrasound-assisted leaching of the analytes from the cosmetic matrix. Gas chromatography coupled to mass spectrometry (GC-MS) is used for the analysis of the samples. Working conditions for the instrumental measure and the quantification of analytes were studied. The method was found to have high sensitivity and good precision (relative standard deviation below 13%) as well as low limits of detection (i.e., 0.01.10(-3)-2.14.10(-3)% w/w) and quantification (i.e., 0.04.10(-3)-7.14.10(-3)% w/w). The method was successfully applied to five commercial cosmetic samples of different composition. Recovery values near 100% were obtained. Each sample was found to contain at least three of the analytes of the study and their concentrations were determined with low standard deviations. The analytical features of the proposed method and the obtained results agree with the principles of Green Analytical Chemistry and make it a useful tool for controlling these alternative preservatives in the cosmetic industry in order to guarantee quality and safety of the products. (C) 2021 The Authors. Published by Elsevier B.V.

关键词： adenosine   cardiac arrest   palpitations   electrocardiogram   half-life   adult   emergency service   hospital   intravenous injections   randomization   arm   syringes   paroxysmal supraventricular tachycardia   lightheadedness   risk   attributable   absolute risk reduction   transverse plane   medical records review   research funding   intention to treat analysis   primary outcome measure  

摘要： Background An intravenous adenosine with a double syringe technique is an essential treatment in a patient with paroxysmal supraventricular tachycardia (PSVT). Due to its fast metabolisation (approximate half-life is about 2-5 seconds), it required a fast intravenous injection to make the best outcome in treatment. In some practice, there is an alternative method to administer adenosine by elevating the arm perpendicular to a horizontal plane after injection to facilitate a rapidly injected manner. However, there is no scientific proof of this hypothesis. Purpose To compare the successful termination of PSVT with the first dose of intravenous adenosine between usual and the alternative methods. We hypothesised that the alternative method is superior to the usual method. Methods We conducted a pilot randomised controlled trial in the emergency department. Eligible patients were adult patients with stable PSVT who failed termination by vagal manoeuvre. Eligible patients were randomised with variable block sizes (block sizes 2, 4) and allocate into the usual group (double syringe technique of 6 milligrams adenosine), or the alternative group (same as the usual method, then immediately followed by elevating arm to 90 degrees perpendicular to a horizontal plane for 10 seconds). We estimated that an alternative method would increase the success rate by about 5%. We would need to enrol 700 patients. However, a pilot chart review in our setting showed about 50 patients per year were eligible. At this rate of enrolment, the number of cases would not be feasible to achieve in the available period. Therefore, we conduct this study as a pilot study. The primary outcome is the success rate in terms of termination of PSVT on an electrocardiogram (ECG). Secondary outcomes are complications within a minute after adenosine administration. We used intention-to-treat analysis. Results Thirty patients were randomised and allocated in each group. The success rate was 86.7 % in the alter

关键词： alternative splicing   pluripotency   RNA-binding protein   ZFP207   Zinc finger protein  

摘要： The pluripotent state is not solely governed by the action of the core transcription factors OCT4, SOX2, and NANOG, but also by a series of co-transcriptional and post-transcriptional events, including alternative splicing (AS) and the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs. Zinc Finger Protein 207 (ZFP207) is an essential transcription factor for mammalian embryonic development. Here, we employ multiple functional analyses to characterize its role in mouse embryonic stem cells (ESCs). We find that ZFP207 plays a pivotal role in ESC maintenance, and silencing of Zfp207 leads to severe neuroectodermal differentiation defects. In striking contrast to human ESCs, mouse ZFP207 does not transcriptionally regulate neuronal and stem cell-related genes but exerts its effects by controlling AS networks and by acting as an RBP. Our study expands the role of ZFP207 in maintaining ESC identity, and underscores the functional versatility of ZFP207 in regulating neural fate commitment.

关键词： 益生菌   替抗产品   肉鸡   联合应用技术  

摘要： 抗生素使用在肉鸡养殖业中起到了不可替代的作用,而其耐药性的产生、对环境的污染已引起全行业人的重视,新抗生素的研发速度远远落后于耐药菌进化速率,不得不让人类发展与应用能够规避其缺陷的产品。肉鸡业是全球增长速度较快行业之一,伴随我国饲料禁抗令的频布与实施,许多的替抗产品应运而生,但效果不一。为了保障经济效益和高质量需求,不同替抗产品之间的联合应用技术开始不断地探索与尝试。本文综述了国内外益生菌与其他替抗产品在肉鸡上联合应用技术,以期为生产提供理论借鉴。

关键词： 蝴蝶兰   花朵数量   相关分析   通径分析  

摘要： 为研究蝴蝶兰花朵数的遗传变异特性,探索与蝴蝶兰花朵数形成相关的重要数量性状,合理有效利用种质资源,本研究以25个大花型红色蝴蝶兰品种为试材,依次测量19个重要性状进行相关分析,并建立回归方程,将其对花朵数的影响效果进行通径分析。结果表明:花朵数和花序长极显著正相关,而花序长、花朵间距都与株幅显著正相关。花序长对花朵数的直接通径系数最大,为1.318,达到显著水平;对花朵数的多元回归方程为:Y=6.709+0.314X_(8)-0.793X_(12)-0.125X_(7)+0.075X_(6)(Y为花朵数,X_(8)为花序长,X_(12)为花朵间距,X_(7)为花梗高,X_(6)为花梗长度)。综合可知,花序长是直接作用花朵数的主要因素,而花朵间距是间接作用花朵数的主要因素。

关键词： 头孢类抗菌药物   联合药物   不良反应   评价分析  

摘要： 讨论头孢类抗菌药物联合其他药物导致不良反应的具体情况。方法:选择我院2020年7月到2021年7月之间收治的因为使用头孢类抗菌药物联合其他药物导致不良反应的78例患者,利用回顾性分析的方式,针对其合并用药的种类、合并用药之前和之后的实际不良反应的发生情况等进行分析。结果:在全部78例患者中,使用头孢他啶、水电解质及酸碱平衡药物分别是联合用药时候不良反应发生率最高的两种药物,分别占比为61.5%和50%。患者以全身性的反应的发生率为最高,占比为71.8%,显著高于其他组织系统,并有联合用药之后不良反应的发生率显著高于联合用药之前的情况,差异具有统计学意义,P>0.05。其中,因为治疗方案不合理会导致不良反应的共有26例,占比为33.3%,又以用法不放当的占比为最高,占比为42.3%。结论:使用头孢类的抗菌药物联合其他的药物会在某种程度上增加相关不良反应的发生率,相关医务人员要提升重视的程度,利用分析联用药物不良反应的发生情况,可以为临床的合理用药提供有效的依据。

关键词： 老虎   清朝   羡慕   危险  

摘要： 老虎凶猛无比,被人们称为动物之王,人类对其既害怕又羡慕。人类况之于己,成年健壮谓之虎背熊腰,小儿可爱称为虎头虎脑,这是羡慕;危险逼近是虎视眈眈,经历危险为虎口余生。人们还将自己的身体与虎相比,如手有虎口、口中生了虎牙等。老虎进入了人类的生活,也进入了清朝审理命案的刑科题本.

关键词： 其他感染性腹泻   流行特征   福州市  

摘要： 目的了解福州市其他感染性腹泻报告病例的流行特征,为预防控制提供科学依据。方法通过中国疾病预防控制信息系统“传染病报告信息系统”、“突发公共卫生事件管理信息系统”收集2015-2020年福州市报告的其他感染性腹泻病例相关资料,采用描述性流行病学方法进行分析。结果2015-2020年福州市报告其他感染性腹泻病病例22388例,死亡病例4例;报告其他感染性腹泻暴发疫情共14起;各年报告发病率之间差异有统计学意义(P<0.01),年均报告发病率51.51%。发病高峰出现在每年的冬春季(12月至次年3月);全市以仓山区、马尾区及闽清县发病率较高,发病率在74.07/10万~120.31/10万。男性、女性发病率分别为60.29/10万、42.51/10万,男女发病率之间差异具有统计学意义(P<0.01);病例主要集中在10岁以下儿童,占总病例数的86.22%。病原学检测514份样本中,检测出诺如病毒感染240例(46.69%),轮状病毒感染204例(39.69%)。结论福州市其他感染性腹泻发病具有明显的季节性,且重点人群明确,为10岁以下儿童多见,诺如病毒是常见的致病因子。