摘要： Macrophages perform key and distinct functions in maintaining tissue homeostasis by finely tuning their activation state. Within the tumor microenvironment, macrophages are reshaped to drive tumor progression. Here we report that tumor necrosis factor a-induced protein 8-like 1 (TIPE1) is highly expressed in macrophages and that depletion of TIPE1 impedes alternative activation of macrophages. TIPE1 enhanced activation of the PI3K/Akt pathway in macrophages by directly binding with and regulating the metabolism of phosphatidylinositol4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5trisphosphate (PIP3). Accordingly, inhibition of the PI3K/Akt pathway significantly attenuated the effect of TIPE1 on macrophage alternative activation. Tumor-associated macrophages (TAM) in human liver cancer and melanoma tissues showed significantly upregulated TIPE1 expression that negatively correlated with patient survival. In vitro and in vivo, TIPE1 knockdown in macrophages retarded the growth and metastasis of liver cancer and melanoma. Furthermore, blockade or depletion of TGFI3 signaling in macrophages abrogated the effects of TIPE1 on tumor cell growth and migration. Together, these results highlight that the phosphoinositide-related signaling pathway is involved in reprogramming TAMs to optimize the microenvironment for cancer progression.

关键词： Nuclear magnetic resonance spectroscopy  

摘要： The conflict between alternative crystal structures in the Cambridge Structural Database for the diuretic drug indapamide hemihydrate (IND) has been resolved with the aid of C-13 solid-state NMR. IND is seen to contain multiple distinct molecules in the asymmetric unit (Z ' = 4) rather than exhibiting disorder in the orientation of sulfonamide groups. The NMR crystallographic approach is a more effective tool for distinguishing between alternative structures than naive judgements of quality based on crystallographic refinement agreement factors.

关键词： Alternative protein   bioactivity   buckwheat   digestibility   functional properties   protein  

摘要： The need for protein in human nutrition is rapidly increasing because of the increasing world population and consumer preference for high-protein foods. Plant proteins are gaining attention as sustainable means of meeting the global protein need due to their lower carbon footprint. Nonetheless, the food industry has neglected or underutilized many plant proteins, including buckwheat protein. Buckwheat is a pseudocereal and its groats contain beneficial components such as proteins, dietary fiber, vitamins, and bioactive polyphenols. The protein quality of buckwheat seeds varies between the tartary and common buckwheat types;both are gluten-free and contain considerable amount of indispensable amino acids. This review provides a detailed discussion on the profile, amino acid composition, digestibility, allergenicity, functional properties, and bioactivity of buckwheat proteins. Prospects of processing buckwheat for improving protein digestibility and deactivating allergenic epitopes were also discussed. Based on the literature, buckwheat protein has a tremendous potential for utilization in structuring food products and developing peptide-based functional foods for disease prevention. Future research should develop new processing technologies for further improvement of the quality and functional properties of buckwheat protein in order to facilitate its utilization as an alternative plant-based protein toward meeting the global protein supply.

关键词： acupuncture   functional constipation   laxative   massage   meta-analysis  

摘要： Background: The efficacy of alternative therapies and medications for functional constipation (FC) in adults is well established, however, the efficacy and safety of different alternative therapies and medications for FC in adults is not fully clarified. Due to there are many different alternative therapies and medications available for the treatment of febrile FC in adults, the selection of appropriate alternative therapies and medications has become an urgent issue. The purpose of this study was to evaluate the effectiveness and safety of alternative therapy and medicine for FC in adults. Methods: PubMed, Web of Science, Scopus, Cochrane Library, Embase, China National Knowledge Infrastructure, China Science and Technology Journal Database and Wanfang Data were searched to identify randomized controlled trials which focused on alternative therapy and medicine for FC in adults from December 15, 2020 to July 1, 2021. Subsequently, 2 researchers will be independently responsible for literature screening, data extraction, and assessment of their quality. This study uses The R Programming Language 4.0.2 based on Bayesian framework for NMA. Odds ratios or standardized mean differences will be modeled using Markov chain Monte Carlo methods, both with 95% confidence intervals. Results: The results of this meta-analysis will be submitted to a peer-reviewed journal for publication. Conclusions: The conclusion of this systematic review will provide evidence for selecting an optimal alternative therapy and medicine for patients with FC in adults. Ethics and dissemination: The protocol of the systematic review does not require ethical approval because it does not involve humans. This article will be published in peer-reviewed journals and presented at relevant conferences. Systematic review registration: INPLASY202210091.

关键词： 储气工程   其他费用   费用定额   影响  

摘要： 国家石油天然气管网集团有限公司为助力实现“碳达峰、碳中和”目标,在天津、粤东、深圳、龙口、漳州等接收站项目中,加快推进基础设施的建设,多个储气工程可行性研究项目同步开展。储气工程费用主要为储罐投资,但储罐投资基本上已很明确,对储气工程投资差异不大;而其他费用因套用不同的费用定额,投资差异较大。文章主要从其他费用方面说明不同的费用定额对储气工程投资的影响,及分析影响其他费用数值的主要因素。

关键词： Silicon dioxide   Silica-binding peptides   His-tag   Affinity purification   immobilization   Batch affinity chromatography  

摘要： "Green" protein purification/immobilization processes based on low-cost, earth-abundant, and eco-friendly affinity matrices are highly desirable. Unmodified silica matrices fit well these demands. Since histidine-rich silica binding peptides are frequently isolated in biopanning experiments, this work aimed at assessing the viability of using bare silica as an alternative matrix for the purification/immobilization of His-tagged proteins. Adsorption and desorption studies with a purified His6-tagged EGFP shown that binding to bare silica particles of different size and porosity occurred under the conditions tested, and that elution could be accomplished with eco-friendly eluants containing L-arginine/L-lysine. Non-tagged EGFP did not bind to these matrices. Small-scale batch purification schemes using silica gel Davisil grade 643 or 646 as affinity matrices and a Tris-buffered saline eluant containing 0.5 M L-arginine (pH 8.5) allowed purifying His6-EGFP from Escherichia coli lysates with a purity of up to 96% and a recovery yield of ~70% after just one elution step. EGFP tagged with the silica-binding peptide Car9 was recovered with comparable purity and yield. Other His-tagged proteins could also be purified to similar purity levels. The scale of this batch purification scheme was shown to be extendable. These results demonstrate that unmodified silica matrices can be used to effectively purify His-tagged proteins. Since the recovery of double tagged His6-EGFP-Car9 was only of 30-55%, the combination of tags revealed to be advantageous for immobilization purposes.

关键词： 小学科学   学科融合   学生需求   教学评价  

摘要： 《义务教育小学科学课程标准(2017 版)》明确指出,综合性能力是小学生学习中很关键的能力,也是小学生在科学学习中要面对的一种基本学科特点。多学科融合已经成为学生学习中需要具备的一种能力,所以,在教学中,教师也要将这种融合能力渗透在学科中。多学科融合对于小学生的健康成长具有重要的意义。本文结合教学实际调查和经验分析,提出了当前小学科学与其他学科融合存在的问题,并提出了实际的教学对策。

关键词： 儿童   营养状态   维生素A   其他微量营养素  

摘要： 研究儿童自身营养状态受到维生素A复合其他微量营养素影响情况。方法:研究抽取120例入院儿童(纳入时间控制在2019年09月,结束时间控制在2021年09月时间段),以红篮球随机抽签方式实施分组,单纯维生素A补充60名儿童纳入对照组内,除对照组予以方式同时加入多种微量营养元素60名儿童纳入观察组,对比两组儿童最终提个检查以及各项血清指标差异。结果:干预前,对照组与观察组儿童出现轻微维生素A缺乏、消瘦、低体重、贫血、肥胖及生长迟缓率对比,无显著差异,(p>0.05);干预后,对照组儿童出现轻微维生素A缺乏、消瘦、低体重、贫血、肥胖及生长迟缓率显著高于观察组儿童,差异有统计学意义,(p<0.05)。干预前,对照组与观察组儿童血红蛋白、钙、维生素A以及锌指标水平对比,无显著差异,(p>0.05);干预后,对照组儿童血红蛋白、钙以及维生素A指标水平均显著低于观察组儿童,锌指标水平显著高于观察组儿童,差异有统计学意义,(p<0.05)。干预后,对照组儿童家属总满意率显著低于观察组,差异有统计学意义,(p<0.05)。结论:儿童自身营养状态受到维生素A复合其他微量营养素影响显著,及时有效补充可确保儿童营养状况得到有效改善,促使儿童后期能够健康成长。

关键词： 中药   白头翁散   最低抑菌浓度   最小杀菌浓度   大肠杆菌  

摘要： 研究中药白头翁散各组分(白头翁、黄柏、黄连、秦皮)以及其他6种中药对大肠杆菌的体外抑菌效果。用传统水煎法分别提取10种中药的有效成分;采用试管二倍稀释法测定单味中药、两药联合组成药对及自制白头翁散对大肠杆菌的最低抑菌浓度(MIC)以及最小杀菌浓度(MBC)。结果显示,白头翁散各组分均可有效抑制大肠杆菌,其中黄连的MIC为6.250 mg/mL,抑菌效果最佳。单味中药中对大肠杆菌杀菌效果较好的是黄连、秦皮、诃子,MBC均为200.000 mg/mL;药对中白头翁+黄连、大青叶+诃子对大肠杆菌的MIC均为3.125 mg/mL,抑菌效果最佳;白头翁+黄连对大肠杆菌的MBC为12.500 mg/mL,杀菌效果最佳。自制白头翁散对大肠杆菌的MIC为3.125 mg/mL、MBC为100.000 mg/mL。结果提示,白头翁散4种单药相加的抗菌效果优于单药和部分药对。

关键词： 国土测绘   遥感应用   卫星遥感数据   高分卫星   亚米级   高分遥感   高光谱   深度化  

摘要： 近年来,随着卫星遥感系统快速发展,系统性能不断提升,遥感应用向深度化、综合化方向发展,广泛应用于气象、农林、海洋水利、城市规划、国土测绘和应急灾害等多个领域,发挥了显著作用,并取得初步的应用成果。目前,在轨可用的国产高分遥感卫星有20余颗,涵盖多光谱、高光谱、亚米级、可见光、合成孔径雷达(SAR)等卫星遥感数据。